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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 1 (january 2018) : 144-147,   Doi: 10.18805/ijar.B-3301

In vivo anti-tumor efficacy of Croton oblongifolius in DMBA induced mammary tumor in rats

G
Gowrav Adiga P.
N
N.B. Shridhar
J
Jagadeesh S. Sanganal
S
Suguna Rao
N
N. Shilpa
V
V. Aparna
I
I.M. Santhosh
M
M. Tirumala
B
B.M. Sindhu
1Department of Veterinary Pharmacology and Toxicology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Hebbal, Bengaluru-560 024, Karnataka, India.
Cite article:- P. Adiga Gowrav, Shridhar N.B., Sanganal S. Jagadeesh, Rao Suguna, Shilpa N., Aparna V., Santhosh I.M., Tirumala M., Sindhu B.M. (2017). In vivo anti-tumor efficacy of Croton oblongifolius in DMBA induced mammary tumor in rats. Indian Journal of Animal Research. 52(1): 144-147. doi: 10.18805/ijar.B-3301.

ABSTRACT

The present study was designed to evaluate anti-tumor activity of the methanolic extract of stem bark of Croton oblongifolius in Sprague Dawley rats. The tumor was induced in rats by DMBA given orally and intramammarily and challenging with plant extract. After obtaining suitable mass of tumor, the extract was gavaged to rats @ 200, 500 and 800 mg/kg which showed reduction in mammary tumor volume in dose-dependent manner, which was supported by histopathological observations of the treatment groups. 

REFERENCES

  1. Ahmed, B., Alam, T., Varshney, M. and Khan, S.A. (2002). Hepatoprotective activity of two plants belonging to the Apiaceae and the Euphorbiaceae family. J. Ethnopharmacol., 79: 313-316.
  2. Anazetti, M.C., Melo, P.S., Duran, N. and Marcela Haun, M. (2003). Comparative cytotoxicity of dimethylamidecrotonin in the promyelocytic leukemia cell line (HL60) and human peripheral blood mononuclear cells. Toxicol. 188(3): 261-274.
  3. Barros, A.C.S.D., Naomi, E.K., Muranaka., Mori, J.L., Helena, C.T., Pelizon., Iriya, K., Giocondo, G. and Pinotti, J.A., (2004). Induction of experimental mammary carcinogenesis in rats with 7, 12-dimethylbenz (a)anthracene. Rev. Hosp. Clín. Fac. Med. S. Paulo. 59 (5): 257-261. 
  4. Dhanya Venugopalan Nair., Shridhar, N.B. and Jayakumar, K. (2015). Evaluation of anticancer activity of Asteracantha longifolia in 7,12-Dimethylbenz(a)anthracene-induced mammary gland carcinogenesis in Sprague Dawley rats. Int. J. Nutr. Pharmacol. Neurol. Dis. 5(1):28-33.
  5. Greenacre, C. (2004). Spontaneous tumors of small mammals. Vet. Clin. North Am Exot. Anim. Pract . 7(3): 627-51.
  6. Luna, L.G. (1968). Manual of histologic staining methods of the Armed Forces Institute of Pathology. Edn. 3rd.,McG row Hill Book Co., New York, pp 1-46. 
  7. Munson, L. and Moresco, A. (2007). Comparative pathology of mammary gland cancers in domestic and wild animals. Breast Dis. 28: 7-21.
  8. Ngamrojnavanich, N., Sirimongkon, S., Roengsumran, S., Petsom, A., and Kamimura, H. (2003). Inhibition of Na+K+-ATPase activity by (-)-entKaur-16-en-19-oic acid and its derivatives. Planta. Med. 69(6): 555-556 .
  9. Pattanayak, S.P. and Sunita, P. (2008). Anti-tumor potency and toxicology of an Indian ayurvedic plant Hygrophila spinosa. Pharmacol online. 2(1): 361-371.
  10. Roengsumran, S., Musikul, K., Petsom, A., Vilaivan, T., Sangvanich ., Pornpakakul, P.S., Puthong. S., Chaichantipyuth, C., Jaiboon, N. and Chaichit, N. (2002). Croblongifolin, a new anticancer clerodane from Croton oblongifolius. Planta. Med. 68(3): 274-277.
  11. Samy, R.P., Gopalakrishnakone, P. and Ignacimuthu, S. (2006). Anti-tumor promoting potential of luteolin against 7,12-dimethylbenz(a)- anthracene induced mammary tumors in rats. Chem. Biol. Interact. 164: 1-14.
  12. Santhosh Kumar, C.N. (2014). In vitro and in vivo evaluation of anticancer property of Astracanthus longifolia in rats. PhD thesis, Karnataka Veterinary Animal and Fisheries Sciences University, Bidar, India.
  13. Sommit, D., Petsom, A., Ishikawa, T. and Roengsumran, S. (2003). Cytotoxic activity of natural labdanes and their semi-synthetic modified derivatives from Croton oblongifolius. Planta. Med. 69(2): 167-170.
  14. Thun, M.J., Delancey, J.O., Center, M.M., Jemal, A. and Ward, E.M. (2009). The global burden of cancer: Priorities for prevention. Carcinogenesis. 31(1): 100-110.
  15. Tomy, D.V. (2013). Evaluation of anti-oxidant and anti-tumour activities of Croton laevigatus. B.Pharma thesis, RVS College of Pharmaceutical Sciences, Coimbatore, India.
  16. Umadevi, M., Kumar, S.K.P., Bhowmik, D. and Vel, D.S. (2013). Traditionally used anticancer herbs in India. J. Med. Plants Studies. 1(3):56-74.
  17. Viste, J., Myers, S., Singh, B. and Simko, E. 2002. Feline mammary adenocarcinoma: tumor size as a prognostic indicator. Can Vet J. 43(1): 33-37.
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 1 (january 2018) : 144-147,   Doi: 10.18805/ijar.B-3301

