Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 52 issue 12 (december 2018) : 1745-1750

Hepatoprotective effect of seabuckthorn leaf extract in streptozotocin induced diabetes mellitus in Wistar rats

P. Khajuria, P. Raghuwanshi, A. Rastogi, A.L. Koul, R. Zargar, S. Kour
1Division of Veterinary Physiology and Biochemistry, Sher-e-Kashmir University of Agricultural Sciences and Technology R.S. Pura, Jammu-180 009, Jammu and Kashmir, India.
Cite article:- Khajuria P., Raghuwanshi P., Rastogi A., Koul A.L., Zargar R., Kour S. (2018). Hepatoprotective effect of seabuckthorn leaf extract in streptozotocin induced diabetes mellitus in Wistar rats. Indian Journal of Animal Research. 52(12): 1745-1750. doi: 10.18805/ijar.B-3439.
Study was conducted to evaluate the hepatoprotective effects of Seabuckthorn leaf extract (SLE) supplementation on serum enzymatic levels in streptozotocin (STZ) induced diabetes mellitus in Wistar rats. Thirty-two adult male Wistar rats were divided into four groups namely CON (negative control), SCO (Seabuckthorn control), DCO (Diabetic control), and DSL (Diabetic seabuckthorn treatment group). Diabetes mellitus was induced by single intra peritoneal injection of STZ @ 50 mg/kg body weight in DCO and DSL group of rats. SLE was administered orally @ 100mg/kg body weight for 40 days to SCO and DSL groups. CON served as the negative control. Blood samples were collected from experimental animals on zero, 20th, and 40th days of trial to study liver specific serum enzyme profile viz aspartate amino transaminase (AST), alanine amino transaminase (ALT), alkaline phosphatase (ALP) and acid phosphatase (ACP). Significantly (P<0.01) higher levels of all the enzymes studied were observed in experimentally induced diabetic rats in comparison to normal rats. However, in SLE treated diabetic rats (DSL group), significant (P<0.01) improvement was observed in all the above enzymes. It may be concluded that SLE exerts hepatoprotective effect in STZ induce Diabetes mellitus in Wistar rats.
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