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Research Article

volume 52 issue 8 (august 2018) : 1124-1128,   Doi: 10.18805/ijar.B-3342

Single Nucleotide Polymorphism in KiSS1 gene and its association with semen quality in Bos taurus and Bos indicus bulls

P
P. Divya
K
K.P. Ramesha
R
Ragini Kumari
A
Arun Pratap Singh
D
D.N. Das
M
M. Basavaraju
U
U.T. Mundhe
1ICAR- NDRI Southern Regional Station, Adugodi, Bengaluru-560 030, Karnataka, India
Cite article:- Divya P., Ramesha K.P., Kumari Ragini, Singh Pratap Arun, Das D.N., Basavaraju M., Mundhe U.T. (2018). Single Nucleotide Polymorphism in KiSS1 gene and its association with semen quality in Bos taurus and Bos indicus bulls. Indian Journal of Animal Research. 52(8): 1124-1128. doi: 10.18805/ijar.B-3342.

ABSTRACT

Selection of high fertile bulls with the help of marker assisted selection has gained importance in recent years. The low heritability of fertility traits hampers improvement of these traits by conventional selection based on phenotypic records. No information is available on the role of SNPs in KiSS1 gene in cattle on semen quality parameters in bovines. KiSS1 genes code for Kisspeptin, which are essential upstream regulators of neurons secreting gonadotropin-releasing hormone and play crucial role in reproduction.The coding regions along with exon-intron boundaries of KiSS1 gene, was characterized using PCR-SSCP method and direct sequencing. Two genotypes were observed which were represented as SSCP pattern 1 and pattern 2 and found to carry one  SNPs (T153C) and one insertion of G at 291_292bp. The bulls with pattern 2 were heterozygous with respect to the transition T153C and pattern1 bulls were homozygous with TT genotype. The transition was predicted to cause amino acid change from Valine to Alanine. The frequency of bulls with pattern1 and pattern 2 were 0.67 and 0.33 in 67 Holstein Friesian bulls and 0.73 and 0.27 in 13 Khillari bulls. The association study of genotypes with semen quality parameters revealed significant association of genotypes with acrosome integrity in fresh semen (P<0.05) and no association with sperm concentration, volume per ejaculate, percent live sperm and Hypo Osmotic Swelling Test (HOST) with higher acrosome integrity in bulls with pattern2. Upon validation of the results in larger population and identifying the exact role of the novel SNP T153C and insertion of G at 291_292bp, they could be incorporated in selection programme for improving fertility in bulls.as markers for acrosome integrity in cattle.