    In vivo anti-tumor efficacy of Croton oblongifolius in DMBA induced mammary tumor in rats

    G
    Gowrav Adiga P.
    N
    N.B. Shridhar
    J
    Jagadeesh S. Sanganal
    S
    Suguna Rao
    N
    N. Shilpa
    V
    V. Aparna
    I
    I.M. Santhosh
    M
    M. Tirumala
    B
    B.M. Sindhu
    1Department of Veterinary Pharmacology and Toxicology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Hebbal, Bengaluru-560 024, Karnataka, India.
    Cite article:- P. Adiga Gowrav, Shridhar N.B., Sanganal S. Jagadeesh, Rao Suguna, Shilpa N., Aparna V., Santhosh I.M., Tirumala M., Sindhu B.M. (2017). In vivo anti-tumor efficacy of Croton oblongifolius in DMBA induced mammary tumor in rats. Indian Journal of Animal Research. 52(1): 144-147. doi: 10.18805/ijar.B-3301.

    ABSTRACT

    The present study was designed to evaluate anti-tumor activity of the methanolic extract of stem bark of Croton oblongifolius in Sprague Dawley rats. The tumor was induced in rats by DMBA given orally and intramammarily and challenging with plant extract. After obtaining suitable mass of tumor, the extract was gavaged to rats @ 200, 500 and 800 mg/kg which showed reduction in mammary tumor volume in dose-dependent manner, which was supported by histopathological observations of the treatment groups. 

