Nucleotide variability in Beta 2 Microglobulin (b2M) gene and its association with colostral IgG levels in buffaloes (Bubalus bubalis)

DOI: 10.18805/ijar.v0iOF.4566    | Article Id: B-3185 | Page : 51-55
Citation :- Nucleotide variability in Beta 2 Microglobulin (b2M) gene and its association with colostral IgG levels in buffaloes (Bubalus bubalis) .Indian Journal Of Animal Research.2018.(52):51-55

Rajni Chaudhary, Subodh Kumar, H.M. Yathish, A. Sivakumar, Chinmoy Mishra, Amit Kumar, Anuj Chauhan, B. Sivamani and N.R. Sahoo

rajnichaudhary79@gmail.com
Address :

Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Breilly-243 122, Uttar Pradesh, India. 

Submitted Date : 11-02-2016
Accepted Date : 11-06-2016

Abstract

The present study was taken up to find out Beta 2 Microglobulin (b2M) gene variants and their association with IgG concentrations in colostrum of Murrah buffaloes. 40 newly parturated Murrah buffaloes dams were included in the present study. Polymerase chain reaction- single stranded conformational polymorphism (PCR-SSCP) technique was used to explore the polymorphism in b2M gene of buffaloes. The PCR-SSCP analysis and sequencing of different patterns obtained by silver staining of fragments pertaining to exon 1 through 4 and partial introns 1 through 3, revealed 9 SNPs which were further used to prepare the haplotypes. 7 different haplotypes were observed. Indirect ELISA was done to estimate IgG concentrations in colostrum (collected 1 hour before first milking) obtained from buffaloes. IgG levels in colostrum ranged from 11.22 to 185.1 mg/ml with a mean IgG concentration of 51.71 ± 5.99 mg/ml. The effect of different haplotypes was evaluated on IgG concentrations. The least square analysis of variance could not exhibit a significant effect of buffalo dam haplotype on colostral IgG concentration.

Keywords

Beta 2 microglobulin Buffalo Colostrum ELISA FcRn Haplotypes IgG SSCP.

