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Research Article

volume 52 issue 11 (november 2018) : 1548-1551,   Doi: 10.18805/ijar.B-3400

Association of single nucleotide polymorphisms of PPP1R11 gene with conception rate in Karan Fries bull

S
Shabahat Mumtaz
A
Anupama Mukherjee
A
Abdul Rahim
K
Kaiser Parveen
P
Pooja Joshi
A
Alok Kumar Yadav
A
A.K. Gupta
1Animal Genetics & Breeding Division, ICAR-National Dairy Research Institute, Karnal-132001,Haryana, India.
Cite article:- Mumtaz Shabahat, Mukherjee Anupama, Rahim Abdul, Parveen Kaiser, Joshi Pooja, Yadav Kumar Alok, Gupta A.K. (2017). Association of single nucleotide polymorphisms of PPP1R11 gene with conception rate in Karan Fries bull. Indian Journal of Animal Research. 52(11): 1548-1551. doi: 10.18805/ijar.B-3400.

ABSTRACT

Bull fertility is an important component of reproductive efficiency in dairy cattle. PPP1R11 is a potent candidate gene for bull fertility being involved in the pathway of spermatogenesis. Present investigation was carried out in twenty Karan Fries (KF) bull to identify SNPs of PPP1R11 gene and their association with conception rate. Genomic DNA was extracted from frozen semen straws by phenol-chloroform method. Primer pair targeted to exonic II region of PPP1R11 gene was designed using Primer 3.1 software. PCR reactions were carried out in thermal cycler after optimizing. The PCR product was checked on 1.5% horizontal agarose gel electrophoresis to verify the amplification of target region. Samples were sequenced and SNPs were identified using multiple alignment programme with respect to reference sequence and visual inspection of chromatograms. Sequencing results revealed only two SNPs [G28708846A, T28708860C] at exonic II region of PPP1R11 gene in KF bulls. Each bull was genotyped with respect to each of the two identified SNPs. Association of SNP genotype with conception rate was analyzed using least square ANOVA taking genotypes as fixed effect. The result indicated that, only one SNP [T28708860C] had significant (p<0.01) effect on conception rate. The bulls with TT genotype revealed significantly (p<0.01) highest conception rate (53.20±2.96%) followed by bulls with TC (43.12±2.39%) and the lowest in bulls with CC (34.59± 2.28%) genotypes. The results of present finding suggested that association of SNPs with conception rate after validation on large population might be useful in marker assisted selection for selection of bull with high fertility in future.

