Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 50 issue 5 (october 2016) : 690-694

Differential expression of Agouti mRNA and its coding protein in viscera of goat with different coat color

Jianqing MA, Yongdong Peng, Xianglong Li*
1<p>College of Animal Science and Technology, Hebei Normal University of Science and Technology,&nbsp;<br /> Qinhuangdao, 066004, Hebei, People&rsquo;s Republic of China.</p>
Cite article:- MA Jianqing, Peng Yongdong, Li* Xianglong (2016). Differential expression of Agouti mRNA and its coding protein inviscera of goat with different coat color . Indian Journal of Animal Research. 50(5): 690-694. doi: 10.18805/ijar.10988.

ASIP has been described to regulate lipid metabolism and acts as a paracrine factor in the regulation of adiposity. The RT-qPCR and Western Blotting was used to investigate the expression level of Agouti mRNA and Agouti signaling protein (ASIP) in viscera (heart, liver, spleen, lung and kidney) of goat with white, brown with gray and black coat color. The main trend of Agouti mRNA abundance represented white goat and liver tissue obviously. The protein was detected in all viscera tissues and it expressed white goat and kidney mainly. However, ASIP differential expresses in different viscera for goats weren’t consistent with the mRNA levels. The differences might be the result from the transcription regulation, translation regulation, protein modified and protein interaction and so on. 

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