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Current IssueEditorial BoardOnline First ArticlesArchive

Short Communication

volume 52 issue 10 (october 2018) : 1503-1505,   Doi: 10.18805/ijar.B-3388

Detection of anti-Salmonella antibodies in food animals of Nagpur region by In-house ELISA

D
D.G. Kalambhe
N
N.N. Zade
S
S.P. Chaudhari
S
S.V. Shinde
W
W.A. Khan
V
V.N. Hirde
1Department of Veterinary Public Health, Nagpur Veterinary College, Nagpur-440 006, Maharashtra, India.
Cite article:- Kalambhe D.G., Zade N.N., Chaudhari S.P., Shinde S.V., Khan W.A., Hirde V.N. (2018). Detection of anti-Salmonella antibodies in food animals of Nagpur region by In-house ELISA. Indian Journal of Animal Research. 52(10): 1503-1505. doi: 10.18805/ijar.B-3388.

ABSTRACT

Salmonella is a widespread enteric pathogen of many animal species, including mammals, birds, insects, reptiles and humans. It is an opportunistic bacterium, infecting immunosuppressed animal and takes over lead in the absence of other competing gut bacteria. Salmonella Typhimurium  DT104, is of particular public health concern. In the present study sero-survey based on in-house enzyme-linked immunosorbent assays (ELISAs) have been attempted to study prevalence of anti-Salmonella antibodies among food animals by employing whole cell (WC) and lipopolysachharide (LPS) antigens. A total of 200 sera samples comprising 50 each from slaughter male cattle, buffaloes, goats and pigs were included in a study. WC-ELISA recorded 24% (cattle), 6% (buffalo), 68% (goat) and 74% (pigs) seropositivity for Salmonella whereas LPS-ELISA estimated seroprevalence of 8%, 0%, 56% and 40% among cattle, buffaloes, goats and pigs respectively. Though the results of ELISAs based on two antigens are not parallel, but the fact of presence of anti-Salmonella antibodies among food animals cannot be denied which is important from public health point of view. More comprehensive studies on livestock salmonellosis are required for further analysis of the bacterial reservoir for human infection. The usefulness of these two antigens as the diagnostic markers for detecting anti-Salmonella antibodies requires more study.

REFERENCES

  1. Apicella, MA (1996) Isolation and characterization of lipopolysaccharides. [F. Deleo and M. Otto.] Human Press, Totowa, pp. 3-13.
  2. Singh BR, Verma JC, Singh KP and Singh YP (2009). Sero-prevalence of Salmonella infection in animals in North India. Haryana Veterinary, 48: 43-46.
  3. Baker MG, Thorns C, Lopez LD, Garrett NK and Nikol CM (2007). A recurring salmonellosis epidemic in New Zealand linked to contact with sheep. Epidemiology and Infection, 135 :76-83.
  4. Camilla W, Jauho ES, Stryhn H, Andresen LO, Thaulov K, Boas U, Jakobsen MH and Heegaard PHM (2000). Evaluation of a noval enzyme linked immunosorbent assay for detection of antibodies against Salmonella, employing a stable coating of lipopolysaccharide derived antigen covalently attached to polystyrene microwells. Journal of Veterinary Diagnosis and Investigations, 12: 130-135. 
  5. Chandra M, Singh R, Shankar H, Agarwal M, Agarwal RK, Sharma G and Babu N (2007). Seroprevalence of Salmonella among goats slaughtered for chevon in Bareilly (Northern India). Preventive Veterinary Medicine, 80: 1-8.
  6. Munford RS, Hall CL and Rick PD (1980). Size heterogeneity of Salmonella Typhimurium lipopolysaccharides in outer-membranes and culture supernatant membrane fragments. Journal of Bacteriology, 14: 630-640.
  7. Rezania S, Amirmozaffari N, Tabarraei B, Jeddi-Tehrani M, Zarei O, Alizadeh R, Masjedian F and Zarnani AH (2011). Extraction, purification and characterization of lipopolysaccharide from Escherichia coli and Salmonella typhi . Avicenna Journal of Medical Biotechnology, 3(1):3-9.
  8. Sachan N, Nautiyal B, Agarwal RK, Singh VP and Verma AK (2013). Sero-epidemiological pattern of salmonellosis in animals of Rohilkhand region, Uttar Pradesh, India Journal of Animal Health and Production, 1 (1): 6–9.
  9. Saxena SN, Kumari N, Saini SS, Soni DV, Pahwa RK, Jayasheela M (1989). Surveillance of Salmonella in India for drug Resistance. Indian Journal of Medical Science, 43: 145-150.
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Indian Journal of Animal Research
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    Short Communication

    volume 52 issue 10 (october 2018) : 1503-1505,   Doi: 10.18805/ijar.B-3388

    Detection of anti-Salmonella antibodies in food animals of Nagpur region by In-house ELISA

