Protective effect of Psidium guajava leaf ethanolic extract against streptozotocin-induced diabetes and lipidosis in rats

DOI: 10.18805/ijar.B-3337    | Article Id: B-3337 | Page : 1190-1194
Citation :- Protective effect of Psidium guajava leaf ethanolic extract against streptozotocin-induced diabetes and lipidosis in rats.Indian Journal Of Animal Research.2018.(52):1190-1194
R. Manikandan, A.Vijaya Anand, P. Sampathkumar and N. Manoharan
Address : Department of Biochemistry, M.I.E.T Arts and Science College, Trichy- 620 007, Tamil Nadu, India.
Submitted Date : 14-11-2016
Accepted Date : 12-05-2017


This study was conducted to find out the anti-diabetic and hypolipidemic potential of ethanolic extract of Psidium guajava Linn leaves and its one of the important compound of caryophyllene in a streptozotocin (STZ) induced diabetic rats. The rats were divided into eight groups. Diabetes was induced by STZ at a dosage of 60 mg/kg b.w. The various dosages of extract (100, 200, 300 mg/kg b.w), caryophyllene (300 mg/kg b.w) were injected and glibenclamide (3 mg/kg b.w) is used as a standard drug. After the treatment of the extract and caryophyllene the levels of blood glucose, HbA1c, glucose-6-phosphatase, fructose-1,6-bis phosphatase, total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), high density lipoprotein (HDL) were determined. Pancreatic tissue changes were noticed in all the groups. The present study showed that there was a significant reduction in blood glucose, HbA1c, glucose-6-phosphatase, fructose-1,6-bis phosphatase, TC, TG, LDL, VLDL and the enhancement was noticed in the level of  insulin, glucokinase and  HDL. Pancreas was damaged in diabetic induced rats and it altered to normal size and shape in the plant extract treated and caryophyllene treated groups. The results proved that the STZ induced diabetic rat models had revealed anti-diabetic and hypolipidemic effect treated with ethanolic leaf extract of P. guajava and caryophyllene.


Antihyperglcemic Caryophyllene Hypolipidemic Psidium guajava Streptozotocin.


