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Research Article

volume 49 issue 5 (october 2015) : 595-598,   Doi: 10.18805/ijar.5568

Molecular characterization and polymorphism detection in HSPB6 gene in Sahiwal cattle

R
Rakesh Kumar
I
I.D. Gupta
A
Archana Verma
N
Nishant Verma
A
Ankit Magotra
M
M.R. Vineeth
1Dairy Cattle Breeding, ICAR- National Dairy Research Institute,Karnal-132001, India.
Cite article:- Kumar Rakesh, Gupta I.D., Verma Archana, Verma Nishant, Magotra Ankit, Vineeth M.R. (2025). Molecular characterization and polymorphism detection in HSPB6 gene in Sahiwal cattle. Indian Journal of Animal Research. 49(5): 595-598. doi: 10.18805/ijar.5568.

ABSTRACT

The present study was undertaken with the objectives of molecular characterization and detection of genetic polymorphisms in the HSPB6 gene of Sahiwal cattle. A total of 100 Sahiwal cattle maintained at LRC, NDRI, Karnal (Haryana) were included in the study. HSPB6 gene has been mapped on Bos taurus autosome 18 (BTA 18) and spans nearly 2620 bp comprising 3 exons and 2 introns. Four sets of forward and reverse gene-specific oligonucleotide primers of HSPB6 gene were designed using Primer3 software and amplicon size of 529 bp, 460 bp, 670 bp and 502 bp were ascertained. On the basis of comparative sequence analysis, total three polymorphic loci (SNPs) were detected at locus G436A, A2152G and A2417T in HSPB6 gene as compare to Bos taurus (NCBI GenBank AC_000175.1). Out of these three SNPs, two (A2152G & A2417T) were found in 3´-UTR region and third one (G436A) was detected in intronic region 1. However, monomorphic pattern was found in exon 2 of HSPB6 gene in Sahiwal cattle. Further research need be directed to find out the association of allelic variants in major heat shock protein genes to establish markers for the future genetic improvement of livestock through marker assisted selection.

REFERENCES


  1. Arrigo, A.P. (2007). The cellular networking of mammalian Hsp27 and its functions in the control of protein folding, redox state and apoptosis. Adv. Exp. Med. Biol., 594:14–26.

  2. Beall, A.C. and Kato, K. (1997). Cyclic nucleotide-dependent vasorelaxation is associated with the phosphorylation of a small heat shock-related protein. J. Biol. Chem., 272:11283–11287.

  3. Brown, J.A.H., Reiter, S.T., Brown, M.A., Johnson, Z.B., Nabhan, I.A., Lamb, M.A., Starnes, A.R. and Rosenkrans, J.C.F. (2010). Effects of heat shock protein-70 Gene and forage system on milk yield and composition of beef cattle. The Professional Anim. Scientist., 26:398

  4. Bryantsev, A.L., Kurchashova, S.Yu., Golyshev, S.A., Polyakov, V.Yu., Wunderink, H.F., Kanon, B., Budagova, K.R., Kabakov, A.E. and Kampinga, H.H. (2007). Regulation of stress-induced intracellular sorting and chaperone function of Hsp27 (HspB1) in mammalian cells. Biochem. J., 407:407–417.

  5. Cross, B.E. and O’ Dea, H.M. (2007). Expression of small heat shock-related protein 20 (HSP20) in rat myometrium is markedly decreased during late pregnancy and labour. Reproduction., 133: 807–817.

  6. Ellis, R.J. (2006). Protein misassembly: macromolecular crowding and molecular chaperones. Harbor Laboratory Press, Cold Spring Harbor, New York.

  7. Fan, G. C. and Ren, X. (2005). Novel cardio protective role of a small heat-shock protein, Hsp20, against ischemia/ reperfusion injury. Circulation., 111:1792–1799.

  8. Garcia-Ranea., J.A, Mirey, G., Camonis, J. and Valencia, A. (2002). p23 and HSP20/alpha-crystallin proteins define a conserved sequence domain present in other eukaryotic protein families. FEBS Lett., 529: 162–167

  9. Garrido, S., Gurbuxani, L., Ravagnan, G. and Kroemer. (2001). Heat shock proteins: endogenous modulators of apoptotic cell death. Biochem. Biophys. Res. Commun., 286: 433–442.

