Occurrence of Arcobacter species in animal faeces, foods of animal origin and humans in Andhra Pradesh, India

DOI: 10.18805/ijar.B-3402    | Article Id: B-3402 | Page : 1649-1653
Citation :- Occurrence of Arcobacter species in animal faeces, foods of animal origin and humans in Andhra Pradesh, India.Indian Journal Of Animal Research.2018.(52):1649-1653
M. Soma Sekhar, S.R. Tumati, B.K. Chinnam, V.S. Kothapalli and N. Mohammad Sharif somasekharmadupuru@gmail.com
Address : Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University (SVVU), Tirupati – 517 502, India.
Submitted Date : 6-03-2017
Accepted Date : 13-07-2017

Abstract

A total of n=510 samples comprising of faecal swabs from livestock (n=245), foods of animal origin (n=180) and human stools (n=85) were examined to detect Arcobacter species employing cultural and PCR methods. Overall, 11.7% of samples were found positive for Arcobacter species by genus-specific PCR. On screening faecal samples of livestocks, higher positivity was observed in pigs (23.3%), followed by chicken (16.6%), turkey (15.0%), cattle (10.0%), duck (10.0%) and sheep (6.6%). With regard to foods of animal origin, higher positivity was observed in pork samples (15.0%), followed by chicken (12.5%), milk (10.0%) and mutton (7.5%). In humans, the stool samples of farm workers revealed high positivity (13.3%) followed by veterinary students (8.0%) and diarrhoeic humans (6.66%). Using cultural method, a total of 41 (8.03%) Arcobacter isolates were recovered. The multiplex-PCR assay enabled detection of Arcobacter butzleri (16/41), Arcobacter cryaerophilus (13/41) and Arcobacter skirrowii (12/41). The results signify the occurrence of Arcobacter species in animal faeces, foods of animal origin and humans in Andhra Pradesh, India.

Keywords

Arcobacter Cultural isolation Emerging food borne pathogen Genus-specific PCR multiplex PCR.

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