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Indian Journal of Animal Research

  • Chief EditorM. R. Saseendranath

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Research Article

Indian Journal of Animal Research, volume 52 issue 9 (september 2018) : 1324-1330

Sub-acute oxidant and histopathological effects of imidacloprid on kidney of adult female Wistar rats

Archana Lohiya, Vinod Kumar, J.S. Punia
1Department of Veterinary Pharmacology and Toxicology, Lala Lajpat Rai University of Veterinary and Animal sciences, Hisar-125 001, Haryana, India.
Cite article:- Lohiya Archana, Kumar Vinod, Punia J.S. (2018). Sub-acute oxidant and histopathological effects of imidacloprid on kidney of adult female Wistar rats. Indian Journal of Animal Research. 52(9): 1324-1330. doi: 10.18805/ijar.B-3243.

ABSTRACT

Imidacloprid is a neonicotinoid insecticide and has been extensively used as a crop pest and pet flea control programme. In the present study, the effects of imidacloprid on kidney tissue were analyzed in adult female Wistar rats at two dose levels (19 and 38 mg/kg/day) administered orally for 10, 20 and 30 days. Effects were compared with respective control animals administered daily with 2% gum acacia. Different parameters undertaken were kidney weight, levels of cytoplasmic and membrane proteins, oxidative stress parameters viz. activities of SOD, GPx and levels of GSH and MDA and histopathological changes. Imidacloprid at 38 mg/kg dose administered orally for 20 and 30 days significantly increased organ weight and levels of MDA. The activities of enzymes SOD and GPx and levels of GSH were decreased significantly at 38 mg/kg dose administered daily for 30 days. There was significant decrease observed in levels of cytoplasmic and membrane proteins at 38 mg/kg dose administered orally for 20 and 30 days. Histopathological changes in kidney at 38 mg/kg dose in 20 and 30 days group. Therefore, it is concluded that imidacloprid has generated oxidative stress in kidney at 38 mg/kg dose administered orally for 20 and 30 days in adult female Wistar rats. 