    D
    D.G. Kalambhe
    N
    N.N. Zade
    S
    S.P. Chaudhari
    S
    S.V. Shinde
    W
    W.A. Khan
    V
    V.N. Hirde
    1Department of Veterinary Public Health, Nagpur Veterinary College, Nagpur-440 006, Maharashtra, India.
    Cite article:- Kalambhe D.G., Zade N.N., Chaudhari S.P., Shinde S.V., Khan W.A., Hirde V.N. (2018). Detection of anti-Salmonella antibodies in food animals of Nagpur region by In-house ELISA. Indian Journal of Animal Research. 52(10): 1503-1505. doi: 10.18805/ijar.B-3388.

    ABSTRACT

    Salmonella is a widespread enteric pathogen of many animal species, including mammals, birds, insects, reptiles and humans. It is an opportunistic bacterium, infecting immunosuppressed animal and takes over lead in the absence of other competing gut bacteria. Salmonella Typhimurium  DT104, is of particular public health concern. In the present study sero-survey based on in-house enzyme-linked immunosorbent assays (ELISAs) have been attempted to study prevalence of anti-Salmonella antibodies among food animals by employing whole cell (WC) and lipopolysachharide (LPS) antigens. A total of 200 sera samples comprising 50 each from slaughter male cattle, buffaloes, goats and pigs were included in a study. WC-ELISA recorded 24% (cattle), 6% (buffalo), 68% (goat) and 74% (pigs) seropositivity for Salmonella whereas LPS-ELISA estimated seroprevalence of 8%, 0%, 56% and 40% among cattle, buffaloes, goats and pigs respectively. Though the results of ELISAs based on two antigens are not parallel, but the fact of presence of anti-Salmonella antibodies among food animals cannot be denied which is important from public health point of view. More comprehensive studies on livestock salmonellosis are required for further analysis of the bacterial reservoir for human infection. The usefulness of these two antigens as the diagnostic markers for detecting anti-Salmonella antibodies requires more study.

    REFERENCES

    1. Apicella, MA (1996) Isolation and characterization of lipopolysaccharides. [F. Deleo and M. Otto.] Human Press, Totowa, pp. 3-13.
    2. Singh BR, Verma JC, Singh KP and Singh YP (2009). Sero-prevalence of Salmonella infection in animals in North India. Haryana Veterinary, 48: 43-46.
    3. Baker MG, Thorns C, Lopez LD, Garrett NK and Nikol CM (2007). A recurring salmonellosis epidemic in New Zealand linked to contact with sheep. Epidemiology and Infection, 135 :76-83.
    4. Camilla W, Jauho ES, Stryhn H, Andresen LO, Thaulov K, Boas U, Jakobsen MH and Heegaard PHM (2000). Evaluation of a noval enzyme linked immunosorbent assay for detection of antibodies against Salmonella, employing a stable coating of lipopolysaccharide derived antigen covalently attached to polystyrene microwells. Journal of Veterinary Diagnosis and Investigations, 12: 130-135. 
    5. Chandra M, Singh R, Shankar H, Agarwal M, Agarwal RK, Sharma G and Babu N (2007). Seroprevalence of Salmonella among goats slaughtered for chevon in Bareilly (Northern India). Preventive Veterinary Medicine, 80: 1-8.
    6. Munford RS, Hall CL and Rick PD (1980). Size heterogeneity of Salmonella Typhimurium lipopolysaccharides in outer-membranes and culture supernatant membrane fragments. Journal of Bacteriology, 14: 630-640.
    7. Rezania S, Amirmozaffari N, Tabarraei B, Jeddi-Tehrani M, Zarei O, Alizadeh R, Masjedian F and Zarnani AH (2011). Extraction, purification and characterization of lipopolysaccharide from Escherichia coli and Salmonella typhi . Avicenna Journal of Medical Biotechnology, 3(1):3-9.
    8. Sachan N, Nautiyal B, Agarwal RK, Singh VP and Verma AK (2013). Sero-epidemiological pattern of salmonellosis in animals of Rohilkhand region, Uttar Pradesh, India Journal of Animal Health and Production, 1 (1): 6–9.
    9. Saxena SN, Kumari N, Saini SS, Soni DV, Pahwa RK, Jayasheela M (1989). Surveillance of Salmonella in India for drug Resistance. Indian Journal of Medical Science, 43: 145-150.
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