References

  1. Afaq, M., Ashfaque, M., Akhtar, M. and Hayat, B. (1992). Colostral immunoglobulins against Rota and Corona viruses in crossbred cows using Streptavidin Biotin peroxidase enzyme linked immunosorbant assay. Pakistan J. Agric. Sci. 29: 227-29.
  2. Afzal, M., Javed, M. H. and Anjum, A. D. (1983). Calf mortality: Seasonal pattern, age distribution and causes of mortality. Pakistan Vet. J. 3: 30-33.
  3. Agrawal, S., Kumar, S., Chaudhary, R., Yathish, H. M., Kumar, A., Chauhan, A., Sahoo, N. R., Sivamani, B. and Sharma, D. (2015). Identification of nucleotide variability in FCGRT gene in buffalo dams in National Seminar on “Translational Research in B iotechnology for Improving Animal Health and Production”, Bikaner on 7-8 October, 2015 pp: 81.
  4. Agrawal, S., Kumar, S., Chaudhary, R., Kumar, A., Chauhan, A. and Sahoo, N. R. (2016). Polymorphism in intron 2 of FCGRT gene in neonatal buffalo calves and its association with serum IgG concentration in National symposium on “Policy Planning for Livelihood Security through Domestic Animal Biodiversity” on 11-12 Feb, 2016.
  5. Anderson, M., Bocharova, O. V., Makarava, N., Breydo, L., Salnikov V. V. and Baskakov I. V. (2006). Polymorphism and ultrastructural organization of prion protein amyloid fibrils: an insight from high resolution atomic force microscopy. J. Mol. Biol. 358: 580-596.
  6. Asadi, M. H., Mowla, S. J. and Nikpoor, P. (2005). Gene expression profile of CatSper3 and CatSper4 during postnatal development of mouse testis. Iran. Biomed. J. 10: 111-115.
  7. Bassam, B. J., Annoles, G. C. and Greshoff, P. M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Ani. Biochem. 196: 80-83.
  8. Blood, D. C. and Radostits, O. M. (1989). Veterinary Medicine. 7th edn, ELBS, Oxford.
  9. Butler, J. E. (1974). Immunoglobulins of the mammary secretions. In: Larson BL, Smith VR (ed) Lactation: A comprehensive treatise. Vol. III, pp 217, Academic Press, New York.
  10. Chard, T., Parslow, J., Rehmann, T. and Dawnay, A. (1991). The concentrations of transferrin, beta 2-microglobulin and albumin in seminal plasma in relation to sperm count. Fertil. Steril. 55: 211-213.
  11. Chaudhary, R., Kumar, S., Yathish, H. M., Mishra, C., Kumar, A., Chauhan, A., Sahoo, N. R., Sivamani, B. and Sharma, D. (2015). Polymorphism in exon 4 of b2M gene in neonatal buffalo calves and their association with serum IgG concentration in National Seminar on “Translational Research in Biotechnology for Improving Animal Health and Production”, Bikaner on 7-8 October, 2015, pp: 80.
  12. Chaudhary, R., Kumar, S., Yathish, H. M., Sivakumar, A., Mishra, C., Kumar, A., Chauhan, C., Sivamani, B. and Sahoo, N. R. (2016). Identification of SNPs in Beta 2 Microglobulin (b2M) gene and their association with IgG concentration in neonatal buffalo calves. J. Pure Appl. Microbio. 10: 37-42.
  13. Clawson, M. L., Heaton, M. P., Chitko-McKnown, C. G., Fox, J. M., Smith, T. P. L., Snelling, W. M., Keele, J. W. and Laegreid, W. W. (2004). Beta-2-microglobulin haplotypes in U.S. beef cattle and association with failure of passive transfer in newborn calves. Mamm. Genome 15: 228-236.
  14. Dang, A. K., Kapila, S., Purohit, M. and Singh, C. (2009). Changes in colostrum of Murrah buffaloes after calving. Trop. Anim. Health. Prod. 41: 1213-1217.
  15. Duhamel, G. E. and Osbern, B. I. (1984). Neonatal immunity in cattle. Bov. practitioner 19: 71-77.
  16. Harrington, N. P., Surujballi, O. P., Waters, W. R. and Prescott, J. F. (2007). Development and evaluation of a real-time reverse transcription PCR assay for quantification of gamma interferon mRNA to diagnose tuberculosis in multiple animal species. Clinic. Vaccine. Immunol. 14: 1563-1571.
  17. He, X. H., Xu, L. H., Liu, Y. and Zeng, Y. Y. (2004). Cloning of human beta-microglobulin gene and its high expression in Escherichia coli. Sheng Wu Gong Cheng Xue Bao. 20: 99-103.
  18. Jenny, B. F., Cramling, G. E. and Glaze, T. M. (1981). Management factors associated with calf mortality in South Carolina dairy herds. J. Dairy Sci. 64: 2284-2289.
  19. Kuo, T. T., Baker, K., Yoshida, M., Qiao, S. W., Aveson, V. G. and Lencer, W. I. (2010) Neonatal Fc receptor: from immunity to therapeutics. J. Clin. Immunol. 30: 777-789.
  20. Martin, S. W., Wiggins, A. D. (1973). A model of the economic costs of dairy calf mortality. Am. J. Vet. Res. 34: 1027-    1031.
  21. Nikpoor, P., Mowla, S. J., Movahedin, M., Ziaee, S. A. M. and Tiraihi, T. (2004). CatSper gene expression in postnatal development of mouse testis and in subfertile men with deficient sperm motility. Hum. Reprod. 19: 124-128.
  22. Nygard, N. B., Jogensen, C. B., Cirera, S. and Fredholm, M. (2007). Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR. BMC Mol. Bio. 8: 67.
  23. Orita, M., Iwahana, H., Kanazawa, H., Hayashi, K. and Sekiya, T. (1989). Detection of polymorphism of human DNA by gel electrophoresis as single strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA. 86: 2766-2770.
  24. Sambrook, J. and Russell, D. W. (2001). Molecular cloning: A laboratory manual. 3rd edn. Vol. 3, Cold spring harbor Laboratory Press, New York.
  25. Simister, N. E. and Ahouse, J. C. (1996). The structure and evolution of FcRn. Res. Immunol. 147: 333-337.
  26. Sire, J., Colle, A., Pinatel, M. C., Fellous, M. and Manuel, Y. (1978). Beta2-Microglobulin in human seminal fluid. Pathol. Biol. (Paris). 26: 392-394.
  27. Skinner, P. J., Abbassi, H., Chesebro, B., Race, R. E., Reilly, C. and Haase, A. T. (2006). Gene expression alterations in brains of mice infected with three strains of scrapie. BMC Genomics. 7: 114.
  28. Tizard, I. R. (1996). Veterinary immunology: An introduction. 5th edn. Saunders, Philadelphia, PA.
  29. Umoh, J. U. (1982). Relative survival of calves in a university herd in Zaire, Nigeria. British Vet. J. 138: 507-514.
  30. Zhang, H., Gou, J. and Li-min, L. (2001). Determination of IgG in bovine colostrums. China Dairy Industry.
     

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