REFERENCES

  1. 19th Livestock Censuses. (2012). Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture, Govt. of India, New Delhi.
  2. Cheng, L., Pilder, S., Nairn, A. C., Ramdas, S. and Vijayaraghavan, S. (2009). PP1c2 and PPP1R11 are parts of a multimeric complex in developing testicular germ cells in which their steady state levels are reciprocally related. PLoS ONE, 4: 4861. 
  3. Dayal, S., Kumari, R., Dey, A. and Kumar B (2016) PCR-SSCP of growth hormone gene and its association with body weight in Black Bengal goat. Indian J. Anim. Sci., 86: 1283-1286. 
  4. Han, Y. B., Feng, H. L., Cheng, C. K., Lam, P. M., Wang, C. C. and Haines, C. J. (2007). Expression of a novel T-complex testis expressed 5 (Tctex5) in mouse testis, epididymis, and spermatozoa. Mol. Reprod. Dev., 74:1132-1140.
  5. Han, Y., Song, X. X., Feng, H. L., Cheung, C. K., Lam, P. M., Wang, C. C., and Haines, C. J. (2008). Mutations of t-complex testis expressed gene 5 transcripts in the testis of sterile t-haplotype mutant mouse. Asian J. Androl., 10: 219–226. 
  6. Harvey, W. R. (1990). User’s guide for LSMLMW, mixed model least squares and maximum likelihood computer programme. Ohio State University (Mimeograph).
  7. Khatib, H. (2014). Single nucleotide polymorphisms associated with bull fertility. Patent publication number US20150307943A1. 
  8. Kramer, C. Y. (1957). Extension of multiple range tests to group correlated adjusted means. Biometrics, 13: 13-18.
  9. Kuhn, M. T., Hutchison, J. L. and Norman, H. D. (2008). Modelling nuisance variables for prediction of service sire fertility. J. Dairy Sci., 91: 2823–2835.
  10. Kumar, A., Vineeth, M. R., Sinha R., Singh, R. K., Thakur, A. and Gupta S. K. (2016). Current status, scope and constraints of sexed semen-An Indian perspective. Agricult. Rev., 37 (3): 240-244.
  11. Li G., Penagaricano F., Weigel K. A., Zhang, Y., Rosa, G. and H. Khatib (2012). Comparative genomics between fly, mouse, and cattle identifies genes associated with sire conception rate. J. Dairy Sci., 95: 6122–6129. 
  12. Lucy, M. C. (2001). Reproductive loss in high- producing dairy cattle: Where will it end. J. Dairy Sci., 84: 1277-1293.
  13. MacLeod G., Shang P., Booth G. T., Mastropaolo. L. A., Manafpoursakha N., Vogl A. W., and Varmuza S. (2014). PPP1CC2 can form a kinase/phosphatase complex with the testis-specific proteins TSSK1 and TSKS in the mouse testis. Reprod. 147:1-12.
  14. Mahmoud, K.G.M. and Nawito, M. F. (2012). Molecular markers for fertility in farm animals. Iranian J. Appl. Anim. Sci., 2: 203-222.
  15. Mukhopadhyay, C. S., Gupta, A. K., Yadav, B. R., Khate, K., Raina, V. S., Mohanty, T. K. and Dubey, P. P. (2010). Subfertility in males: an important cause of bull disposal in bovines. Asian Aust. J Anim Sci., 23:450-55.
  16. Naha, B. C., Chakravarty, A. K., Mir, M. A. and Gupta, A. K. (2015). Evaluation of sire conception rate in Sahiwal breeding bulls. Indian Vet. J., 92: 70 – 71.
  17. Panmei A., Gupta, A. K. Shivahre, P. R., Bhakat, M. and Dash S. K. (2016). Culling pattern of Karan Fries (crossbred) males in an organised herd. Indian J. Anim. Res., 50: 1005-1008.
  18. Sambrook, J. and Russel, D. W. (2001). Molecular cloning. A Laboratory Manual 3rd ed. 1 Cold Harbour Spring Laboratory, Press, Cold Spring, NewYork: 64.
  19. Singh, M. and Pant, H. C. (1999). Effect of post- thawed interval to A. I. on conception rate. Indian Vet. J., 76: 67-68.
  20. Vineeth, M. R. (2015). Identification of SNPs in PROP1 gene and their association with sire conception rate in Sahiwal and Karan Fries bulls. M.V.Sc. Thesis submitted to ICAR-NDRI, Karnal, India. 
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    Research Article

    volume 52 issue 11 (november 2018) : 1548-1551,   Doi: 10.18805/ijar.B-3400

    Association of single nucleotide polymorphisms of PPP1R11 gene with conception rate in Karan Fries bull

    S
    Shabahat Mumtaz
    A
    Anupama Mukherjee
    A
    Abdul Rahim
    K
    Kaiser Parveen
    P
    Pooja Joshi
    A
    Alok Kumar Yadav
    A
    A.K. Gupta
    1Animal Genetics & Breeding Division, ICAR-National Dairy Research Institute, Karnal-132001,Haryana, India.
    Cite article:- Mumtaz Shabahat, Mukherjee Anupama, Rahim Abdul, Parveen Kaiser, Joshi Pooja, Yadav Kumar Alok, Gupta A.K. (2017). Association of single nucleotide polymorphisms of PPP1R11 gene with conception rate in Karan Fries bull. Indian Journal of Animal Research. 52(11): 1548-1551. doi: 10.18805/ijar.B-3400.

    ABSTRACT

    Bull fertility is an important component of reproductive efficiency in dairy cattle. PPP1R11 is a potent candidate gene for bull fertility being involved in the pathway of spermatogenesis. Present investigation was carried out in twenty Karan Fries (KF) bull to identify SNPs of PPP1R11 gene and their association with conception rate. Genomic DNA was extracted from frozen semen straws by phenol-chloroform method. Primer pair targeted to exonic II region of PPP1R11 gene was designed using Primer 3.1 software. PCR reactions were carried out in thermal cycler after optimizing. The PCR product was checked on 1.5% horizontal agarose gel electrophoresis to verify the amplification of target region. Samples were sequenced and SNPs were identified using multiple alignment programme with respect to reference sequence and visual inspection of chromatograms. Sequencing results revealed only two SNPs [G28708846A, T28708860C] at exonic II region of PPP1R11 gene in KF bulls. Each bull was genotyped with respect to each of the two identified SNPs. Association of SNP genotype with conception rate was analyzed using least square ANOVA taking genotypes as fixed effect. The result indicated that, only one SNP [T28708860C] had significant (p<0.01) effect on conception rate. The bulls with TT genotype revealed significantly (p<0.01) highest conception rate (53.20±2.96%) followed by bulls with TC (43.12±2.39%) and the lowest in bulls with CC (34.59± 2.28%) genotypes. The results of present finding suggested that association of SNPs with conception rate after validation on large population might be useful in marker assisted selection for selection of bull with high fertility in future.

