Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 51 issue 6 (december 2017) : 998-1001

Genetic diversity analysis of the major histocompatibility complex (MHC) region in Indian native chicken breeds and pureline chickens using the LEI0258 microsatellite marker

T. R. Kannaki, M.R. Reddy, K.S. Raja Ravindra, R.N. Chatterjee
1<p>Avian Health Lab, Directorate of Poultry Research,&nbsp;Hyderabad-500 030, Telangana, India.</p>
Cite article:- Kannaki R. T., Reddy M.R., Ravindra Raja K.S., Chatterjee R.N. (2017). Genetic diversity analysis of the major histocompatibility complex (MHC) region in Indian native chicken breeds and pureline chickensusing the LEI0258 microsatellite marker . Indian Journal of Animal Research. 51(6): 998-1001. doi: 10.18805/ijar.v0iOF.6989.

ABSTRACT

The chicken major histocompatibility complex (MHC) plays an important role in immune response and is strongly associated with resistance and susceptibility to many diseases. The microsatellite marker LEI0258 is physically located within MHC, between the BG and BF regions and can identify serologically well-defined MHC haplotypes. In the present study, a total of 150 samples from pureline chicken breeds including Indian native chicken breeds Aseel (20), Kadaknath (45), White Leghorn (IWI-20; IWK-23), Dahlem red (DR-20), Broiler (PB1-22) was investigated by using LEI0258 microsatellite marker. Overall 22 differently sized alleles (182-552 bp) were identified in all the five lines. Our results indicate that Indian native chicken breeds Aseel (Na=10) and Kadaknath (Na=11) harbored more alleles compared to those of pureline populations (Na=5-6). Analysis of Hardy-Weinberg’s equilibrium (HWE) revealed that two populations IWI and Kadaknath deviated from the HWE (P<0.001). LEI0258 microsatellite based MHC haplotyping would be useful in selecting birds for breeding programs and also in genetic resource conservation.

REFERENCES


  1. Bacon, L.D. (1987). Influence of the major histocompatibility complex on disease resistance and productivity. Poult. Sci., 66: 802-811.

  2. Bacon, L.D., Hunt, H.D. and Cheng. H. H. (2001). Genetic resistance to Marek’s disease. Curr. Top. Microbiol. Immunol., 255: 121-141.

  3. Baelmans, R.,. Parmentier, H.K., Nieuwland, M.G., Dorny, P. and Demey, F. (2005). Serological screening for MHC (B)-    polymorphism in indigenous chickens. Trop. Anim. Health Prod., 37: 93–102. 

  4. Briles, W.E. and Briles, R.W. (1982). Identification of haplotypes of the chicken major histocompatibility complex (B). Immunogenetics, 15: 449-459.

  5. Briles, W.E., Stone, H.A. and Cole, R.K. (1977). Marek’s disease: effects of B histocompatibility alloalleles in resistant and susceptible chicken lines. Science, 195: 193-195.

  6. Fulton, J,E., Juul-Madsen, H.R., Ashwell, C.M., McCarron, A.M., Arthur, J.A., O’Sullivan, N.P. and Taylor, R.L. Jr. (2006). Molecular genotype identification of the Gallus gallus major histocompatibility complex. Immunogenetics, 58: 407-421.

  7. Haunshi, S. and D. Sharma (2002). Immunocompetence in native and exotic chicken population and their crosses developed for rural farming system. Indian J. Poult. Sci., 37: 10-15.

  8. Haunshi, S., Niranjan, M., Shanmugam, M., Padhi, M.K., Reddy, M.R., Sunitha, R., Rajkumar, U. and Panda, A.K. (2011). Characterization of two Indian native chicken breeds for production, egg and semen quality, and welfare traits. Poult. Sci., 90: 314-320. 

  9. Heller, E.D., Uni, Z. and Bacon, L.D. (1991). Serological evidence for major histocompatibility complex (B complex) antigens in broilers selected for humoral immune response. Poult. Sci., 70: 726-732.

  10. Izadi, F., Ritland, C. and Cheng, K. M. (2011). Genetic diversity of the major histocompatibility complex region in commercial and noncommercial chicken flocks using the LEI0258 microsatellite marker. Poult. Sci., 90: 2711-2717.

  11. Kaufman, J. (2008). The avian MHC. In: Avian immunology. Elsevier, London, pp., 159–242.

  12. Kroemer, G., Bernot, A., Béhar, G., Chaussé, A.M., Gastinel, L.N., Guillemot, F., Park, I., Thoraval, P., Zoorob, R. and Auffray, C. (1990). Molecular genetics of the chicken MHC: current status and evolutionary aspects. Immunol Rev., 113:119-145.

  13. Kundu, A., Singh, D. P., Mohapatra, S.C., Dash, B.B., Moudgal, R.P. and Bisht, G.S. (1999). Antibody response to sheep erythrocytes in Indian native vis-à-vis imported breeds of chickens. Br. Poult. Sci., 40: 40–43.

  14. Lamont, S. J. (1989). The chicken major histocompatibility complex in disease resistance and poultry breeding. J. Dairy Sci., 72:1328-1333.

  15. Lamont, S. J., Bolin, C. and Cheville, N. (1987). Genetic resistance to fowl cholera is linked to the major histocompatibility complex. Immunogenetics, 25: 284-289.

  16. Lima-Rosa, C. AdV., Canal, C.W., Fallavena, P.R.V., Freitas, L.Bd. and Salzano, F.M. (2005). LEI0258 microsatellite variability and its relationship to B–F haplotypes in Brazilian (blue-egg Caipira) chickens. Genet. Mol. Biol., 28:386–389.

  17. Lwelamira, J., Kifaro, G.C. and Gwakisa, P.S. (2009). Genetic parameters for body weights, egg traits and antibody response against Newcastle disease virus (NDV) vaccine among two Tanzania chicken ecotypes. Trop. Anim. Health Prod., 41:51–59.

  18. McConnell, S. K., Dawson, D.A., Wardle, A. and Burke, T. (1999). The isolation and mapping of 19 tetranucleotide microsatellite markers in the chicken. Anim. Genet., 30:183-189.

  19. Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 76: 379–390.

  20. Nikbakht, G., Esmailnejad, A. and Barjesteh, N. (2013). LEI0258 microsatellite variability in Khorasan, Marandi, and Arian chickens. Biochem. Genet., 51: 341–349.

  21. Sambrook, J. and Russell, D.W. (2001). Molecular cloning; a laboratory manual, vol 1, 3rd edn. Cold Spring Harbor Laboatory Press. New York.

  22. Schou, T.W., Permin, A., Juul-Madsen, H,R., Sorensen, P., Labouriau, R., Nguyen, T.L., Fink, M. and Pham, S.L. (2007). Gastrointestinal helminths in indigenous and exotic chickens in Vietnam: association of the intensity of infection with the major histocompatibility complex. Parasitology, 134:561–573.

  23. Weir, B.S. (1996). Genetic data analysis II: methods for discrete population genetic data. Sinauer Associates, Sunderland.

  24. Zekarias, B., Ter Huurne, A.A., Landman, W.J., Robel, J.M., Pol, J.M. and Gruys, E. (2002). Immunological basis of differences in disease resistance in the chicken. Vet. Res., 33: 109–125.

     
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)