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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 4 (april 2018) : 615-618,   Doi: 10.18805/ijar.B-3433

Effect of mineral supplementation on humoral immunity against rabies vaccine in dog pups

S
Sarita Devi
M
M.C. Sharma
R
R.P. Singh
U
U. Dimri
A
A.C. Patel
P
Pankaj Kumar
R
R.D. Singh
1Division of Medicine, Indian Veterinary Research Institute (IVRI), Izatnagar-243 122, Uttar Pradesh, India.
Cite article:- Devi Sarita, Sharma M.C., Singh R.P., Dimri U., Patel A.C., Kumar Pankaj, Singh R.D. (2017). Effect of mineral supplementation on humoral immunity against rabies vaccine in dog pups. Indian Journal of Animal Research. 52(4): 615-618. doi: 10.18805/ijar.B-3433.

ABSTRACT

The present study was aimed to determine the effect of a specially formulated mineral supplement in shaping the humoral immune response of dog pups in response to anti-rabies vaccination. Mineral supplement was formulated based on plasma analyzed for macro and micro minerals randomly collected from 226 dogs. Twelve mongrel/non-descript dog pups (3 – 6 months) randomly allocated into two groups (n=6) were tested for stimulation of humoral immune system by assessing the antibody titre against rabies vaccine using Rapid Fluorescent Focus Inhibition Test (RFFIT) and in vivo Mouse Neutralization Test (MNT) for antibody detection post supplementation of formulated mineral supplement upto 28 days. Early and higher antibody titre (>1:4096) was recorded on day seven itself in the group of dog pups fed with formulated mineral supplemented as compared to non-supplemented group. Present study indicated that mineral supplementation prior to the anti-rabies vaccination may elicit quick and high level of protective antibodies. The findings may also be important in the event of post bite immunization, in combination with this mineral supplementation for quick protective antibody response.

REFERENCES

  1. Bogden, J.D., Bendich, A., Kemp, F.W., Bruening, K.S., Skurnick, J.H., Denny, T., Baker, H. and Louria, D.B. (1994). Daily micronutrient supplements enhance delayed-hypersensitivity skin test response in older people. The American Journal of Clinical Nutrition, 60(3): 437-447.
  2. Chandra, R.K. (1992). Effect of vitamin and trace-element supplementation on immune responses and infection in elderly subjects. The Lancet, 340(8828): 1124–1127.
  3. Fraker, P. and King, L. (1998). Changes in regulation of lymphopoiesis and myelopoiesis in the zinc-deficient mouse. Nutrition Reviews, 56(1): S65-S69.
  4. Haase, H. and Rink, L. (2009). Functional significance of zinc-related signaling pathways in immune cells. Annual Review of Nutrition, 29: 133-152.
  5. Hambidge, K.M. and Walravens, P.A. (1982). Disorders of mineral metabolism. Clinics in Gastroenterology, 11(1): 87-117.
  6. Heaton, P.R., Reed, C.F., Mann, S.J., Ransley, R., Stevenson, J., Charlton, C.J., et al., (2002). Role of dietary antioxidants to protect against DNA damage in adult dogs. The Journal of Nutrition, 132(6): 1720S-1724S.
  7. Heymann, D. (2015). Rabies. Control of Communicable Diseases Manual. (20th Ed.). American Public Health Association, Washington, DC. pp. 497-508. 
  8. Knobel, D.L., Cleaveland, S., Coleman, P.G., Fevre, E.M., Meltzer, M.I., Miranda, M.E.G., et al., (2005). Re-evaluating the burden of rabies in Africa and Asia. Bulletin World Health Organization, 83(5): 360-368.
  9. Koller, L.D., Mulhern, S.A., Frankel, N.C., Steven, M.G. and Williams, J.R. (1987). Immune dysfunction in rats fed a diet deficient in copper. The American Journal of Clinical Nutrition, 45(5): 997-1006.
  10. Maares, M. and Haase, H. (2016). Zinc and immunity: an essential interrelation. Archives of Biochemistry and Biophysics, 611: 58-65.
  11. Mahendra, B.J., Sudarshan, M.K. and Madhusudana, S.N. (1999). Post exposure prophylaxis with purified vero cell rabies vaccine during pregnancy-safety and immunogenicity. The Journal of Communicable Diseases, 31: 229-36. 
  12. Menezes, R. (2008). Rabies in India. Canine Medical Association Journal, 178 (5): 564-566.
  13. Paldurai, A., Singh, R.P., Gupta, P.K., Sharma, B. and Pandey, K.D. (2014). Growth kinetics of rabies virus in BHK-21 cells using fluorescent activated cell sorter (FACS) analysis and a monoclonal antibody based cell-ELISA. Journal of Immunology and Vaccine Technology, 1(1): 103.
  14. Patel, A.C., Upmanyu, V., Ramasamy, S., Gupta, P.K., Singh, R. and Singh, R.P. (2015). Molecular and immunogenic characterization of BHK-21 cell line adapted CVS-11 strain of rabies virus and future prospect in vaccination strategy. Virus Disease, 26(4): 288-296.
  15. Powell, J., Borchers, A.T., Yoshida, S. and Gershwin, M.E. (2000). Evaluation of the immune system in the nutritionally at-risk host. In: Nutrition and Immunology: Principles and Practice. [(Eds) Gershwin M E, German J B and Keen C L]. Humana Press. Totowa, New Jersey. pp. 21-31.
  16. Prasad, A.S. (1998). Zinc in human health: an update. The Journal of Trace Elements in Experimental Medicine, 11(2-3): 63-87.
  17. Thurnham, D.I. and Northrop-Clewes, C.A. (2004). Effects of infection on nutritional and immune status. In: Diet and Human Immune Function [(Eds) Hughes D A, Darlington L G and Bendich A]. Humana Press. Totowa, New Jersey. . pp. 35-64.
  18. Underwood, E.J. and Suttle, N.F. (1999). The Mineral Nutrition of Livestock. (3rd Revised Ed.). CABI Publishing, New York, NY, USA.
  19. WHO. (2013). World Health Organization technical report series, No. 982. WHO expert consultation on rabies. Second report. Geneva: World Health Organization.
  20. Yatoo, M.I., Archana, S., Pankaj, K., Mudasir, B.G., Umesh, D., Mahesh, C.S. and Ricky, J. (2013). Evaluation of serum mineral status and hormone profile in goats and some of their inter-relations. Veterinary World, 6(6): 318-320.
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 4 (april 2018) : 615-618,   Doi: 10.18805/ijar.B-3433

