Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 6 (december 2017) : 1125-1129

Characterization of E. coli pathotypes of bovine and livestock farm environment origin

Trivenee Thakre, Shubhangi Warke, Sumedha Bobade, D.R. Kalorey
1<p>Department of Veterinary Microbiology and Animal Biotechnology,&nbsp;Nagpur Veterinary College, MAFSU, Nagpur-440 006, Maharashtra, India.</p>
Cite article:- Thakre Trivenee, Warke Shubhangi, Bobade Sumedha, Kalorey D.R. (2016). Characterization of E. coli pathotypes of bovine and livestockfarm environment origin . Indian Journal of Animal Research. 51(6): 1125-1129. doi: 10.18805/ijar.v0i0f.3784.

ABSTRACT

In the environment of the farms, feed, fodder and water could be contaminated with fecal material especially which could constitute a reservoir of Enterobacteriaceae bacteria.Total of 80 fecal samples collected from diarrheic calves, normal calves, diarrheic cattle, healthy cattle and shed of cattle were included in this study. Of which 67 (83.75%) isolates were biochemically identified as E.coli. Among 67 E.coli isolates, 12 (17.91%) isolates were of diarrheic cases (10 isolates from 1 month-6 months calf and 2 isolates from diarrheic cow), 51 (76.11%) isolates were of healthy cows (14 isolates from 1 month-6 months calf and 37 isolates from normal cows), 2(2.98%)  from water samples, one isolate (1.49%)each from cow manure and air sample at farm respectively, from cow shed. Eleven (16.41%) out of 67 isolates were found to cause lysis on sheep erythrocytes and 55 (82.089%) out of 67 isolates were found to be biofilm producers on Congo red. Twenty-four (35.82%) isolates out of 67 were positive for bfpA gene, eight (11.94%) for eaeC gene, while five (7.46%) for both the genes of E.coli strain.

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