REFERENCES

  1. Ahmed, E.A., Hayato, S., Tatsuru, S., Tomoyoshi, Y., Tetsuro, Y., Toh-Ichi H., Ken, S. Tsutomu, H. (2009). Characteristics of the Stimulatory Effect of Kisspeptin-10 on the Secretion of Luteinizing Hormone, Follicle-Stimulating Hormone and Growth Hormone in Prepubertal Male and Female Cattle. Journal of Reproduction and Development, 55(6): 650-654.
  2. An, X., P, Han, P., Hou J., X., Zhao, H. B., Yan, Y., Ma, T., Fang, F., Meng, F.X., Song, Y.X., Wang, J.G. Cao, B.Y. (2010). Molecular cloning and characterization of KiSS1 promoter and effect of KiSS1 gene mutations on litter size in the goat. Genetics and Molecular Research, 12(4): 4308-4316.
  3. An, X.P., Ma, T., Hou, J.X., Fang, F., Han, P., Yan, Y., Zhao, H., Song, Y., Wang, J., Cao, B. (2013). Association analysis between variants in KiSS1 gene and litter size in goats. BMC Genetics,14: 63-68.
  4. Blom, E. (1950). A rapid staining method using eosin-nigrosin to distinguish between live and dead spermatozoa. Animal Breeding Abstracts,18: 1390. 
  5. Caraty, A., Franceschini, I.,Hoffman G.E. (2010). Kisspeptin and the preovulatory gonadotrophin-releasing hormone/ luteinising hormone surge in the ewe: basic aspects and potential applications in the control of ovulation. Journal of Neuroendocrinology,22: 710-715.
  6. Correa, J.R. and Zavos, P.M. (1994). The hypo-osmotic swelling test; its employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane. Theriogenology,42(2): 351-360. 
  7. Dhillo, W.S., Chaudhri, O.B., Patterson, M., Thompson, E.L., Murphy, K.G., Badman, M.K., McGowan, B.M., Amber, V., Patel S., Ghatei, M.A., Bloom, S.R. (2005). Kisspeptin-54 stimulates the hypothalamic-pituitary gonadal axis in human males. The Journal of Clinical Endocrinology & Metabolism,90: 6609–6615. 
  8. Gottsch, M.L., Cunningham, M.J., Smith, J.T., Popa, S.M., Acohido, B.V., Crowley, W.F., Seminara, S., Clifton, D.K. Steiner, R.A. (2004). A role of KiSSpeptins in the regulation of gonadotropin secretion in the mouse. Endocrinology,145: 4073–4077.
  9. Hancock, J.L. (1952). The morphology of bull spermatozoa. Journal of Experimental Biology,29(3): 445-453.
  10. Irwig, M.S., Fraley, G.S., Smith, J.T., Acohido, B.V., Popa, S.M., Cunningham, M.J., Gottsch, M.L., Clifton, D.K., Steiner, R.A. (2004). KiSSpeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS- 1 mRNA in the male rat. Neuroendocrinology,80: 264-272.
  11. Miller, S.A., Dykes, D., Polesky, H.F. (1988). A sample salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research,16(3): 1215. 
  12. Navarro, V.M., Castellano, J.M., Fernández-Fernández, R., Barreiro, M.L., Roa, J., Sanchez-Criado, J.E., Aguilar, E., Dieguez, C., Pinilla, L., Tena-Sempere, M. (2004). Developmental and hormonally regulated messenger ribonucleic acid expression of KiSS-1 and its putative receptor, GPR54, in rat hypothalamus and potent luteinizing hormone-releasing activity of KiSS-1 peptide. Endocrinology,145: 4565-4574.
  13. Okamura, H., Yamamura, T., Wakabayashi, Y. (2013). Kisspeptin as a master player in the central control of reproduction in mammals: an overview of Kisspeptin research in domestic animals. Animal Science Journal,84(5): 369-381.
  14. Patterson, M., Murphy, K.G., Thompson, E.L., Patel, S., Ghatei, M.A., Bloom, S.R. (2006). Administration of Kisspeptin-54 into discrete regions of the hypothalamus potently increases plasma luteinising hormone and testosterone in male adult rats. Journal of Neuroendocrinology,18: 349-354.
  15. Ramesha, K.P., Khillar breed of cattle. Seminar on “Indigenous cattle and their role in the new millennium”, 24-25th March, 2001 at Erode, Tamilnadu, India.
  16. Sambrook, J. and Russell, D.W. Molecular Cloning: A Laboratory Manual 3rd edition. Cold Spring Harbour, Cold Spring Laboratory Press, 2001; NY.
  17. Singh, A.P., Ramesha, K.P., Isloor, S., Divya, P., Akhila Rao., Basavaraju, M., Das, D.N., Munde, U. (2015). Single nucleotide polymorphisms in lactoferrin gene are associated with lactoferrin content in milk and somatic cell count in Deoni (Bos indicus) cows. Pakistan Veterinary Journal,35(3): 303-308.
  18. Smith, J.T., Dungan, H.M., Stoll, E.A., Gottsch, M.L., Braun, R.E., Eacker, S.M., Clifton, D.K. Steiner, R.A. (2005). Differential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouse. Endocrinology,146: 2976–2984.
  19. Sonika, A., Rekha, S., Maitra, A., Borana, K., Tantia, M.S. Ved Prakash. (2015). Association analysis of a novel SNP in GPR54 gene with reproductive traits in Indian goats. Indian Journal of Dairy Science,68(1): 39-44.
  20. Tovar, S., Vázquez, M.J., Navarro, V.M., Fernandez-Fernandez, R., Castellano, J.M., Vigo, E., Roa, J., Casanueva, F.F., Aguilar, E., Pinilla, L., Dieguez, C., Tena-Sempere, M. (2006). Effects of single or repeated intravenous administration of Kisspeptin upon dynamic LH secretion in conscious male rats. Endocrinology,147: 2696-2704. 
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 8 (august 2018) : 1124-1128,   Doi: 10.18805/ijar.B-3342