  10. Kato, K., Goto, S., Inaguma, Y., Hasegawa, K., Morishita, R. and Asano, T. (1994). Purification and characterization of a 20-kDa protein that is highly homologous to alpha B crystallin. J. Biol. Chem., 269: 15302–9.

  11. Komalavilas, P. and Penn, R.B. (2008). The small heat shock-related protein, HSP20, is a cAMP-dependent protein kinase substrate that is involved in airway smooth muscle relaxation. Am. J .Physiol. Lung Cell Mol Physiol., 294.

  12. Pipkin, W. and Johnson, J.A. (2003). Localization, macromolecular associations, and function of the small heat shock- related protein HSP20 in rat heart. Circulation., 107:469–476.

  13. Rembold, C.M. and Connor, M. (2001). Selected contribution: HSP20 phosphorylation in nitroglycerin and forskolin induced sustained reductions in swine carotid media tone. J Appl. Physiol., 91:1460–1466.

  14. Sambrook, J. and Russell, D.W. (1989) Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

  15. Santoro, M.G. (2000). Heat shock factors and the control of the stress response. Biochemical pharmacology., 59: 55–63.

  16. Tessier, D.J. and Komalavilas, P. (2004). Transduction of peptide analogs of the small heat shock-related protein HSP20 inhibits intimal hyperplasia. J .Vasc. Surg., 40:106–114.

  17. Woodrum, D.A. and Brophy, C.M. (1999). Phosphorylation events associated with cyclic nucleotide dependent inhibition of smooth muscle contraction. Am. J. Physiol., 277: 931–939.

  18. Yeh, F.C., Yang, R. and Bozle, T. (1999). POPGENE version 1.31, MS Windows-based freeware for population genetic analysis. University of Alberta and Center for International Forestry Research.
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    Research Article

    volume 49 issue 5 (october 2015) : 595-598,   Doi: 10.18805/ijar.5568

    Molecular characterization and polymorphism detection in HSPB6 gene in Sahiwal cattle

    R
    Rakesh Kumar
    I
    I.D. Gupta
    A
    Archana Verma
    N
    Nishant Verma
    A
    Ankit Magotra
    M
    M.R. Vineeth
    1Dairy Cattle Breeding, ICAR- National Dairy Research Institute,Karnal-132001, India.
    Cite article:- Kumar Rakesh, Gupta I.D., Verma Archana, Verma Nishant, Magotra Ankit, Vineeth M.R. (2025). Molecular characterization and polymorphism detection in HSPB6 gene in Sahiwal cattle. Indian Journal of Animal Research. 49(5): 595-598. doi: 10.18805/ijar.5568.

    ABSTRACT

    The present study was undertaken with the objectives of molecular characterization and detection of genetic polymorphisms in the HSPB6 gene of Sahiwal cattle. A total of 100 Sahiwal cattle maintained at LRC, NDRI, Karnal (Haryana) were included in the study. HSPB6 gene has been mapped on Bos taurus autosome 18 (BTA 18) and spans nearly 2620 bp comprising 3 exons and 2 introns. Four sets of forward and reverse gene-specific oligonucleotide primers of HSPB6 gene were designed using Primer3 software and amplicon size of 529 bp, 460 bp, 670 bp and 502 bp were ascertained. On the basis of comparative sequence analysis, total three polymorphic loci (SNPs) were detected at locus G436A, A2152G and A2417T in HSPB6 gene as compare to Bos taurus (NCBI GenBank AC_000175.1). Out of these three SNPs, two (A2152G & A2417T) were found in 3´-UTR region and third one (G436A) was detected in intronic region 1. However, monomorphic pattern was found in exon 2 of HSPB6 gene in Sahiwal cattle. Further research need be directed to find out the association of allelic variants in major heat shock protein genes to establish markers for the future genetic improvement of livestock through marker assisted selection.

