Evaluation and comparison of immune responsiveness to sheep red blood cells, PHA-P and IBDV vaccine in divergent stocks of chicken

DOI: 10.18805/ijar.v0iOF.8457    | Article Id: B-3341 | Page : 1218-1222
Citation :- Evaluation and comparison of immune responsiveness to sheep red blood cells, PHA-P and IBDV vaccine in divergent stocks of chicken .Indian Journal Of Animal Research.2018.(52):1218-1222

Prajwalita Pathak, P. P. Dubey, S. K. Dash, D. Deka and Varinder Raina

prajwalitapathak@gmail.com
Address :

Department of Animal Genetics and Breeding, Directorate of Livestock Farms, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.

Submitted Date : 24-11-2016
Accepted Date : 17-02-2017

Abstract

A total of 90 birds comprising two native breeds viz. Aseel and Kadaknath and one synthetic broiler stock i.e. IBL-80 were utilized to evaluate and compare antibody response to Sheep Red Blood Cells by haemagglutination test at 0, 5 and 10 days post primary inoculation, to study in vivo cell mediated immune response to mitogen Phytohaemagglutinin (PHA-P) and to evaluate immune responsiveness to IBDV vaccine. The presence of natural antibodies against SRBC was evident in all the genetic groups. All groups showed an increase in HA titre upto 10 days post immunization. The HA titre on 10 day was significantly higher in Aseel (1.88±0.10) followed by IBL-80 (1.13±0.05) and Kadaknath (1.09±0.06). However, the differences among Kadaknath and IBL-80 at day 10 PPI were failed to attain statistical significance. The in vivo cell mediated response to mitogen was highest in Aseel (0.68 mm) followed by IBL-80 (0.59 mm) and Kadaknath (0.43 mm).There was significant difference between the layer breeds for response to phytoheamagglutinin but IBL-80 was not significantly differ from both Aseel and Kadaknath. The titre values for IBDV were lowest before immunization and got increased during 7, 14, 21 DPI.  At 14 DPI the titre value were significantly different in all the breeds in which Aseel exhibited the highest titre value (2.96±0.04) followed by IBL-80 (2.77±0.09) and Kadaknath (2.64±0.06). It was found that at 21 DPI antibody response was highest in all the breeds, however differences in titre value at 21 DPI in different breeds were not significantly different.
 

Keywords

Aseel Immune responsiveness Kadaknath Phytohaemagglutinin Sheep Red Blood Cell.

