Indian Journal Of Animal Research

Comparative evaluation of conventional staining method and enzyme linked immunosorbent assay kits for the detection of bovine cryptosporidiosis
 

DOI: 10.18805/ijar.v0iOF.9120    | Article Id: B-3130 | Page : 916-921
Citation :- Comparative evaluation of conventional staining method and enzyme linked immunosorbent assay kits for the detection of bovine cryptosporidiosis .Indian Journal Of Animal Research.2017.(51):916-921

B. J. Thakre, J.B. Solanki, N. Kumar and A. Vargese 

niruvet@gmail.com
Address :

Department of Parasitology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari-396 450, Gujarat, India.

Submitted Date : 7-10-2015
Accepted Date : 19-03-2016
First Online:

Abstract

Out of 428 faecal 178 (41.59%) animals were found positive for Cryptosporidium parvum oocysts in the bovine faeces in modified Ziehl-Neelsen (mZN) staining and/ or commercial plate and dipstick- enzyme linked immunosorbent assay (ELISA) kits (Cypress diagnostics, Langdorp, Belgium) with statistically non-significant difference in the occurrence rate in cattle and buffalo calves. Seasonal prevalence difference was statistically significant in cattle calves while non-significant in buffalo calves, respectively. The highest overall prevalence was recorded during rainy season (45.15%) and almost same per cent during winter and summer. Prevalence of 67.26, 37.11, 30 and 17.65% was recorded in calves aged below 1 month, 1-3 months, 4-8 months and 9-12 months, respectively (p<0.05). There was no significant difference in the age group prevalence between cattle and buffalo calves. Both sexes of bovines are equally susceptible to the cryptosporidiosis (p>0.05). The prevalence of cryptosporidiosis was 59.54 and 29.41% for diarrhoeic and non-diarrhoeic samples, respectively (p<0.05). Both diarrhoeic and non-diarrhoeic groups of calves aged between 1-30 days were equally susceptible to the infection of Cryptosporidium spp. Almost same per cent prevalence of the infection was observed in dairy calves reared in organized (40.76%) and unorganized farms (42.21%) and the difference was non-significant in both cattle and buffalo calves. Highest prevalence of cryptosporidiosis was found in HF cross calves. The tests failed to detect the oocysts in infected soil samples. There was highly significant difference in the prevalence of cryptosporidiosis estimated by plate/ dipstick-ELISA and mZN staining with highest sensitivity and specificity in plate-ELISA. 

Keywords

Bovine Cryptosporidium spp Diarrhoea ELISA Modified Ziehl Neelsen staining.

