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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 4 (april 2018) : 530-532,   Doi: 10.18805/ijar.v0iOF.9130

Understanding motility dynamics of crossbred bull spermatozoa when analyzed by Computer Assisted Semen Analyzer (CASA)

S
Saroj Rai
S
S. Tyagi
M
M. Kumar
M
M. Karunakaran
M
M. Mondal
A
A. Mandal
R
R. Behera
1CAR- National Dairy Research Institute ERS-Kalyani, Kalyani- 741 235, West Bengal, India
Cite article:- Rai Saroj, Tyagi S., Kumar M., Karunakaran M., Mondal M., Mandal A., Behera R. (2017). Understanding motility dynamics of crossbred bull spermatozoa when analyzed by Computer Assisted Semen Analyzer (CASA). Indian Journal of Animal Research. 52(4): 530-532. doi: 10.18805/ijar.v0iOF.9130.

ABSTRACT

The study was conducted to understand sperm kinetics of Frieswal bull spermatozoa using Computer Assisted Semen Analyzer (CASA). Fifty bull ejaculates were collected from ten healthy bulls that were in routine semen collection. The semen samples were diluted in Tris buffer at a concentration of 25x106 spermatozoa/ ml for analysis. Rapidly moving spermatozoa represented the one with better velocity and progressiveness while spermatozoa with medium motility had low velocity with short distance travelled in spite of its ability to move in a straight line (straightness, STR >70 %). The slow moving cells had good head and flagella movement but they followed a circular path with straight line velocity (VSL mm/ sec), linearity (LIN %) and straightenss (STR %) of 10.91, 9.00 and 22.54, respectively. Results indicated that individual sperm cells tracked by CASA as rapid, medium and slow motile were highly variable (p<0.001). However, the sperm motility between bulls varied (p<0.05) only in lateral head displacement (ALH, mm) and beat cross frequency (BCF, Hz).

REFERENCES

  1. Amann, R.P., Seidel, G.E. and Mortimer, R.G. (2000). Fertilizing potential in-vitro semen from young beef bulls containing a high and low percentage of sperm with a proximal droplet. Theriogenology. 54:1499-1515.
  2. Blasco, L. (1984). Clinical test of sperm fertilizing ability. Fertility Sterility. 41: 177-192.
  3. Boryshpolets, S., Kowalski, R.K., Dietrich, G.J., Dzyuba, B. and Ciereszko, A. (2013). Different computer-assisted sperm analysis (CASA) systems highly influence sperm motility parameters. Theriogenology. 80: 758–765.
  4. Davis, R.O. and Katz, D.F. (1993). Operational standards for CASA instruments. J. Androl. 14: 385-394.
  5. Farrell, P.B., Foote, R.H., McArdle, M.M., Trouern-Trend, V.L. and Tardif, A.L. (1996). Media and dilution procedures tested to minimize handling effects on human, rabbit and bull sperm for computer assisted sperm analysis (CASA). Journal of Andrology. 17: 293-300.
  6. Hoflack, G., Rijsselaere, T., Maes, D., Dewulf, J., Opsomer, G., de Kruif, A. and Van Soom, A. (2005). Validation and Usefulness of the Sperm Quality Analyzer (SQA II-C) for Bull.
  7. Mortimer, S.T. (1997). A critical review of the physiological importance and analysis of sperm movement in mammals. Human Reproduction Update. 3: 403-439.
  8. Muino, R., Rivera, M.M., Rigau, T., Rodriguez-Gil, J.E. and Pena, A.I. (2008). Effect of different Thawing Rates on post thaw sperm viability, kinematic parameters and motile sperm subpopulations structure of bull semen. Animal Reproduction Science. 109: 50-64.
  9. Rivera, M.M., Quintero Moreno, A., Barrera, X., Palomo, M.J., Rigua, T. and Rodriguez-Gil, J.E. (2005). Natural Mediterranean photoperiod does not affect the main parameters of boar-semen quality analysis. Theriogenology. 64: 934-946.
  10. Scott, M.A. (2000). A glimpse at sperm function in vivo: sperm transport and epithelial interaction in the female reproductive tract. Animal Reproduction Science. 60: 337-348.
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 4 (april 2018) : 530-532,   Doi: 10.18805/ijar.v0iOF.9130

