Sequence characterization and SNP identification  of TNP1 gene in Indian cattle breeds

DOI: 10.18805/ijar.v0iOF.8492    | Article Id: B-3424 | Page : 1680-1683
Citation :- Sequence characterization and SNP identification of TNP1 gene in Indiancattle breeds .Indian Journal Of Animal Research.2018.(52):1680-1683

Ashish Ranjan, K. N. Raja, Ranjana Sinha, I.Ganguly, I.D Gupta, M Bhakat and T. K. Mohanty
Address :

Division of Animal Genetics and Breeding, ICAR -National Dairy Research Institute, Karnal-132 001, Haryana, India.

Submitted Date : 28-04-2017
Accepted Date : 23-06-2017


Present study was conducted on 50 bulls and 40 male calves of Sahiwal, Tharparkar and Karan Fries cattle maintained at ABRC and LRC, NDRI Karnal (Haryana) to characterize and identify genetic polymorphisms in TNP1 gene. A total of 1568 bp region of TNP-1 gene includes 490 bp of promoter region and two exon and one intron was sequenced and characterized in Bos indicus cattle breeds which are widely distributed in Indian sub-continent. Four sets of primers for TNP1 gene on the basis of Bos Taurus sequence (Acc. No- BK_006511) were designed using Primer3 software and PCR products of 487, 450, 455 and 250 bp were obtained. Amplicons were custom sequenced and subjected to Clustal W analysis which showed no nucleotide changes in coding region and non coding region in Indian cattle breeds as compared to Bos taurus. The 490 bp of promoter region was subjected to transcription factor binding site. Three TATA boxes and two CAAT boxes were identified in the studied fragment. Analysis of SNP was performed using restriction fragment length polymorphism (PCR-RFLP), to detect nucleotide changes in the sequence as reported (g.528G>A, SS1388116558) in Chinese Holstein breed. No polymorphisms were found for tested SNP. Only one genotype GG indicates the absence of variability in the sampled population.


CAAT boxes Nucleotide sequences Polymorphism TATA boxes TNP1 gene.


  1. Akama, K., Sato, H., Hasegawa, S., Shimada, I. and Nakano, M. (1998). Transition protein-1 from boar late spermatid nuclei having DNA-melting activity is a dimeric protein. Biochem. Mol Biol Int., 44: 315–323.
  2. Braundmeier, A.G. and Miller, D.G. (2001).The search is on: Finding Accurate molecular markers of male fertility. J Dairy Sci., 84: 1915-1925.
  3. Caron, N., Veilleux, S. and Boissonneault, G. (2001). Stimulation of DNA repair by the spermatidal TP1 protein. Mol Reprod Dev., 58: 437–443.
  4. Forestra, C., Zorzi, M., Rossato, M. and Varotto, A. (1992). Sperm nuclear instability and staining with aniline blue: abnormal persistence of histones in spermatozoa of infertile men. Int. J. Androl. 15: 330-337.
  5. Kierszenbaum, A.L. (2001). Transition nuclear proteins during spermiogenesis: unrepaired DNA breaks not allowed. Mol. Reprod. Develop. 58: 357–358.
  6. Kim, Y., Kremling, H., Tessmann, D. and Engel, W. (2009). Nucleotide sequence and exon-intron structure of the bovine transition protein 1 gene. DNA Seq. 3(2): 123-125.
  7. Kremling, H., Luerssen, H., Adham, I.M., Klemm, U., Tsaousidou, S., Engel, W. (1989). Nucleotide sequences and expression of cDNA clones for boar and bull transition protein 1 and its evolutionary conservation in mammals. Differentiation, 40: 184-190.
  8. Lucy, M.C. (2001). Reproductive loss in high-producing dairy cattle: where will it end. J. Dairy Sci. pp 1277–1293.
  9. Mukhopadhyay, C.S., Gupta, A.K., Yadav, B.R., Khate, K., Raina, V.S., Mohanty T. K. and Dubey. P.P. (2010). Subfertility in males: an important cause of bull disposal in bovines. AsianAust. J Anim Sci., 23: 450-455.
  10. Mylonis I, Drosou V, Brancorsini S, Nikolakaki E, Sassone-Corsi P, Giannakouros T. (2005). Temporal association of protamine 1 with the inner nuclear membrane protein lamin B receptor during spermiogenesis. J Biol Chem., 279: 11626-31.
  11. Panigrahi, S.K. and Yadav, B.R. (2009). Polymorphism in TNP-1gene of Murrah buffalo bulls. African Journal of Biotechnology. 9(43): 7224-7229.
  12. Rendel , J.M. and Robertsson, A. (1950). Estimation of genetic gain in milk yield by selection in a closed herd of dairy cattle. J.Genet.50(1):1-8.
  13. Sambrook, J. and Russell, W. D. (2001). Molecular cloning.A laboratory manual 1st volume. Cold Spring Harbor Laboratory Press, New York: pp. 1.32-1.34 and 8.14.
  14. Singh, J. and Rao. M.R.S. (1988). Interaction of rat testis protein, TP, with nucleosome core particle. Biochem. Int. 17: 701–710.
  15. Zhang, S., Zhang, Y., Yang, C., Zhang, W., Ju, Z., Wang, X., Jiang, Q., Sun,Y., J Huang, J., Zhong, J. and Wang, C. (2015). TNP1 functional SNPs in bta-miR-532 and bta-miR-204 target sites are associated with semen quality traits in Chinese Holstein bulls. Biolreprod. 10: 1095

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