Indian Journal Of Animal Research

Evaluation of function of RICTOR gene in gga-mir-142-3p knockdowned developing chicken embryo

DOI: 10.18805/ijar.10983    | Article Id: B-3179 | Page : 807-812
Citation :- Evaluation of function of RICTOR gene in gga-mir-142-3pknockdowned developing chicken embryo .Indian Journal Of Animal Research.2017.(51):807-812

Raja Paramasivam, Prabhakar Tembhurne, Maneshkumar Pannerselvam, Vijay Ingle, Nitin Kurkure and Dewananad Kalorey

vetraja86@gmail.com
Address :

Department of Veterinary Microbiology and Animal Biotechnology, Teaching and Research Cell, Nagpur Veterinary College, Nagpur-440 001, Maharashtra, India.

Submitted Date : 3-02-2016
Accepted Date : 4-05-2016
First Online:

Abstract

MicroRNAs (miRNAs) are a large classes of endogenous non coding RNAs of about 18-21 nucleotides long. It regulates the gene expression post-transcriptionally by binding partial or complete complementary sites in 3’ UTRs. The miR-142-3p was highly expressed in all native cell lineages of haematopoietic organs. It has been reported that miR-142-3p was highly expressed in embryonic day 15 and day 20 in spleen and bursa of Fabricius of chicken. The present study was planned to knock down the gga-miR142-3p in chicken embryonic developmental stages for identification of its role in morphogenesis at embryonic spleen and bursa of Fabricius development. Knock down of miR-142-3p in the 14 days old embryonic eggs was carried out by intravenous inoculation of LNA modified miR-142-3p inhibitor. Organs were harvested from the embryos at the embryonic day of 20 and analyzed for gross pathological, histopathological changes and RICTOR gene expressions have been analyzed using SYBR Green qPCR technology. The results indicated that the RICTOR gene was upregulated in all the immune organs and visceral organs except kidney. The over expression of RICTOR gene lead to defective actin reorganization in the cells that will affect the change in the cell structural integrity and cell survival. Over expression of RICTOR gene is associated with increased proliferation of cells along with invasion of tumor cells.

Keywords

Chicken Knock down of ggi-miR-142-3p RICTOR Structural integrity.

References

  1. Bartel, D.P. (2004). Micro RNAs: genomics, biogenesis, mechanism, and function. Cell, 116:281-297.
  2. Bashir, T., Cloninger, C., Artinian, N., Anderson, L., Bernath, A., Holmes, B., Benavides-Serrato, A., Sabha, N., Nishimura, N., Guha, A. and Gera, J. (2012). Conditional astroglial RICTOR overexpression induces malignant glioma in mice. J Exp Med. 209:713–728. 
  3. Delgoffe, G.M., Kole, T.P., Zheng, Y., Zarek, P.E., Matthews, K.L. and Xiao, B. (2009). The mTOR kinase differentially regulates effector and regulatory T cell lineage commitment. Immunity. 30:832-844.
  4. Drury, R.A. and Wallington, E.A. (1980). Carleton’s Histological Techniques, 5th ed, Oxford University Press. London, New York Toronto.1:653-661.
  5. Gu, Y., Lindner, J., Kumar, A., Yuan, W. and Magnuson, M.A. (2011). RICTOR/mTORC2 is essential for maintaining a balance between b-cell proliferation and cell size. Development. 138:3011–3020.
  6. Hicks, J., Tembhurne, P.A. and Liu, H.C. (2008). MicroRNAs expression in chicken embryos. Poult Sci. 87:2335-2343.
  7. Hicks J., Tembhurne, P.A. and Liu, H.C. (2009). Identification of microRNA in the developing chick immune organ. Immunogenetics.61:231-240.
  8. Lee, K., Nam, K.T., Cho, S.H., Gudapati, P., Hwang, Y., Park, D., Potter, R., Chen, J., Volanakis, E. and Boothby, M. (2012). Vital roles of mTOR complex 2 in Notch-driven thymocyte differentiation and leukemia. J Exp Med. 209:713–728. 
  9. Sandhu, S.K., Croce, C.M. and Garzon, R. (2011). Micro RNA expression and function in lymphomas. Adv. Hematol. 10:1155.
  10. Sarbassov, D., Ali, S.M., Kim, D.H., Guertin, D.A., Latek, R.R. and Erdjument B.H. (2004). RICTOR, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor independent pathway that regulates the cytoskeleton. Curr Biol.14:1296-302.
  11. Wienholds, E., Kloosterman,W.P., Miska,E., Alvarez-Saavedra, E., Berezikov, E., de Bruijn, E., Horvitz, H.R., Kauppinen, S. and Plasterk, R.H. (2005). Micro RNA expression in zebra fish embryonic development. Science. 309:310–311.
  12. Zhu, Y., Karla, M., Pires, P., Whitehead, K.J., Olsen, C.D., Wayment, B., Zhang, Y.C., Bugger, H., Ilkun, O., Litwin, S.E., Thomas, G., Kozma, S.C. and Abel, E.D. (2011). Mechanistic target of rapamycin (Mtor) is essential for murine embryonic heart. PLoS ONE. 8:e54221.doi:10.1371/journal.pone.00542
     

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