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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 8 (august 2018) : 1151-1154,   Doi: 10.18805/ijar.B-3335

Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender

P
Pangdun Konyak
A
Ajoy Mandal
M
Mohan Mondal
C
C. Bhakat
S
S.K. Das
S
Saroj Rai
M
M.K. Ghosh
M
M. Karunakaran
1ICAR- National Dairy Research Institute, Eastern Regional Station, Kalyani-741 235, West Bengal, India
Cite article:- Konyak Pangdun, Mandal Ajoy, Mondal Mohan, Bhakat C., Das S.K., Rai Saroj, Ghosh M.K., Karunakaran M. (2018). Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender. Indian Journal of Animal Research. 52(8): 1151-1154. doi: 10.18805/ijar.B-3335.

ABSTRACT

This experiment was conducted with the aim to study the effect of replacing egg yolk with soybean lecithin (SL) for cryopreservation of Black Bengal buck semen. Sexually matured Black Bengal buck (n = 5) were used and the ejaculates were obtained using an artificial vagina method. The semen samples were pooled and diluted in Tris extender with 5% Glycerol containing either 15% egg yolk (control group) or SL at different concentrations (1% SL, 1.5% SL and 2% SL). The semen samples were filled in straws and cooled gradually to 5 °C. Semen straws were equilibrated for 3 hours at 5°C and were frozen in static liquid nitrogen vapor and stored in liquid nitrogen. Semen samples were evaluated after initial dilution, after completion of equilibration and after freeze thawing for in vitro sperm characters such as sperm motility, functional membrane integrity and malondioldehyde (MDA) concentration. Semen samples preserved in extender containing 1% SL was able to maintain in vitro sperm characters similar to the extender containing egg yolk. However, significant (P<0.05) reduction in all semen parameters was observed as the concentration of soybean lecithin increased above 1% level. It is concluded that an extender containing soybean lecithin @ 1% with 5% Glycerol can be used for replacing egg yolk for cryopreservation of Black Bengal buck semen. 