    REFERENCES


    1. Arrigo, A.P. (2007). The cellular networking of mammalian Hsp27 and its functions in the control of protein folding, redox state and apoptosis. Adv. Exp. Med. Biol., 594:14–26.

    2. Beall, A.C. and Kato, K. (1997). Cyclic nucleotide-dependent vasorelaxation is associated with the phosphorylation of a small heat shock-related protein. J. Biol. Chem., 272:11283–11287.

    3. Brown, J.A.H., Reiter, S.T., Brown, M.A., Johnson, Z.B., Nabhan, I.A., Lamb, M.A., Starnes, A.R. and Rosenkrans, J.C.F. (2010). Effects of heat shock protein-70 Gene and forage system on milk yield and composition of beef cattle. The Professional Anim. Scientist., 26:398

    4. Bryantsev, A.L., Kurchashova, S.Yu., Golyshev, S.A., Polyakov, V.Yu., Wunderink, H.F., Kanon, B., Budagova, K.R., Kabakov, A.E. and Kampinga, H.H. (2007). Regulation of stress-induced intracellular sorting and chaperone function of Hsp27 (HspB1) in mammalian cells. Biochem. J., 407:407–417.

    5. Cross, B.E. and O’ Dea, H.M. (2007). Expression of small heat shock-related protein 20 (HSP20) in rat myometrium is markedly decreased during late pregnancy and labour. Reproduction., 133: 807–817.

    6. Ellis, R.J. (2006). Protein misassembly: macromolecular crowding and molecular chaperones. Harbor Laboratory Press, Cold Spring Harbor, New York.

    7. Fan, G. C. and Ren, X. (2005). Novel cardio protective role of a small heat-shock protein, Hsp20, against ischemia/ reperfusion injury. Circulation., 111:1792–1799.

    8. Garcia-Ranea., J.A, Mirey, G., Camonis, J. and Valencia, A. (2002). p23 and HSP20/alpha-crystallin proteins define a conserved sequence domain present in other eukaryotic protein families. FEBS Lett., 529: 162–167

    9. Garrido, S., Gurbuxani, L., Ravagnan, G. and Kroemer. (2001). Heat shock proteins: endogenous modulators of apoptotic cell death. Biochem. Biophys. Res. Commun., 286: 433–442.

    10. Kato, K., Goto, S., Inaguma, Y., Hasegawa, K., Morishita, R. and Asano, T. (1994). Purification and characterization of a 20-kDa protein that is highly homologous to alpha B crystallin. J. Biol. Chem., 269: 15302–9.

    11. Komalavilas, P. and Penn, R.B. (2008). The small heat shock-related protein, HSP20, is a cAMP-dependent protein kinase substrate that is involved in airway smooth muscle relaxation. Am. J .Physiol. Lung Cell Mol Physiol., 294.

    12. Pipkin, W. and Johnson, J.A. (2003). Localization, macromolecular associations, and function of the small heat shock- related protein HSP20 in rat heart. Circulation., 107:469–476.

    13. Rembold, C.M. and Connor, M. (2001). Selected contribution: HSP20 phosphorylation in nitroglycerin and forskolin induced sustained reductions in swine carotid media tone. J Appl. Physiol., 91:1460–1466.

    14. Sambrook, J. and Russell, D.W. (1989) Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

    15. Santoro, M.G. (2000). Heat shock factors and the control of the stress response. Biochemical pharmacology., 59: 55–63.

    16. Tessier, D.J. and Komalavilas, P. (2004). Transduction of peptide analogs of the small heat shock-related protein HSP20 inhibits intimal hyperplasia. J .Vasc. Surg., 40:106–114.

    17. Woodrum, D.A. and Brophy, C.M. (1999). Phosphorylation events associated with cyclic nucleotide dependent inhibition of smooth muscle contraction. Am. J. Physiol., 277: 931–939.

    18. Yeh, F.C., Yang, R. and Bozle, T. (1999). POPGENE version 1.31, MS Windows-based freeware for population genetic analysis. University of Alberta and Center for International Forestry Research.
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