References

  1. Abeywardena, H.; Jex, A.R. and Gasser, R.B. (2015). A perspective on Cryptosporidium and Giardia, with an emphasis on bovines and recent epidemiological findings. Adv. Parasitol. 88: 243-301.
  2. Ahamed ,I.; Yadav, A.; Katoch, R.; Godara, R.; Saleem, T. and Nisar, N. A. (2015) Prevalence and analysis of associated risk factors for Cryptosporidium infection in lambs in Jammu district. J. Parasit. Dis. 39(3): 414-417. 
  3. Amer, S.; Zidan, S.; Feng, Y.; Adamu, H.; Li, N. and Xiao, L. (2013). Identity and public health potential of Cryptosporidium spp. in Water Buffalo calves in Egypt. Vet. Parasitol. 191(1-2): 123-127. 
  4. Ayinmode, A. B. and Fagbemi, B. O. (2010). Prevalence of Cryptosporidium infection in cattle from southern Nigeria. Vet. Archiv. 80(6): 723-731. 
  5. Bhat, S. A.; Juyal, P. D.; Singh, N. K. and Singla, L. D. (2013). Coprological investigation on neonatal bovine cryptosporidiosis in Ludhiana, Punjab. J. Parasit. Dis. 37(1): 114-117.
  6. Björkman, C.; Lindström, L.; Oweson, C.; Ahola, H.; Troell, K. and Axén, C. (2015). Cryptosporidium infections in suckler herd beef calves. Parasitol. 142(8): 1108-1114. 
  7. Cho, Y. I.; Sun, D.; Cooper, V.; Dewell, G.; Schawartz, K. and Yoon, K. J. (2012). Evaluation of a commercial rapid test kit for detecting bovine enteric pathogens in feces. J. Vet. Diagnos. Inv. 24(3): 559-562.
  8. Dubey, J. P.; Fayer, R. and Rao, J. R. (1992). Cryptosporidium oocysts in faeces of water buffalo and zebu calves in India. J. Vet. Parasitol. 6: 55-56.
  9. Galuppi, R.; Piva, S.; Castagnetti, C.; Sarli, G.; Iacono, E. Fioravanti, M.L. and Caffara, M. (2016). Cryptosporidium parvum: From foal to veterinary students. Vet. Parasitol. 219: 53-56.
  10. Hawash, Y.; Ghonaim, M. M. and Al-Hazmi, A. S. (2015). Internal amplification control for a cryptosporidium diagnostic PCR: construction and clinical evaluation. Korean J. Parasitol. 53(2): 147-154.
  11. Helmy, Y. A.; Krücken, J.; Nöckler, K.; von Samson-Himmelstjerna, G. and Zessin, K.H. (2014). Comparison between two commercially available serological tests and polymerase chain reaction in the diagnosis of Cryptosporidium in animals and diarrhoeic children. Parasitol. Res. 113(1): 211-216. 
  12. Kaupke, A. and Rze¿utka, A. (2015). Emergence of novel subtypes of Cryptosporidium parvum in calves in Poland. Parasitol. Res. 114(12): 4709-4716.
  13. Kumar, D.; Sreekrishnan, R. and Das, S. S. (2004). Cryptosporidiosis in man and animals in puducherry. Indian J. Anim. Sci. 74: 261-263.
  14. Mallinath, R. H. K.; Chikkachowdappa, P. G.; Gowda, A. J. K. and D’souza, P. E. (2009). Studies on the prevalence of cryptosporidiosis in bovines in organized dairy farms in and around Bangalore, South India. Vet. Archiv. 79: 461-470.
  15. Masood, S.; Maqbool, A.; Anjum, A. A.; Rashid, M. L. and Choudhary, Z. I. (2013). Prevalence of Cryptosporidium oocysts in bovine at different livestock farms by conventional microscopic and molecular techniques. The J. Ani. Sci. 23(6): 1588-1594.
  16. Maurya, P. S.; Rakesh, R. L.; Pradeep, B.; Kumar, S.; Kunda, K.; Garg, R.; Ram, H.; Kumar, A. and Banerjee, P. S. (2013). Prevalence and risk factors associated with Cryptosporidium spp. infection in young domestic livestock in India. Trop. Ani. Health Prod. 45(4): 941-946.
  17. Mirhashemi, M. E.; Zintl, A.; Grant, T.; Lucy, F. E.; Mulcahy, G. and Waal, T.D. (2015). Comparison of diagnostic techniques for the detection of Cryptosporidium oocysts in animal samples. Exp. Parasitol. 151-152: 14-20. 