    Understanding motility dynamics of crossbred bull spermatozoa when analyzed by Computer Assisted Semen Analyzer (CASA)

    S
    Saroj Rai
    S
    S. Tyagi
    M
    M. Kumar
    M
    M. Karunakaran
    M
    M. Mondal
    A
    A. Mandal
    R
    R. Behera
    1CAR- National Dairy Research Institute ERS-Kalyani, Kalyani- 741 235, West Bengal, India
    Cite article:- Rai Saroj, Tyagi S., Kumar M., Karunakaran M., Mondal M., Mandal A., Behera R. (2017). Understanding motility dynamics of crossbred bull spermatozoa when analyzed by Computer Assisted Semen Analyzer (CASA). Indian Journal of Animal Research. 52(4): 530-532. doi: 10.18805/ijar.v0iOF.9130.

    ABSTRACT

    The study was conducted to understand sperm kinetics of Frieswal bull spermatozoa using Computer Assisted Semen Analyzer (CASA). Fifty bull ejaculates were collected from ten healthy bulls that were in routine semen collection. The semen samples were diluted in Tris buffer at a concentration of 25x106 spermatozoa/ ml for analysis. Rapidly moving spermatozoa represented the one with better velocity and progressiveness while spermatozoa with medium motility had low velocity with short distance travelled in spite of its ability to move in a straight line (straightness, STR >70 %). The slow moving cells had good head and flagella movement but they followed a circular path with straight line velocity (VSL mm/ sec), linearity (LIN %) and straightenss (STR %) of 10.91, 9.00 and 22.54, respectively. Results indicated that individual sperm cells tracked by CASA as rapid, medium and slow motile were highly variable (p<0.001). However, the sperm motility between bulls varied (p<0.05) only in lateral head displacement (ALH, mm) and beat cross frequency (BCF, Hz).

    REFERENCES

    1. Amann, R.P., Seidel, G.E. and Mortimer, R.G. (2000). Fertilizing potential in-vitro semen from young beef bulls containing a high and low percentage of sperm with a proximal droplet. Theriogenology. 54:1499-1515.
    2. Blasco, L. (1984). Clinical test of sperm fertilizing ability. Fertility Sterility. 41: 177-192.
    3. Boryshpolets, S., Kowalski, R.K., Dietrich, G.J., Dzyuba, B. and Ciereszko, A. (2013). Different computer-assisted sperm analysis (CASA) systems highly influence sperm motility parameters. Theriogenology. 80: 758–765.
    4. Davis, R.O. and Katz, D.F. (1993). Operational standards for CASA instruments. J. Androl. 14: 385-394.
    5. Farrell, P.B., Foote, R.H., McArdle, M.M., Trouern-Trend, V.L. and Tardif, A.L. (1996). Media and dilution procedures tested to minimize handling effects on human, rabbit and bull sperm for computer assisted sperm analysis (CASA). Journal of Andrology. 17: 293-300.
    6. Hoflack, G., Rijsselaere, T., Maes, D., Dewulf, J., Opsomer, G., de Kruif, A. and Van Soom, A. (2005). Validation and Usefulness of the Sperm Quality Analyzer (SQA II-C) for Bull.
    7. Mortimer, S.T. (1997). A critical review of the physiological importance and analysis of sperm movement in mammals. Human Reproduction Update. 3: 403-439.
    8. Muino, R., Rivera, M.M., Rigau, T., Rodriguez-Gil, J.E. and Pena, A.I. (2008). Effect of different Thawing Rates on post thaw sperm viability, kinematic parameters and motile sperm subpopulations structure of bull semen. Animal Reproduction Science. 109: 50-64.
    9. Rivera, M.M., Quintero Moreno, A., Barrera, X., Palomo, M.J., Rigua, T. and Rodriguez-Gil, J.E. (2005). Natural Mediterranean photoperiod does not affect the main parameters of boar-semen quality analysis. Theriogenology. 64: 934-946.
    10. Scott, M.A. (2000). A glimpse at sperm function in vivo: sperm transport and epithelial interaction in the female reproductive tract. Animal Reproduction Science. 60: 337-348.
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