  1. Ahamad, F.B. and Holdsworth, D.K. (2003). Medicinal plants of Sabah, East Malaysia Part. I.Pharm Biol.4: 340-6.
  2. Arati, G. and Sachdanandam, S. (2003). Terupeutic effect of Semecarpus and Cardium Linn, nut milk extract on Carbohydrate metabolizing and mitochondrial TCA cycle and respiratory chain enzymes in mammar carcinoma rats. J.Phar and Pharmacol. 55: 1283-1290. 
  3. Arvind, K., Pradeepa, R., Deepa, R. and Mohan, V. (2002). Diabetes and coronary artery diseases, Indian J Med Res.116:163-176.
  4. Bahi Amine., Al MansouriShamma., Al MemariElyazia., Al AmeriMouza., Nurulain Syed, M. and Ojha Shreesh. (2014). b-Caryophyllene, a CB2 receptor agonist produces multiple behavioral changes relevant to anxiety and depression in mice. Physiology & Behavior.135: 119–124.
  5. Betz, J.M., Gay, M.L., Mossoba, M.M., Adams, S. and Portz, B.S. (1997). Chiral gas chromatographic determination of ephedrine-type alkaloids in dietary supplements containing Má Huáng. J AOAC Int. 80(2): 303-315.
  6. Bhavapriya, V. and Govindrajsamy, S. (2000). Biochemical on the Hypoglycemia effect Aefelmarmelos (Linn) correa Ex. Rox B. In streptozotocin induced diabetic rats. Indian Drug. 7(10): 474-477.
  7. Bopanna, K.N.,Kannan,J.,Sushmagangil, Blaraman, R. and Rathod, S.P. (1997). Anti-diabetic and anti hyperlipedemic effect of Neem, Lipedemic effect of Neem seed kernel powder on alloxon diabetic rabbits. Ind. J. Pharmacology. 29: 162-167.
  8. Cheng, J.T. and Yang, R.S. (1983). Hypoglycemic effect of guava juice in mice and human subjects. Am J Chin Med. 11: 74-6.
  9. Danaei, G., Finucane, M.M., Lu, Y., Singh, G.M., Cowan, M.J. and. Paciorek, C.J. (2011). National, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2.7 million participants. Lancet. 378(9785): 31–40. 
  10. El-Hilaly, J., Tahraoui, A., Israili, Z.H. andLyoussi, B. (2006). Hypolipidemic effects of acute and sub-chronic administration of an aqueous extract of Ajugaiva L. whole plant in normal and diabetic rats. J. Ethnopharmacol.105(3): 441-448.
  11. Gertsch, J., Leonti, M. Raduner, S. Racz, I., Chen, J.Z.. andXie, X.Q. (2008). Beta- caryophyllene is a dietary cannabinoid.Proc. Natl. Acad. Sci. U.S.A.105. 9099–9104.
  12. Goyal, R.K., Bhullar, S.S. and Singh, R. (1999). Comparative sensitivity to inorganic phosphate of sucrose to starch conversion in sorghum and wheat grains raised through culture. Plant PhysioBiochem. 28: 755.
  13. Guimaraes Santos. and Adriano. (2012). Copaiba Oil-Resin Treatment Is Neuroprotective and Reduces Neutrophil Recruitment and Microglia Activation after Motor Cortex Excitotoxic Injury. Evidence-Based Complementary and Alternative Medicine.1–9.
  14. Gutierrez, R.M.P., Mitchell, S. and Solis, R.V. (2008). Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology. 117: 1–27.
  15. Harris, R.A. and Crabb, D.W. (1982). Metabolic interrelationships. In: Textbook of Biochemistry with Clinical Correlations, Ed. Delvin TM, New York: John Wiley and Sons Inc. pp: 531-559.
  16. Hayashida, C.Y., Suzuki, K., Fujika, H., Koizumi, M., Toyota, T. and Goto, Y. (1983). Morph metrical quantization of pancreatic endocrine cells in patients with carcinoma of the pancreas. Tohoku. J. Exp.Med. 141: 311-322.
  17. Heinrich, M., Ankli, A. B., Frei, C., Weimann. And Sticher, O. (1998). Medicinal plants in Mexico healers consenscy and cultural importance. Social Science and Medicine. 47: 1859-1871.
  18. Hernandez, D.F. (1971). Plants of the Philippines. M&L Licudine Enterprises. First printing.Philippines University of the Philippines, Chairman, Consuelo. Pp:678-680.
  19. Holetz, F.B., Pessini, G.L., Sanches, N.R. ,Cortenz, D.A., Nakamura, C.V. and Filho, B.P. (2002). Screening of some plants used in the Brazilian folk medicine for the treatment of infections disease. Memoriasdo Institute Oswaldo Cruz. 97: 1027-1031.
  20. Ivorra, M.D., Paya, M. and Villar, A. (1989). A review of natural products and plants as potential antidiabetic drugs. J    Ethnopharmacol.27:243–275.
