Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 51 issue 3 (june 2017) : 420-422

Seasonal effect on viability and morphology of spermatozoa in Karan Fries (Holstein Friesian×Tharparkar) bulls under tropical climatic condition

Simson Soren*, S.V. Singh, R.C. Upadhyay, Pawan Singh, Sunil Kumar
1<p>Climate Resilient Livestock Research Centre,&nbsp;Dairy Cattle Physiology, ICAR-NDRI, Karnal-132 001, India.</p>
Cite article:- Soren* Simson, Singh S.V., Upadhyay R.C., Singh Pawan, Kumar Sunil (2016). Seasonal effect on viability and morphology of spermatozoa in Karan Fries (Holstein Friesian&times;Tharparkar) bulls under tropical climatic condition . Indian Journal of Animal Research. 51(3): 420-422. doi: 10.18805/ijar.9638.

Availability of morphologically normal and higher percentage of live spermatozoa throughout the year is highly demanded to fulfilment the demand of dairy cattle. There may be deterioration of semen quality during summer season in crossbred bulls in tropical climatic condition. Therefore the present study was designed to observe the seasonal effect on viability and morphology of spermatozoa in Karan Fries bulls. Five adult healthy bulls (n=5) were selected from Animal Breeding Research Centre (ABRC) of ICAR-NDRI, Karnal, Haryana (India). Six ejaculates from each bull were collected using artificial vagina (42-45 °C) during winter (December to Middle February), spring (Middle February to April), hot-dry (May to June) and hot- humid (July to August) seasons. Immediately of collection, the ejaculates were placed in a water bath (37 °C). Eosin-nigrosin vital stain was used for assessing live, dead and sperm abnormalities. The percentage of live spermatozoa were significantly (p<0.05) lowered during hot humid season and the major abnormalities were significantly (p<005) higher during hot-dry (8.66 ± 0.22 %) and hot-humid (10.74 ± 0.18 %) seasons. It can be concluded that hot-dry and hot-humid seasons are stressful to Karan Fries bulls. Therefore, sufficient measures should be taken to ameliorate the effect of heat stress on Karan Fries bulls.


  1. Bhakat, M., Mohanty, T. K., Gupta, A. K. and Abdullah, M. (2014). Effect of season on semen quality of crossbred (Karan Fries) bulls. Adv. Anim. Vet. Sci. 2: 632-637. doi http://dx.doi.org/10.14737/journal.aavs/2014/2.11.632.637.

  2. Chacur, M. G. M., Mizusaki, K. T. and Garielfilho, L. R. A. (2013). Seasonal effects on semen and testosterone in Zebu and Taurine Bulls. Acta Scientiae Veterinaria. 41: 1110. 

  3. Chaudhry, M.Z., McDowell, R.E. and Shah, S. K. (1990). Productive and reproductive performance of three generations of Holstein × Sahiwal. 11TH annual Report, L.P.R.I., Bahadarnagar, Okara Pakistan. pp: 29-39.

  4. Fiaz, M., Usmani, R. H., Abdullah, M. and Ahmad, T. (2010). Evaluation of semen quality of Holstein Friesian and Jersey bulls maintained under subtropical environment. Pak Vet J. 30: 75-78. 

  5. Miah, A. G., Salmaand, U., Hossain, M. M. (2004). Factors Influencing Conception Rate of Local and Crossbred Cows in Bangladesh. Int. J. Agri. Biol. 6 : 797–801. 

  6. Mieusset, R., Quintana-Casares, P., Sanchez-Partida, L. G., Sowrbutts, S. F., Zupp, J. L. and Setchell, B. P. (1992). Effects of heating the testis and epididymites of ram by scrotal insulation on fertility and embryo mortality in ewes inseminated with frosen semen. Journal of Reproduction and Fertility. 94:337-344.

  7. Salah, M.S., El-Noutyand, F. D., Al-Hajri, M. R. (1992). Effect of season on seminal characteristic of Holstein bulls under semi-arid environment II sperm abnormalities. Asian Australas. J. Anim. Sci. 5: 449-454. 

  8. Shukla, M. K., Sujit Saha, S. K., Saxena, R. B., Singh Tiwari, R. and Mishra, G. K. (2010). Effect of season on sperm morphology in different breeds of cattle and Murrah buffaloes. Indian J. Field vet. 6: 1-4. 

  9. Vilakazi, D. M. and Webb, E. C. (2004). Effect of age and season on sperm morphology of Friesland bulls at an artificial insemination centre in South Africa. S. Afr. J. Anim. Sci. 34: 62-69.

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