Comparative efficacy of cryoprotectant in the lyophilization of pigeon paramyxovirus-1 vaccine
 

DOI: 10.18805/ijar.v0iOF.7656    | Article Id: B-3189 | Page : 173-174
Citation :- Comparative efficacy of cryoprotectant in the lyophilizationof pigeon paramyxovirus-1 vaccine .Indian Journal Of Animal Research.2018.(52):173-174

Mousam Das, Devi Prasad Isore, Chanchal Guha, Ujjwal Biswas, Amaresh Chatterjee

dasmousam001@gmail.com
Address :

Department of Veterinary Microbiology, West Bengal University of Animal &Fishery Sciences, 37, K. B. Sarani, Kolkata- 700037, West Bengal, India.

Submitted Date : 19-02-2016
Accepted Date : 17-09-2016

Abstract

Preparation of live attenuated freeze dried Pigeon Paramyxo Virus-1 (PPMV-1) vaccine selection of proper cryoprotectant which could preserve and protect the virus against injury due to reduction of temperature at the time of lyophilization, was very necessary. PPMV-1 locally isolated lentogenic strain was selected as seed virus which was properly attenuated by serially passaging in 9 to 10 days old embryonated specific pathogen free (SPF) fowl eggs and produced master seed virus and working seed virus. Five per cent lactalbumin hydrolysate with 10% sucrose (LAS) and 1% polyvinyl pyrrolidone (PVP) were used as two different cryoprotectant for lyophilization of working seed virus. After lyophilization value of haemagglutination (HA) titer and embryo infective dose fifty per ml (EID50/ml) were changed and concluded that both LAS and PVP could be used as suitable cryoprotectant where LAS was better than PVP which was first time done in the whole world.

Keywords

Cryoprotectant Lactalbumin hydrolysate Lyophilization Polyvinyl pyrrolidone Sucrose.

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