REFERENCES

  1. Ahmad, M., Ahmad N., Riaz A. and Anzar M. (2014). Sperm survival kinetics different types of bull semen: progressive motility, plasma membrane integrity, acrosomal status and reactive oxygen species generation. Reprod. Fertil. Dev., 27: 784-793.
  2. Akhter, S., Ansari M.S., Rakha B.A., Andrabi S.M.H., Iqbal S. and Ullah N. (2010). Cryopreservation of buffalo (Bubalus bubalis) semen in Bioxcell extender. Theriogenology, 74: 951–955.
  3. Bergeron, A. and Manjunath P. (2006). New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Mol. Reprod. Dev., 73: 1338–1344.
  4. Forouzanfar, M., Sharafi M., Hosseini S.M., Ostadhosseini S., Hajian M., Hosseini L., Abedi P., Nili N., Rahmani H.R. and Nasr-    Esfahani M.H. (2010). In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology, 73: 480–487.
  5. Futino, D., Mendes M., Matos W., Mondadori R. and Lucci C. (2010). Glycerol, methyl-formamide and dimethyl-formamide in canine semen cryopreservation. Reprod. Domest. Anim., 45: 214–220.
  6. Hammerstedt, R.H. and Amann R.P. (1993). In vitro evaluation of sperm quality: An opinion. J. Androl., 14(6): 397-406.
  7. Harvey, W.R (1975). Least squares analysis of data with unequal subclass numbers. United states Department of Agriculture, ARSH-    4, Washington, D.C.
  8. Hashida, N.H., Nor Ashikin M.N.K., Khaironizam M.Z. and Abdullah R.B. (2013). Effects of freezing on sperm velocity in four different age groups of jermasia buck. Indian J. Anim. Res., 47 (1): 54-57.
  9. Karunakaran, M. and Devanathan T. G. (2016a). Evaluation of bull semen for fertility-associated protein, in vitro characters and fertility. J. Appl. Anim. Res., 45(1): 136- 144. 
  10. Karunakaran, M., Chakurkar E. B., Ratnakaran U., Naik P. K., Mondal M., Mondal A. and Singh N. P. (2016b). Characteristics of boar semen preserved at liquid state. J. Appl. Anim. Res., 45 (1): 217–220. 
  11. Khandoker, M. A., Apu A. S., Husain S. S. and Notter D. R.(2011). A baseline survey on the availability of Black Bengal breeding bucks in different districts of Bangladesh. J. Bangladesh Agril. Univ., 9: 91–96.
  12. Moussa, M., Martinet V., Trimeche A., Tainturier D. and Anton M. (2002). Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology, 57: 1695-1706.
  13. Parmar, V.R., Suthar B.N., Nakhashi H.C., Parikh S.S. and Chauhan P.M. (2011). Study on physical characteristics of Mehsana buck semen. Indian J. Anim. Res. 45: 207 – 210.
  14. Purdy, P. (2006). A review on goat sperm cryopreservation. Small Ruminant Res. 63: 215–225.
  15. Salmani, H., Armin T., Mahdi Z., Majid B. and Mohsen S.(2014). In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology, 68:276–280.
  16. Sharafi, M., Eghbalsaied S., Nili N. and Nasr-Esfahani M. H. (2009). Ram semen in vitro fertility after cryopreservation using soybean lecithin and egg yolk based extenders. Reprod. Domest. Anim., 44: 90-95.
  17. Suleiman, S.A., Ali M.E., Zaki M.S., Malik E.M.E.A. and Nast M.A. (1996). Lipid peroxidation and human sperm motility protective role of vitamin E. J. Androl., 17(5): 530–537.
  18. Van Wagtendonk-de Leeuw, A.M., Haring R.M., Kaal-Lansbergen L.M.T.E. and Den Daas J.H.G. (2000). Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soybean extract. Theriogenology, 54:57–67. 
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    Research Article

    volume 52 issue 8 (august 2018) : 1151-1154,   Doi: 10.18805/ijar.B-3335

    Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender

    P
    Pangdun Konyak
    A
    Ajoy Mandal
    M
    Mohan Mondal
    C
    C. Bhakat
    S
    S.K. Das
    S
    Saroj Rai
    M
    M.K. Ghosh
    M
    M. Karunakaran
    1ICAR- National Dairy Research Institute, Eastern Regional Station, Kalyani-741 235, West Bengal, India
    Cite article:- Konyak Pangdun, Mandal Ajoy, Mondal Mohan, Bhakat C., Das S.K., Rai Saroj, Ghosh M.K., Karunakaran M. (2018). Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender. Indian Journal of Animal Research. 52(8): 1151-1154. doi: 10.18805/ijar.B-3335.

    ABSTRACT

    This experiment was conducted with the aim to study the effect of replacing egg yolk with soybean lecithin (SL) for cryopreservation of Black Bengal buck semen. Sexually matured Black Bengal buck (n = 5) were used and the ejaculates were obtained using an artificial vagina method. The semen samples were pooled and diluted in Tris extender with 5% Glycerol containing either 15% egg yolk (control group) or SL at different concentrations (1% SL, 1.5% SL and 2% SL). The semen samples were filled in straws and cooled gradually to 5 °C. Semen straws were equilibrated for 3 hours at 5°C and were frozen in static liquid nitrogen vapor and stored in liquid nitrogen. Semen samples were evaluated after initial dilution, after completion of equilibration and after freeze thawing for in vitro sperm characters such as sperm motility, functional membrane integrity and malondioldehyde (MDA) concentration. Semen samples preserved in extender containing 1% SL was able to maintain in vitro sperm characters similar to the extender containing egg yolk. However, significant (P<0.05) reduction in all semen parameters was observed as the concentration of soybean lecithin increased above 1% level. It is concluded that an extender containing soybean lecithin @ 1% with 5% Glycerol can be used for replacing egg yolk for cryopreservation of Black Bengal buck semen. 