References

  1. Aricibasi, M., Jung, A., Heller, E. D. and Rautenschlein, S. (2010). Differences in genetic background influence the induction of innate and acquired immune responses in chickens depending on the virulence of the infecting infectious bursal disease virus (IBDV) strain. Veterinary Immunology and Immunopathology 135 (1–2): 79-92
  2. Ashraf, M., Mahmood, S. and Ahmed, F. (2003). Comparative reproductive efficiency and egg quality characteristics of Lyallpur Silver Black and Rhode Island Red breeds of poultry. International Journal of Agriculture and Biology 5 (4): 449-451
  3. Benda, V., Hampl, A. and Trtkova, K. (1990). Evaluation of wattle reaction and antibody response in different chicken breeds.Acta Veterinaria Brunensis 59 (3-4): 151-156 (Abstr.)
  4. Cheema, M. A., Quereshi, M. A. and Havenstein, G. B. (2003). A comparison of the immune profile of commercial broiler strains when raised on marginal and high protein diets. International Journal of Poultry Science 2 (5): 300-312
  5. Cheng, S. and Lamont, S. J. (1988). Breeding and Genetics: genetic analysis of immunocompetence in White Leghorn chicken line. Poultry Science 67: 989-995
  6. Gavora, J. S. and Spencer, J. L. (1979). Studies of genetic resistance of chickens to Marek’s disease - A review. Comparative Immunology, Microbiology and Infectious Diseases 2: 359
  7. Gavora, J.S., Spencer, J. L., Okada, I. and Grunder, A. A. (1990). Correlation of genetic resistance of chicken to Marek’s disease virus with vaccination protection and in vivo response to phytohaemagglutinin. Genetics Selection Evolution 22: 457
  8. Hans, P. F. S. and Smyth, J. R. (1972). The influence of growth rate on development of Marek’s disease in chickens. Poultry Science 51: 975-985
  9. Haunshi, S. and Sharma D. (2002). Immunocompetence in native and exotic chicken populations and their crosses developed for rural farming. Indian Journal of Poultry Science 37 (1): 10-15
  10. Hussain, I., Zahoor, M. A., Rasool, M. H., Mahmood, M. S., Mansoor, M. K. and Riaz, M. N. (2003). Detection of serum antibody levels against Infectious Bursal Disease (IBD) virus using Indirect Hemagglutination (IHA) test in commercial broilers. International Journal of Poultry Science 2 (6): 442-445
  11. Kundu, A., Singh, D. P., Mohapatra, S. C., Dash, B. B., Moudgal, R. P. and Bisht, G. S. (1999a). Antibody response to sheep erythrocytes in Indian native vis-à-vis imported breeds of chicken. British Poultry Science 40: 40-43
  12. Kundu, A., Singh, D. P., Mohapatra, S. C., Dash, B. B., Moudgal, R. P. and Bisht, G. S. (1999b). Immunocompetence status of Indian native vis-à-vis imported breeds of chicken: 2-Mercaptoethanol resistance (IgG) and sensitive (1gM) antibody response to sheep erythrocytes. Indian Journal of Poultry Science 34 (3): 295-302
  13. Martin, A., Gross, W. B. and Siegel, P. B. (1989). lgG and 1gM responses in high and low antibody selected lines of chickens. Journal of Heredity 80 (3): 249-252
  14. Miller, L. L., Siegel, P. B. and Dunnington, E. A. (1992). Inheritance of antibody response to sheep erythrocytes in lines of chicken divergently selected for fifty six day body weight and their crosses. Poultry Science 71: 47-52
  15. Msoffe, P. L. M., Minga, U. M., Olsen, J. B., Yongolo, M. G. S., Juul-Madsen, H. R., Gwakisa, P. S. and Mtambo, M. M. A. (2001). Phenotypes including immunocompetence in Scavenging local chicken ecotypes in Tanzania. Tropical Animal Health and Production 33 (4): 341-354
  16. Paramentier, H. K., Vries-Reilingh, G. D. and Niewland, M. G. B. (1998). Kinetic and immunohistochemical characteristics of mitogen induced cutaneous hypersensitivity in chickens selected for antibody responsiveness. Veterinary immunology and Immunopathology 66 (3-4): 367-376 (Abstr).
  17. Parmentier, H. K., Nieuwland, M. G. B., Rijke, E., DeVries, R. G. and Schrama, J. W. (1996). Divergent antibody responses to vaccines and divergent body weights of chicken lines selected for high and low humoral responsiveness to sheep red blood cells. Avian Disease 40: 634-644
  18. Quereshi, M. A. and Havenstein, G. B. (1994). A comparison of immune performance of a commercial broiler with a random bred strain when fed typical and broiler diet. Poultry Science 73: 1805-1812
  19. Rao, S. V., Prahraj, N. K., Reddy, M. R. and Sridevi, B. (1999). Immunocompetence resistance to Escherichia coli and growth in male broiler parent chicks fed different levels of crude protein. Veterinary Research Communication 23: 323-326
  20. Reddy, B. L. N., Panda, A. K., Reddy, M. R., Rao, S. V. R. and Praharaj, N. K. (2005). Studies on the influence of juvenile growth traits on laying performance in egg type chicken. Indian Journal of Poultry Science 36 (3): 290-293
  21. Saravanan, P., Kumar, S. and Kataria, J. M. (2004). Use of multiple antigenic peptides related to antigenic determinants of infectious bursal disease virus (IBDV) for detection of anti-IBDV-specific antibody in ELISA—quantitative comparison with native antigen for their use in serodiagnosis. Journal of Immunological Methods 293 (1–2): 61–70
  22. Sivaraman, G. K. and Kumar, S. (2006). Immune responsiveness to IBDV vaccine in SDL broiler chicken lines divergently selected for immune competence index. Indian Journal of Poultry Science 41(1): 21-25 
  23. Ubosi, C. O., Gross, W. B. and Siegel, P. B. (1985). Divergent selection of chickens for antibody production to sheep erythrocytes: Age effect in parental lines and their crosses. Avian Disease 29: 150-158.
  24. Vanderzipp, A. J. (1983). Breeding for immune responsiveness and disease resistance. World’s Poultry Science Journal 62: 205-211
  25. Vanderzipp, A. J. and Leenstra, F. R. (1980). Genetic analysis of the humoral immune response of White Leghorn chickens.Poultry Science 59: 1363-1369
  26. Yunis, R., Ben-David, A., Heller, F. D. and Cahaner, A. (2000). Immunocompetence and viability under commercial conditions of broiler group differing in growth rate and in antibody response to Escherichia coli vaccine. Poultry Science 79: 810-816
     

Global Footprints