    REFERENCES

    1. 19th Livestock Censuses. (2012). Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture, Govt. of India, New Delhi.
    2. Cheng, L., Pilder, S., Nairn, A. C., Ramdas, S. and Vijayaraghavan, S. (2009). PP1c2 and PPP1R11 are parts of a multimeric complex in developing testicular germ cells in which their steady state levels are reciprocally related. PLoS ONE, 4: 4861. 
    3. Dayal, S., Kumari, R., Dey, A. and Kumar B (2016) PCR-SSCP of growth hormone gene and its association with body weight in Black Bengal goat. Indian J. Anim. Sci., 86: 1283-1286. 
    4. Han, Y. B., Feng, H. L., Cheng, C. K., Lam, P. M., Wang, C. C. and Haines, C. J. (2007). Expression of a novel T-complex testis expressed 5 (Tctex5) in mouse testis, epididymis, and spermatozoa. Mol. Reprod. Dev., 74:1132-1140.
    5. Han, Y., Song, X. X., Feng, H. L., Cheung, C. K., Lam, P. M., Wang, C. C., and Haines, C. J. (2008). Mutations of t-complex testis expressed gene 5 transcripts in the testis of sterile t-haplotype mutant mouse. Asian J. Androl., 10: 219–226. 
    6. Harvey, W. R. (1990). User’s guide for LSMLMW, mixed model least squares and maximum likelihood computer programme. Ohio State University (Mimeograph).
    7. Khatib, H. (2014). Single nucleotide polymorphisms associated with bull fertility. Patent publication number US20150307943A1. 
    8. Kramer, C. Y. (1957). Extension of multiple range tests to group correlated adjusted means. Biometrics, 13: 13-18.
    9. Kuhn, M. T., Hutchison, J. L. and Norman, H. D. (2008). Modelling nuisance variables for prediction of service sire fertility. J. Dairy Sci., 91: 2823–2835.
    10. Kumar, A., Vineeth, M. R., Sinha R., Singh, R. K., Thakur, A. and Gupta S. K. (2016). Current status, scope and constraints of sexed semen-An Indian perspective. Agricult. Rev., 37 (3): 240-244.
    11. Li G., Penagaricano F., Weigel K. A., Zhang, Y., Rosa, G. and H. Khatib (2012). Comparative genomics between fly, mouse, and cattle identifies genes associated with sire conception rate. J. Dairy Sci., 95: 6122–6129. 
    12. Lucy, M. C. (2001). Reproductive loss in high- producing dairy cattle: Where will it end. J. Dairy Sci., 84: 1277-1293.
    13. MacLeod G., Shang P., Booth G. T., Mastropaolo. L. A., Manafpoursakha N., Vogl A. W., and Varmuza S. (2014). PPP1CC2 can form a kinase/phosphatase complex with the testis-specific proteins TSSK1 and TSKS in the mouse testis. Reprod. 147:1-12.
    14. Mahmoud, K.G.M. and Nawito, M. F. (2012). Molecular markers for fertility in farm animals. Iranian J. Appl. Anim. Sci., 2: 203-222.
    15. Mukhopadhyay, C. S., Gupta, A. K., Yadav, B. R., Khate, K., Raina, V. S., Mohanty, T. K. and Dubey, P. P. (2010). Subfertility in males: an important cause of bull disposal in bovines. Asian Aust. J Anim Sci., 23:450-55.
    16. Naha, B. C., Chakravarty, A. K., Mir, M. A. and Gupta, A. K. (2015). Evaluation of sire conception rate in Sahiwal breeding bulls. Indian Vet. J., 92: 70 – 71.
    17. Panmei A., Gupta, A. K. Shivahre, P. R., Bhakat, M. and Dash S. K. (2016). Culling pattern of Karan Fries (crossbred) males in an organised herd. Indian J. Anim. Res., 50: 1005-1008.
    18. Sambrook, J. and Russel, D. W. (2001). Molecular cloning. A Laboratory Manual 3rd ed. 1 Cold Harbour Spring Laboratory, Press, Cold Spring, NewYork: 64.
    19. Singh, M. and Pant, H. C. (1999). Effect of post- thawed interval to A. I. on conception rate. Indian Vet. J., 76: 67-68.
    20. Vineeth, M. R. (2015). Identification of SNPs in PROP1 gene and their association with sire conception rate in Sahiwal and Karan Fries bulls. M.V.Sc. Thesis submitted to ICAR-NDRI, Karnal, India. 
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