    REFERENCES

    1. Ahmad, M., Ahmad N., Riaz A. and Anzar M. (2014). Sperm survival kinetics different types of bull semen: progressive motility, plasma membrane integrity, acrosomal status and reactive oxygen species generation. Reprod. Fertil. Dev., 27: 784-793.
    2. Akhter, S., Ansari M.S., Rakha B.A., Andrabi S.M.H., Iqbal S. and Ullah N. (2010). Cryopreservation of buffalo (Bubalus bubalis) semen in Bioxcell extender. Theriogenology, 74: 951–955.
    3. Bergeron, A. and Manjunath P. (2006). New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Mol. Reprod. Dev., 73: 1338–1344.
    4. Forouzanfar, M., Sharafi M., Hosseini S.M., Ostadhosseini S., Hajian M., Hosseini L., Abedi P., Nili N., Rahmani H.R. and Nasr-    Esfahani M.H. (2010). In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology, 73: 480–487.
    5. Futino, D., Mendes M., Matos W., Mondadori R. and Lucci C. (2010). Glycerol, methyl-formamide and dimethyl-formamide in canine semen cryopreservation. Reprod. Domest. Anim., 45: 214–220.
    6. Hammerstedt, R.H. and Amann R.P. (1993). In vitro evaluation of sperm quality: An opinion. J. Androl., 14(6): 397-406.
    7. Harvey, W.R (1975). Least squares analysis of data with unequal subclass numbers. United states Department of Agriculture, ARSH-    4, Washington, D.C.
    8. Hashida, N.H., Nor Ashikin M.N.K., Khaironizam M.Z. and Abdullah R.B. (2013). Effects of freezing on sperm velocity in four different age groups of jermasia buck. Indian J. Anim. Res., 47 (1): 54-57.
    9. Karunakaran, M. and Devanathan T. G. (2016a). Evaluation of bull semen for fertility-associated protein, in vitro characters and fertility. J. Appl. Anim. Res., 45(1): 136- 144. 
    10. Karunakaran, M., Chakurkar E. B., Ratnakaran U., Naik P. K., Mondal M., Mondal A. and Singh N. P. (2016b). Characteristics of boar semen preserved at liquid state. J. Appl. Anim. Res., 45 (1): 217–220. 
    11. Khandoker, M. A., Apu A. S., Husain S. S. and Notter D. R.(2011). A baseline survey on the availability of Black Bengal breeding bucks in different districts of Bangladesh. J. Bangladesh Agril. Univ., 9: 91–96.
    12. Moussa, M., Martinet V., Trimeche A., Tainturier D. and Anton M. (2002). Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology, 57: 1695-1706.
    13. Parmar, V.R., Suthar B.N., Nakhashi H.C., Parikh S.S. and Chauhan P.M. (2011). Study on physical characteristics of Mehsana buck semen. Indian J. Anim. Res. 45: 207 – 210.
    14. Purdy, P. (2006). A review on goat sperm cryopreservation. Small Ruminant Res. 63: 215–225.
    15. Salmani, H., Armin T., Mahdi Z., Majid B. and Mohsen S.(2014). In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology, 68:276–280.
    16. Sharafi, M., Eghbalsaied S., Nili N. and Nasr-Esfahani M. H. (2009). Ram semen in vitro fertility after cryopreservation using soybean lecithin and egg yolk based extenders. Reprod. Domest. Anim., 44: 90-95.
    17. Suleiman, S.A., Ali M.E., Zaki M.S., Malik E.M.E.A. and Nast M.A. (1996). Lipid peroxidation and human sperm motility protective role of vitamin E. J. Androl., 17(5): 530–537.
    18. Van Wagtendonk-de Leeuw, A.M., Haring R.M., Kaal-Lansbergen L.M.T.E. and Den Daas J.H.G. (2000). Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soybean extract. Theriogenology, 54:57–67. 
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