    Single Nucleotide Polymorphism in KiSS1 gene and its association with semen quality in Bos taurus and Bos indicus bulls

    P
    P. Divya
    K
    K.P. Ramesha
    R
    Ragini Kumari
    A
    Arun Pratap Singh
    D
    D.N. Das
    M
    M. Basavaraju
    U
    U.T. Mundhe
    1ICAR- NDRI Southern Regional Station, Adugodi, Bengaluru-560 030, Karnataka, India
    Cite article:- Divya P., Ramesha K.P., Kumari Ragini, Singh Pratap Arun, Das D.N., Basavaraju M., Mundhe U.T. (2018). Single Nucleotide Polymorphism in KiSS1 gene and its association with semen quality in Bos taurus and Bos indicus bulls. Indian Journal of Animal Research. 52(8): 1124-1128. doi: 10.18805/ijar.B-3342.

    ABSTRACT

    Selection of high fertile bulls with the help of marker assisted selection has gained importance in recent years. The low heritability of fertility traits hampers improvement of these traits by conventional selection based on phenotypic records. No information is available on the role of SNPs in KiSS1 gene in cattle on semen quality parameters in bovines. KiSS1 genes code for Kisspeptin, which are essential upstream regulators of neurons secreting gonadotropin-releasing hormone and play crucial role in reproduction.The coding regions along with exon-intron boundaries of KiSS1 gene, was characterized using PCR-SSCP method and direct sequencing. Two genotypes were observed which were represented as SSCP pattern 1 and pattern 2 and found to carry one  SNPs (T153C) and one insertion of G at 291_292bp. The bulls with pattern 2 were heterozygous with respect to the transition T153C and pattern1 bulls were homozygous with TT genotype. The transition was predicted to cause amino acid change from Valine to Alanine. The frequency of bulls with pattern1 and pattern 2 were 0.67 and 0.33 in 67 Holstein Friesian bulls and 0.73 and 0.27 in 13 Khillari bulls. The association study of genotypes with semen quality parameters revealed significant association of genotypes with acrosome integrity in fresh semen (P<0.05) and no association with sperm concentration, volume per ejaculate, percent live sperm and Hypo Osmotic Swelling Test (HOST) with higher acrosome integrity in bulls with pattern2. Upon validation of the results in larger population and identifying the exact role of the novel SNP T153C and insertion of G at 291_292bp, they could be incorporated in selection programme for improving fertility in bulls.as markers for acrosome integrity in cattle.