  21. Joy, P.P., Thomas, J., Samuel Mathew. and Baby, P. (1998). Skaria Medicinal plants.
  22. Karawya, M.S. and Wahab, S.A. (1984). Diphenylamine, an antihyperglycemic agent from onion and tea. J.Nat. Product. 47: 775-780.
  23. Khanna, P., Jain, S.C.,Panagariya, A. and Dixit, V.P. (1981). Hypoglycemic activity of polypeptide-p from a plant source. J.Nat. product. 44: 648-655.
  24. Lozoya, X., Meckes,M.,Abou–Zaid,M.,Tortoriello,J.,Nozzolillo, C. and Amason, J.T. (1994). Quercetin glycosides in Psidium guajava Linn leaves and determination of spasmolytic principle.Archives Medical Research. 25: 11–15.
  25. Manikandan, R., VijayaAnand, A. and Durai Muthumani, G. (2013). Phytochemical and in vitro anti-diabetic activity of ethanolic extract of Psidium guajaval eaves. Int.J.Curr.Microbiol.App.Sci. 2(2):15-19.
  26. Morrish, N.J., Wang, S.L., Stevens, L.K., Fuller, J.H..and Keen, H. (2001). Mortality and causes of death in the WHO Multinational Study of Vascular Disease in Diabetes. Diabetologia. 44 Suppl 2:S14–S21.
  27. Schimizu, M.I., Shima, T.R. and Hasimatoy, S. (1984). Inhibition of lens aldose reductase by flavonoids. Phytochemistry. 23: 1885-1888.
  28. Oh, W.K., Lee, C.H., Lee, M.S., Bae, E.Y., Sohn,C.B.,Oh, H., Kim, B.Y. and Ahn, J.S. (2005). Antidiabetic effects of extracts from Psidiumguajava.Journal of Ethnopharmacology. 96: 411-415.
  29. Pondikis, C. (1996). Psidium guajava L Guava. In Bajaj Y. (Ed), Biotechnology in Agriculture and Forestry. Vol. 35, pp:309-319.
  30. Rajiv Gandhi, G. and Sasikumar, P. (2012). Anti-diabetic effect of Merremia emarginata Burm. F. in streptozotocin induced diabetic rats. Asian Pacific Journal of Tropical Biomedicine. 2: 281-286.
  31. Ranganathan, G., Li, C. and Kern, P.A. (2000). The translational regulation of lipoprotein lipase in diabetic rats involves the 3-untransalted region of lipoprotein lipase mRNA. J.Biol.Chem. 275:40986-40991.
  32. Roy, C.K., Kamath, J.V. and Azad, M. (2006). Hepatoprotective activity of P.guajavaL leaf Extract.Indian J Exp Biol. 44(4): 305-311. 
  33. Saad Sabbar Dahham, M., Yasser., Tabana, E., Loiy., Ahmed Hassan, B., Mohamed., KhadeerAhamed.,Aman Shah Abdul Majid. and Amin Malik Shah Abdul Majid. (2016). In vitro antimeta static activity of Agarwood (Aquilariacrassna) essential oils against pancreatic cancer cells.Alexandria Journal of Medicine.52(2): 141–150.
  34. Saravanan, R., Prasad, N.R. and Pugalandi, K.V. (2003). Effect of Piper betle leaf extract on alcoholic toxicity in the rat brain. J Med Food. 6(3): 261-265.
  35. Smith, J. and Nigel, H. Trophical forests and their crops. Cornell University. Press London,
  36. Stone, B. (1970). The ûora of Guam. Micronesica. 6: 454–455
  37. Taju, G., Jayanthi, M., Basha, A., Nazeer.,Nambi, K.S., Nathiga. andSivaraj. (2010). A Hepatoprotective Effect of Indian Medicinal plant P.guajava Linn. leaf extract on paracetamol induced liver toxicity in Albino rats. Journal of Pharmacy Research. 3(8): p1759-    1763.
  38. Tanaka, T., Ishida,N.,Ishimatsu,M. Nonaka, G. and Nishioka, I. (1992). Tannins and related compounds. CXVI. Six new complex tannins, guajavins, psidinins and psiguavin from the bark of Psidium guajava L. Chemical and Pharmaceutical Bulletin. 40: 2092–    2098.
  39. Tomada, M.,Shimada.K., Konno, C. and Hikin, H.J. (1985). Structure of Panaxan B. J. A. Hypoglycaemic glycan of panaxginesg roots. Phytochemistry. 24: 2431- 2433.
  40. Won Keun Oh., Chul Ho Lee. And Myung Sun Lee. (2005). Anti-diabetic effects of extracts from Psidium guajava. Journal of Ethnopharmacology. 96: 411–415.
  41. World Health Organisation. (1998). Medicinal plants in South pacific. WHO regional publications. Western Pacific Series. 19: pp-163.
  42. Zakaria, M. and Mohd, M.A. (1994). Traditional Malay medicinal plants. Penerbit FajarBakti Sudan Berhard. pp. 129–132.

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