REFERENCES

  1. Abdollahi M., Ranjbar A., Shadnia S., Nikfar S., Rezaie A. (2004). Pesticides and oxidative stress: a review. Med Sci Monit 10(6): RA141–RA147.
  2. Abou-Donia M.B., Goldstein L.B., Bulman S., Tu T., Khan W.A., Dechkovskaia A.M., Abdel-Rahman A.A. (2008). Imidacloprid induces neurobehavioral deficits an increases expression of glial fibrillary acidic protein in the motorcortex and hippocampus in offspring rats following in utero exposure, J.Toxicol. Environ. Health 71 119–130.
  3. Anderson M.E., Luo J.L. (1998). Glutathione therapy: from prodrugs to genes, Semin. Liver Dis.18 415–424.
  4. Beutler E., Duron O., Kelly B.M., (1963). Improved Method for the Determination of Blood Glutathione. Journal of Laboratory and Clinical Medicine 61: 882–888.
  5. Booth NH. Inhalant anesthetics. In: Booth NH, McDonald LE, eds. Veterinary pharmacology and therapeutics. 6th ed. Ames, Iowa: Iowa State Univeristy Press, 1988;181–211.
  6. Cordone M.A., Durkin P., (2005). Imidacloprid- human health and ecological risk assessment: final report, submitted by Syracuse Environmental Research Associates, Inc. (SERA TR 05-43-24-03a).
  7. Debnath D., Mandal T.K. (2000). Study of Quinalphos (an environmental oestrogeninsecticide) formulation (ekalux 25E.C.)- induced damage of the testicular tissues and antioxidant defense systems in Sprague–Dawley Albino rats, J.Appl. Toxicol. 20 197–204.
  8. El-Tawil O.S., Abdel-Rahman M.S. (2001). The role of enzyme induction and inhibition on cypermethrin hepatotoxicity, Pharmacol. Res.44 33–40.
  9. Flower K.B., Hoppin J.A., Lynch C.F., Blair A., Knott C., Shore D.L., Sandler D.P. (2004). Cancer risk and parental pesticide application in children of agricultural health study participants, Environ. Health Perspect.112 pp. 631–635.
  10. Giray B., Gurbay A., Hincal F. (2001). Cypermethrin-induced oxidative stress in rat brain and liver is prevented by vitamin E or allopurinol. Toxicol. Lett.,118:139-146. 
  11. Hafeman D.G., Sunde R.A., Hoeskstra W.G. (1974). Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J. Nutr.104: 580-587.
  12. Harper A.J., Rodwell V.M., Meyer P.A. (1977). Review of Physiological Chemistry,16th ed.
  13. Khan S. M., Sobti R.C., and Kataria L. (2005). Pesticide-induced Alteration in Mice Hepatooxidative Status and Protective Effects of Black Tea Extract. Clinica Chimica Acta 358: 131–138.
  14. Kumar S., Occupational exposure associated with reproductive dysfunction. J.Occup. Health 46 (1) (2004) 1–19.
  15. Lopez A., Hernandez A.F., Rodrigo L., Gil F., Pena G., Serrano JL. (2007). Changes in antioxidant enzymes in humans with long-term exposure to pesticides. Toxicol. Lett. 146–53.
  16. Luna L.G. (1968). Manual of histological staining method of arms forces. Institute of pathology, 3 ed. Mc graw hills book company. 
  17. Ohkawa H., Ohishi N., Yagi K. (1972). Assay for Lipid Peroxides in Female rats Tissues by Thiobarbituric Acid Reaction. Analytical Biochemistry 95: 351–358.
  18. Madesh M., Balsubramanian K.A. (1998). Microtitre plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J. Biochemistry and Biophysics 35: pp 184-188.
  19. Mansour S. A., Mossa A.T.H. (2009). Lipid Peroxidation and Oxidative Stress in Rat Erythrocytes Induced by Chlorpyrifos and the Protective Effect of Zinc. Pesticide Biochemistry and Physiology 93: 34–39.
  20. Matsuda K., Buckingham S.D., Klier D., Rauh J.J., Marta G., Sattelle D.B.(2001). Neonicotinoides: insecticides acting on insect nicotinic acetylcholine receptors, Trends Pharmacol. Sci.22: 573–580.
  21. NPIC (National Pesticide Information Centre). 2010. “Imidacloprid Technical Fact Sheet”. Available at: http://npic.orst.edu/factsheets/    imidacloprid.pdf
  22. Panse V.G., Sukhatme D.V. (1978). Statistical method for agricultural workers, 3rd ed., ICAR, New Delhi. 
  23. Paolini M., Sapone A., Gonzalez F.J. (2004). Parkinson’s disease, pesticides andindividual vulnerability, Trends Pharmacol. Sci. 25 124–129.
  24. Parke D.V., Piotrowski J.K. (1996). Glutathione: its role in detoxication of reactive oxygen species and environmental chemicals, Toxicology 4 (1) 1–13.
  25. Rai D. K., Sharma B. ( 2007). Carbofuran-induced Oxidative Stress in Mammalian Brain. Molecular Biotechnology 37: 66–71.
  26. Richardson R. J. (1981). Toxicology of the nervous system. Toxicology principles and practice, [A. L. Reeves (Ed)], Wiley Inter Science Publishers, Toranto.
  27. Salameh P.R., Baldi I., Brochard P., Raherison C., Abi Saleh B., Salamon R.(2003). Respiratory symptoms in children and exposure to pesticides, Eur. Respir. J.22 507–512.
  28. Tomizawa M., Casida J.E. (2000). Imidacloprid, thiacloprid, and their imine derivativesup-regulate the a4b2 nicotinic acetylcholine receptor in M10 cells, Toxicol. Appl. Pharmacol. 169: 114–120.
  29. Tomlin, C. D. S. The Pesticide Manual, A World Compendium, 14th ed.; British Crop Protection Council: Surry, England, 2006; pp 598-599. 
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