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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 11 (november 2018) : 1654-1655,   Doi: 10.18805/ijar.B-3415

Detection of mammalian-like group a rotavirus in diarrhoeic poultry using RNA-PAGE In Kerala, India

R
Rinsha Balan
M
M. Mini
P
P.M Priya
S
Siju Joseph
S
Surya Sankar
1Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala-680 651, India
Cite article:- Balan Rinsha, Mini M., Priya P.M, Joseph Siju, Sankar Surya (2018). Detection of mammalian-like group a rotavirus in diarrhoeic poultry using RNA-PAGE In Kerala, India. Indian Journal of Animal Research. 52(11): 1654-1655. doi: 10.18805/ijar.B-3415.

ABSTRACT

The present work was conducted to determine the prevalence of rotavirus infection among poultry birds having diarrhoea. A total of 143 faecal samples were collected from different parts of Kerala and screened for the presence of rotavirus using RNA polyacrylamide gel electrophoresis (RNA-PAGE). Out of 143 samples, 5 (3.49%) were found to be positive in RNA PAGE with a migration pattern 4:2:3:2 of a mammalian-like electropherogroup A rotavirus. The study records the first evidence of rota virus detection from avian species in Kerala.

REFERENCES

  1. Chomczynski, P. (1993). A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechology 15: 532-537.
  2. Kalica, A.R. and Wyatt, R.G., Kapikian, A.Z. (1978). Detection of differences among human and animal rotaviruses using analysis of viral RNA. Journal of American Veterinary Medical Association 173: 531-537.
  3. Kattoor, J.J., Sharma, K, Kumar, N., Batra, M., Jindal, N., Yadav, A.S., Malik, Y.S. (2013). Distribution Frequency of Avian Rotaviruses in India, 2011–2013. Advances in Animal and Veterinary Sciences 1 (5): 148 – 150.
  4. McNulty, M.S., Allan, G.M., Stuart, J.C. (1978). Rotavirus infection in avian species. Veterinary Record 103:319-320.
  5. Suresh, T., Rai, R.B., Wani, M.Y., Damodaran, T., Dhama, K. (2014). Detection of bovine rotavirus in neonatal calf diarrhoea by ELISA, FAT and Transmission Electron Microscopy. International Journal of Current Research 5: 1935-1939.
  6. Wani, S.A., Bhat, S.M., Ishaq, M.A., Ashrafi, A.S., Buchhand, M. (2003). Detection of a mammalian-like group A rotavirus in diarrhoeic chicken. Veterinary Microbiolology 94: 13-18.
  7. Wani, S.A., Bhat, M.A., Ishaq, S.M., Ashrafi, M.A. (2004). Determination of bovine rotavirus G genotypes in Kashmir, India. Scientific and Technical Review 23(3): 931-936.
  8. Yason, C.V., Schat, K.A. (1985). Isolation and characterization of avian rotaviruses. Avian Disease 29: 499-508
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 11 (november 2018) : 1654-1655,   Doi: 10.18805/ijar.B-3415

    Detection of mammalian-like group a rotavirus in diarrhoeic poultry using RNA-PAGE In Kerala, India

    R
    Rinsha Balan
    M
    M. Mini
    P
    P.M Priya
    S
    Siju Joseph
    S
    Surya Sankar
    1Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala-680 651, India
    Cite article:- Balan Rinsha, Mini M., Priya P.M, Joseph Siju, Sankar Surya (2018). Detection of mammalian-like group a rotavirus in diarrhoeic poultry using RNA-PAGE In Kerala, India. Indian Journal of Animal Research. 52(11): 1654-1655. doi: 10.18805/ijar.B-3415.

    ABSTRACT

    The present work was conducted to determine the prevalence of rotavirus infection among poultry birds having diarrhoea. A total of 143 faecal samples were collected from different parts of Kerala and screened for the presence of rotavirus using RNA polyacrylamide gel electrophoresis (RNA-PAGE). Out of 143 samples, 5 (3.49%) were found to be positive in RNA PAGE with a migration pattern 4:2:3:2 of a mammalian-like electropherogroup A rotavirus. The study records the first evidence of rota virus detection from avian species in Kerala.

    REFERENCES

    1. Chomczynski, P. (1993). A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechology 15: 532-537.
    2. Kalica, A.R. and Wyatt, R.G., Kapikian, A.Z. (1978). Detection of differences among human and animal rotaviruses using analysis of viral RNA. Journal of American Veterinary Medical Association 173: 531-537.
    3. Kattoor, J.J., Sharma, K, Kumar, N., Batra, M., Jindal, N., Yadav, A.S., Malik, Y.S. (2013). Distribution Frequency of Avian Rotaviruses in India, 2011–2013. Advances in Animal and Veterinary Sciences 1 (5): 148 – 150.
    4. McNulty, M.S., Allan, G.M., Stuart, J.C. (1978). Rotavirus infection in avian species. Veterinary Record 103:319-320.
    5. Suresh, T., Rai, R.B., Wani, M.Y., Damodaran, T., Dhama, K. (2014). Detection of bovine rotavirus in neonatal calf diarrhoea by ELISA, FAT and Transmission Electron Microscopy. International Journal of Current Research 5: 1935-1939.
    6. Wani, S.A., Bhat, S.M., Ishaq, M.A., Ashrafi, A.S., Buchhand, M. (2003). Detection of a mammalian-like group A rotavirus in diarrhoeic chicken. Veterinary Microbiolology 94: 13-18.
    7. Wani, S.A., Bhat, M.A., Ishaq, S.M., Ashrafi, M.A. (2004). Determination of bovine rotavirus G genotypes in Kashmir, India. Scientific and Technical Review 23(3): 931-936.
    8. Yason, C.V., Schat, K.A. (1985). Isolation and characterization of avian rotaviruses. Avian Disease 29: 499-508
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