Research Article

volume 52 issue 11 (november 2018) : 1654-1655, Doi: 10.18805/ijar.B-3415

Detection of mammalian-like group a rotavirus in diarrhoeic poultry using RNA-PAGE In Kerala, India

R

Rinsha Balan

M

M. Mini

P

P.M Priya

S

Siju Joseph

S

Surya Sankar

Affiliations

¹Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala-680 651, India

Submitted29-03-2017|
Accepted12-12-2017|
First Online 19-02-2018|
doi 10.18805/ijar.B-3415

Cite article:- Balan Rinsha, Mini M., Priya P.M, Joseph Siju, Sankar Surya (2018). Detection of mammalian-like group a rotavirus in diarrhoeic poultry using RNA-PAGE In Kerala, India. Indian Journal of Animal Research. 52(11): 1654-1655. doi: 10.18805/ijar.B-3415.

ABSTRACT

The present work was conducted to determine the prevalence of rotavirus infection among poultry birds having diarrhoea. A total of 143 faecal samples were collected from different parts of Kerala and screened for the presence of rotavirus using RNA polyacrylamide gel electrophoresis (RNA-PAGE). Out of 143 samples, 5 (3.49%) were found to be positive in RNA PAGE with a migration pattern 4:2:3:2 of a mammalian-like electropherogroup A rotavirus. The study records the first evidence of rota virus detection from avian species in Kerala.

KEYWORDS

REFERENCES

Chomczynski, P. (1993). A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechology 15: 532-537.
Kalica, A.R. and Wyatt, R.G., Kapikian, A.Z. (1978). Detection of differences among human and animal rotaviruses using analysis of viral RNA. Journal of American Veterinary Medical Association 173: 531-537.
Kattoor, J.J., Sharma, K, Kumar, N., Batra, M., Jindal, N., Yadav, A.S., Malik, Y.S. (2013). Distribution Frequency of Avian Rotaviruses in India, 2011–2013. Advances in Animal and Veterinary Sciences 1 (5): 148 – 150.
McNulty, M.S., Allan, G.M., Stuart, J.C. (1978). Rotavirus infection in avian species. Veterinary Record 103:319-320.
Suresh, T., Rai, R.B., Wani, M.Y., Damodaran, T., Dhama, K. (2014). Detection of bovine rotavirus in neonatal calf diarrhoea by ELISA, FAT and Transmission Electron Microscopy. International Journal of Current Research 5: 1935-1939.
Wani, S.A., Bhat, S.M., Ishaq, M.A., Ashrafi, A.S., Buchhand, M. (2003). Detection of a mammalian-like group A rotavirus in diarrhoeic chicken. Veterinary Microbiolology 94: 13-18.
Wani, S.A., Bhat, M.A., Ishaq, S.M., Ashrafi, M.A. (2004). Determination of bovine rotavirus G genotypes in Kashmir, India. Scientific and Technical Review 23(3): 931-936.
Yason, C.V., Schat, K.A. (1985). Isolation and characterization of avian rotaviruses. Avian Disease 29: 499-508

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This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Research Article

Detection of mammalian-like group a rotavirus in diarrhoeic poultry using RNA-PAGE In Kerala, India

ABSTRACT

KEYWORDS

REFERENCES

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