    Effect of mineral supplementation on humoral immunity against rabies vaccine in dog pups

    S
    Sarita Devi
    M
    M.C. Sharma
    R
    R.P. Singh
    U
    U. Dimri
    A
    A.C. Patel
    P
    Pankaj Kumar
    R
    R.D. Singh
    1Division of Medicine, Indian Veterinary Research Institute (IVRI), Izatnagar-243 122, Uttar Pradesh, India.
    Cite article:- Devi Sarita, Sharma M.C., Singh R.P., Dimri U., Patel A.C., Kumar Pankaj, Singh R.D. (2017). Effect of mineral supplementation on humoral immunity against rabies vaccine in dog pups. Indian Journal of Animal Research. 52(4): 615-618. doi: 10.18805/ijar.B-3433.

    ABSTRACT

    The present study was aimed to determine the effect of a specially formulated mineral supplement in shaping the humoral immune response of dog pups in response to anti-rabies vaccination. Mineral supplement was formulated based on plasma analyzed for macro and micro minerals randomly collected from 226 dogs. Twelve mongrel/non-descript dog pups (3 – 6 months) randomly allocated into two groups (n=6) were tested for stimulation of humoral immune system by assessing the antibody titre against rabies vaccine using Rapid Fluorescent Focus Inhibition Test (RFFIT) and in vivo Mouse Neutralization Test (MNT) for antibody detection post supplementation of formulated mineral supplement upto 28 days. Early and higher antibody titre (>1:4096) was recorded on day seven itself in the group of dog pups fed with formulated mineral supplemented as compared to non-supplemented group. Present study indicated that mineral supplementation prior to the anti-rabies vaccination may elicit quick and high level of protective antibodies. The findings may also be important in the event of post bite immunization, in combination with this mineral supplementation for quick protective antibody response.