  18. Mirhashemi, M.E.; Zintl, A.; Grant, T.; Lucy, F.; Mulcahy, G. and Waal, T.D. (2016). Molecular epidemiology of Cryptosporidium species in livestock in Ireland. Vet. Parasitol. 216: 18-22.
  19. Mohammad, H. R.; Gowhari, M. A. and Mohammad, M. (2013). Comparison of capture ELISA and modified Ziehl-    Neelsen for detection of Cryptosporidium parvum in faeces of camel (Camelus dromedarius) in Iran. Sci. Parasitol. 14(3): 147-152.
  20. O’hara, S. P. and Chen X. M. (2011). The cell biology of Cryptosporidium infection. Microbes Infec. 13: 721-730.
  21. Office International des Epizootics (OIE). (2008). Cryptosporidiosis. In: Terrestrial manual chapter 2.9.4, pp 1192-1215.
  22. Olson, M. E.; Thorlakson, C. L.; Deselliers, L.; Morck, D. W. and Mcallister, T. A. (2004). Giardia and Cryptosporidium in Canadian farm animals. Vet. Parasitol. 68: 375-381.
  23. Omoruyi, B. E.; Nwodo, U. U.; Udem, C. S. and Okonkwo, F. O. (2014). Comparative diagnostic techniques for Cryptosporidium Infection. Molecules, 19: 2674-2683.
  24. Qi, M.; Cai, J.; Wang, R.; Li, J.; Jian, F.; Huang, J.; Zhou, H. and Zhang, L. (2015). Molecular characterization of Cryptosporidium spp. and Giardia duodenalis from yaks in the central western region of China. BMC Microbiol. 15: 108/ 1-7.
  25. Rajkhowa, S.; Rajkhowa, C. and Hazarika, G. C. (2006). Prevalence of Cryptosporidium parvum in mithuns (Bos frontalis) from India. Vet. Parasitol. 142: 146-149. 
  26. Rau, A. K. (2012). Cryptosporidiosis in pre-weaned calves observation in Germany and Portugal. Dis. Mast. Med. Vet. 30: 62-65.
  27. Rekha, H. K.; Puttalakshmamma, G. C.; Ananda, K. J. and D’souza, P. E. (2009). Studies on the prevalence of cryptosporidiosis in bovines in organized dairy farms in and around Bangalore, South India. Vet. Arhiv. 79(5): 461-470.
  28. Romero-Salas, D.; Alvarado-Esquivel, C.; Cruz-Romero, A.; Aguilar-Domínguez, M.; Ibarra-Priego, N.; Merino-Charrez, J.O.; Pérez de León, A. A. and Hernández-Tinoco, J. (2016). Prevalence of Cryptosporidium in small ruminants from Veracruz, Mexico. BMC Vet. Res. 12:14/ 1-6.
  29. Roy, S. S.; Saankar, S.; Batabyal, S.; Pramanik, A. K. and Das, P. (2006). Observations on the epidemiology of bovine cryptosporidiosis in india. Vet. Parasitol. 141: 330-333.
  30. Shobhamani, B. and Singari N. A. (2006). Prevalence of cryptosporidiosis in calves in and around Tirupati. Indian Vet. J. 83: 92-93.
  31. Sivajothi, S.; Sudhakara, R. B. and Rayulu, V. C. (2014). Cryptosporiodiosis in neonatal dairy calves. Adv. Appl. Sci. Res. 5(1): 74-76.
  32. Vastert-koop, D.; Brinkman, M.; Wilke, H. and Mulder B. (2012). Diagnosis of Cryptosporidium parvum with microscopy, striptest, ELISA and real- time PCR. Regional Public Health Laboratory, Enschede, The Netherlands. Abstract: P857.
  33. Venu, R.; Latha, B. R.; Abdul Basith, S.; Dhinakar, G.; Sreekumar, C. and Raman, M. (2012). Molecular prevalence of Cryptosporidium spp. in dairy calves in Southern states of India. Vet. Parasitol. 188: 19-24.
  34. Venu, R.; Latha, B.R.; Basith, S. A.; Sreekumar, C.; Raj, G. D. and Raman, M. (2013). Factors influencing on prevalence of Cryptosporidium infection in south Indian dairy calves. J. Parasit. Dis. 37(2): 168-172. 
  35. Yadav, A.; Katoch, M.; Aggrawal, R. and Kalha, R. (2011). Epidemiology of cryptosporidiosis in bovine calves of Jammu. Proceeding of 21st National Congress of IAAVP, 5-7 January, p, 46.
     

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