    REFERENCES

    1. Ahmed, B., Alam, T., Varshney, M. and Khan, S.A. (2002). Hepatoprotective activity of two plants belonging to the Apiaceae and the Euphorbiaceae family. J. Ethnopharmacol., 79: 313-316.
    2. Anazetti, M.C., Melo, P.S., Duran, N. and Marcela Haun, M. (2003). Comparative cytotoxicity of dimethylamidecrotonin in the promyelocytic leukemia cell line (HL60) and human peripheral blood mononuclear cells. Toxicol. 188(3): 261-274.
    3. Barros, A.C.S.D., Naomi, E.K., Muranaka., Mori, J.L., Helena, C.T., Pelizon., Iriya, K., Giocondo, G. and Pinotti, J.A., (2004). Induction of experimental mammary carcinogenesis in rats with 7, 12-dimethylbenz (a)anthracene. Rev. Hosp. Clín. Fac. Med. S. Paulo. 59 (5): 257-261. 
    4. Dhanya Venugopalan Nair., Shridhar, N.B. and Jayakumar, K. (2015). Evaluation of anticancer activity of Asteracantha longifolia in 7,12-Dimethylbenz(a)anthracene-induced mammary gland carcinogenesis in Sprague Dawley rats. Int. J. Nutr. Pharmacol. Neurol. Dis. 5(1):28-33.
    5. Greenacre, C. (2004). Spontaneous tumors of small mammals. Vet. Clin. North Am Exot. Anim. Pract . 7(3): 627-51.
    6. Luna, L.G. (1968). Manual of histologic staining methods of the Armed Forces Institute of Pathology. Edn. 3rd.,McG row Hill Book Co., New York, pp 1-46. 
    7. Munson, L. and Moresco, A. (2007). Comparative pathology of mammary gland cancers in domestic and wild animals. Breast Dis. 28: 7-21.
    8. Ngamrojnavanich, N., Sirimongkon, S., Roengsumran, S., Petsom, A., and Kamimura, H. (2003). Inhibition of Na+K+-ATPase activity by (-)-entKaur-16-en-19-oic acid and its derivatives. Planta. Med. 69(6): 555-556 .
    9. Pattanayak, S.P. and Sunita, P. (2008). Anti-tumor potency and toxicology of an Indian ayurvedic plant Hygrophila spinosa. Pharmacol online. 2(1): 361-371.
    10. Roengsumran, S., Musikul, K., Petsom, A., Vilaivan, T., Sangvanich ., Pornpakakul, P.S., Puthong. S., Chaichantipyuth, C., Jaiboon, N. and Chaichit, N. (2002). Croblongifolin, a new anticancer clerodane from Croton oblongifolius. Planta. Med. 68(3): 274-277.
    11. Samy, R.P., Gopalakrishnakone, P. and Ignacimuthu, S. (2006). Anti-tumor promoting potential of luteolin against 7,12-dimethylbenz(a)- anthracene induced mammary tumors in rats. Chem. Biol. Interact. 164: 1-14.
    12. Santhosh Kumar, C.N. (2014). In vitro and in vivo evaluation of anticancer property of Astracanthus longifolia in rats. PhD thesis, Karnataka Veterinary Animal and Fisheries Sciences University, Bidar, India.
    13. Sommit, D., Petsom, A., Ishikawa, T. and Roengsumran, S. (2003). Cytotoxic activity of natural labdanes and their semi-synthetic modified derivatives from Croton oblongifolius. Planta. Med. 69(2): 167-170.
    14. Thun, M.J., Delancey, J.O., Center, M.M., Jemal, A. and Ward, E.M. (2009). The global burden of cancer: Priorities for prevention. Carcinogenesis. 31(1): 100-110.
    15. Tomy, D.V. (2013). Evaluation of anti-oxidant and anti-tumour activities of Croton laevigatus. B.Pharma thesis, RVS College of Pharmaceutical Sciences, Coimbatore, India.
    16. Umadevi, M., Kumar, S.K.P., Bhowmik, D. and Vel, D.S. (2013). Traditionally used anticancer herbs in India. J. Med. Plants Studies. 1(3):56-74.
    17. Viste, J., Myers, S., Singh, B. and Simko, E. 2002. Feline mammary adenocarcinoma: tumor size as a prognostic indicator. Can Vet J. 43(1): 33-37.
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