    REFERENCES

    1. Ahmed, E.A., Hayato, S., Tatsuru, S., Tomoyoshi, Y., Tetsuro, Y., Toh-Ichi H., Ken, S. Tsutomu, H. (2009). Characteristics of the Stimulatory Effect of Kisspeptin-10 on the Secretion of Luteinizing Hormone, Follicle-Stimulating Hormone and Growth Hormone in Prepubertal Male and Female Cattle. Journal of Reproduction and Development, 55(6): 650-654.
    2. An, X., P, Han, P., Hou J., X., Zhao, H. B., Yan, Y., Ma, T., Fang, F., Meng, F.X., Song, Y.X., Wang, J.G. Cao, B.Y. (2010). Molecular cloning and characterization of KiSS1 promoter and effect of KiSS1 gene mutations on litter size in the goat. Genetics and Molecular Research, 12(4): 4308-4316.
    3. An, X.P., Ma, T., Hou, J.X., Fang, F., Han, P., Yan, Y., Zhao, H., Song, Y., Wang, J., Cao, B. (2013). Association analysis between variants in KiSS1 gene and litter size in goats. BMC Genetics,14: 63-68.
    4. Blom, E. (1950). A rapid staining method using eosin-nigrosin to distinguish between live and dead spermatozoa. Animal Breeding Abstracts,18: 1390. 
    5. Caraty, A., Franceschini, I.,Hoffman G.E. (2010). Kisspeptin and the preovulatory gonadotrophin-releasing hormone/ luteinising hormone surge in the ewe: basic aspects and potential applications in the control of ovulation. Journal of Neuroendocrinology,22: 710-715.
    6. Correa, J.R. and Zavos, P.M. (1994). The hypo-osmotic swelling test; its employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane. Theriogenology,42(2): 351-360. 
    7. Dhillo, W.S., Chaudhri, O.B., Patterson, M., Thompson, E.L., Murphy, K.G., Badman, M.K., McGowan, B.M., Amber, V., Patel S., Ghatei, M.A., Bloom, S.R. (2005). Kisspeptin-54 stimulates the hypothalamic-pituitary gonadal axis in human males. The Journal of Clinical Endocrinology & Metabolism,90: 6609–6615. 
    8. Gottsch, M.L., Cunningham, M.J., Smith, J.T., Popa, S.M., Acohido, B.V., Crowley, W.F., Seminara, S., Clifton, D.K. Steiner, R.A. (2004). A role of KiSSpeptins in the regulation of gonadotropin secretion in the mouse. Endocrinology,145: 4073–4077.
    9. Hancock, J.L. (1952). The morphology of bull spermatozoa. Journal of Experimental Biology,29(3): 445-453.
    10. Irwig, M.S., Fraley, G.S., Smith, J.T., Acohido, B.V., Popa, S.M., Cunningham, M.J., Gottsch, M.L., Clifton, D.K., Steiner, R.A. (2004). KiSSpeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS- 1 mRNA in the male rat. Neuroendocrinology,80: 264-272.
    11. Miller, S.A., Dykes, D., Polesky, H.F. (1988). A sample salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research,16(3): 1215. 
    12. Navarro, V.M., Castellano, J.M., Fernández-Fernández, R., Barreiro, M.L., Roa, J., Sanchez-Criado, J.E., Aguilar, E., Dieguez, C., Pinilla, L., Tena-Sempere, M. (2004). Developmental and hormonally regulated messenger ribonucleic acid expression of KiSS-1 and its putative receptor, GPR54, in rat hypothalamus and potent luteinizing hormone-releasing activity of KiSS-1 peptide. Endocrinology,145: 4565-4574.
    13. Okamura, H., Yamamura, T., Wakabayashi, Y. (2013). Kisspeptin as a master player in the central control of reproduction in mammals: an overview of Kisspeptin research in domestic animals. Animal Science Journal,84(5): 369-381.
    14. Patterson, M., Murphy, K.G., Thompson, E.L., Patel, S., Ghatei, M.A., Bloom, S.R. (2006). Administration of Kisspeptin-54 into discrete regions of the hypothalamus potently increases plasma luteinising hormone and testosterone in male adult rats. Journal of Neuroendocrinology,18: 349-354.
    15. Ramesha, K.P., Khillar breed of cattle. Seminar on “Indigenous cattle and their role in the new millennium”, 24-25th March, 2001 at Erode, Tamilnadu, India.
    16. Sambrook, J. and Russell, D.W. Molecular Cloning: A Laboratory Manual 3rd edition. Cold Spring Harbour, Cold Spring Laboratory Press, 2001; NY.
    17. Singh, A.P., Ramesha, K.P., Isloor, S., Divya, P., Akhila Rao., Basavaraju, M., Das, D.N., Munde, U. (2015). Single nucleotide polymorphisms in lactoferrin gene are associated with lactoferrin content in milk and somatic cell count in Deoni (Bos indicus) cows. Pakistan Veterinary Journal,35(3): 303-308.
    18. Smith, J.T., Dungan, H.M., Stoll, E.A., Gottsch, M.L., Braun, R.E., Eacker, S.M., Clifton, D.K. Steiner, R.A. (2005). Differential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouse. Endocrinology,146: 2976–2984.
    19. Sonika, A., Rekha, S., Maitra, A., Borana, K., Tantia, M.S. Ved Prakash. (2015). Association analysis of a novel SNP in GPR54 gene with reproductive traits in Indian goats. Indian Journal of Dairy Science,68(1): 39-44.
    20. Tovar, S., Vázquez, M.J., Navarro, V.M., Fernandez-Fernandez, R., Castellano, J.M., Vigo, E., Roa, J., Casanueva, F.F., Aguilar, E., Pinilla, L., Dieguez, C., Tena-Sempere, M. (2006). Effects of single or repeated intravenous administration of Kisspeptin upon dynamic LH secretion in conscious male rats. Endocrinology,147: 2696-2704. 
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