Some immunological studies on a new vaccine derived from bacteriophage lysate of Pasteurella multocida in mice

DOI: 10.18805/ijar.v0iOF.7000    | Article Id: B-3258 | Page : 1057-1061
Citation :- Some immunological studies on a new vaccine derived frombacteriophage lysate of Pasteurella multocida in mice .Indian Journal Of Animal Research.2017.(51):1057-1061

Yanglem Pushpa, Sabia Qureshi and Hari Mohan Saxena

hmsaxena@yahoo.com
Address :

Department of Veterinary Microbiology, College of Veterinary Science, GADVASU, Ludhiana-141 004, Punjab, India.

Submitted Date : 15-06-2016
Accepted Date : 8-12-2016

Abstract

The study was undertaken to evaluate the humoral immune response in mice induced by a novel phage lysate vaccine against Hemorrhagic Septicemia developed from Pasteurella multocida grown under iron-restricted conditions and lysed by a bacteriophage. Two groups of mice were immunized with lysate vaccine (LV) and conventional killed HS vaccine (CV) and blood was collected at various days post – immunization (DPI). The serum protein concentration in lysate vaccinated mice (4.675±0.223) was significantly higher (p<0.05) than the conventional vaccinated mice (4.100±0.282) at 150 DPI. The serum globulin levels at 90 DPI and 180 DPI in LV mice (1.330±0.071 and 0.650±0.100) were significantly (p<0.01) higher than the CV mice (0.850±0.084 and 0.366±0.098). The serum IgG levels at 150 DPI and 180 DPI in LV (0.564±0.188 and 0.485±0121) mice were significantly higher (p<0.01) than the CV mice (0.178±0.039 and 0.121±0.026). Although our results in mice are promising, further studies involving bovines are needed.

Keywords

Bacteriophage Hemorrhagic Septicemia Immune response Pasteurella multocida Phage lysate Vaccine.

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