    REFERENCES

    1. Bogden, J.D., Bendich, A., Kemp, F.W., Bruening, K.S., Skurnick, J.H., Denny, T., Baker, H. and Louria, D.B. (1994). Daily micronutrient supplements enhance delayed-hypersensitivity skin test response in older people. The American Journal of Clinical Nutrition, 60(3): 437-447.
    2. Chandra, R.K. (1992). Effect of vitamin and trace-element supplementation on immune responses and infection in elderly subjects. The Lancet, 340(8828): 1124–1127.
    3. Fraker, P. and King, L. (1998). Changes in regulation of lymphopoiesis and myelopoiesis in the zinc-deficient mouse. Nutrition Reviews, 56(1): S65-S69.
    4. Haase, H. and Rink, L. (2009). Functional significance of zinc-related signaling pathways in immune cells. Annual Review of Nutrition, 29: 133-152.
    5. Hambidge, K.M. and Walravens, P.A. (1982). Disorders of mineral metabolism. Clinics in Gastroenterology, 11(1): 87-117.
    6. Heaton, P.R., Reed, C.F., Mann, S.J., Ransley, R., Stevenson, J., Charlton, C.J., et al., (2002). Role of dietary antioxidants to protect against DNA damage in adult dogs. The Journal of Nutrition, 132(6): 1720S-1724S.
    7. Heymann, D. (2015). Rabies. Control of Communicable Diseases Manual. (20th Ed.). American Public Health Association, Washington, DC. pp. 497-508. 
    8. Knobel, D.L., Cleaveland, S., Coleman, P.G., Fevre, E.M., Meltzer, M.I., Miranda, M.E.G., et al., (2005). Re-evaluating the burden of rabies in Africa and Asia. Bulletin World Health Organization, 83(5): 360-368.
    9. Koller, L.D., Mulhern, S.A., Frankel, N.C., Steven, M.G. and Williams, J.R. (1987). Immune dysfunction in rats fed a diet deficient in copper. The American Journal of Clinical Nutrition, 45(5): 997-1006.
    10. Maares, M. and Haase, H. (2016). Zinc and immunity: an essential interrelation. Archives of Biochemistry and Biophysics, 611: 58-65.
    11. Mahendra, B.J., Sudarshan, M.K. and Madhusudana, S.N. (1999). Post exposure prophylaxis with purified vero cell rabies vaccine during pregnancy-safety and immunogenicity. The Journal of Communicable Diseases, 31: 229-36. 
    12. Menezes, R. (2008). Rabies in India. Canine Medical Association Journal, 178 (5): 564-566.
    13. Paldurai, A., Singh, R.P., Gupta, P.K., Sharma, B. and Pandey, K.D. (2014). Growth kinetics of rabies virus in BHK-21 cells using fluorescent activated cell sorter (FACS) analysis and a monoclonal antibody based cell-ELISA. Journal of Immunology and Vaccine Technology, 1(1): 103.
    14. Patel, A.C., Upmanyu, V., Ramasamy, S., Gupta, P.K., Singh, R. and Singh, R.P. (2015). Molecular and immunogenic characterization of BHK-21 cell line adapted CVS-11 strain of rabies virus and future prospect in vaccination strategy. Virus Disease, 26(4): 288-296.
    15. Powell, J., Borchers, A.T., Yoshida, S. and Gershwin, M.E. (2000). Evaluation of the immune system in the nutritionally at-risk host. In: Nutrition and Immunology: Principles and Practice. [(Eds) Gershwin M E, German J B and Keen C L]. Humana Press. Totowa, New Jersey. pp. 21-31.
    16. Prasad, A.S. (1998). Zinc in human health: an update. The Journal of Trace Elements in Experimental Medicine, 11(2-3): 63-87.
    17. Thurnham, D.I. and Northrop-Clewes, C.A. (2004). Effects of infection on nutritional and immune status. In: Diet and Human Immune Function [(Eds) Hughes D A, Darlington L G and Bendich A]. Humana Press. Totowa, New Jersey. . pp. 35-64.
    18. Underwood, E.J. and Suttle, N.F. (1999). The Mineral Nutrition of Livestock. (3rd Revised Ed.). CABI Publishing, New York, NY, USA.
    19. WHO. (2013). World Health Organization technical report series, No. 982. WHO expert consultation on rabies. Second report. Geneva: World Health Organization.
    20. Yatoo, M.I., Archana, S., Pankaj, K., Mudasir, B.G., Umesh, D., Mahesh, C.S. and Ricky, J. (2013). Evaluation of serum mineral status and hormone profile in goats and some of their inter-relations. Veterinary World, 6(6): 318-320.
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