Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 2 (april 2017) : 316-321

Evaluation of ERIC and (GTG)5 fingerprinting to differentiate various serotypes of Salmonella from diverse sources

Priscilla Kerketta*, Rajesh Kumar Agarwal, Blessa Sailo, P. Pavan Kumar, Rashmi Goyal, Joy L. Kataria, Ashok Kumar
1<p>Division of Veterinary Public Health,&nbsp;Indian Veterinary Research Institute, Izatnagar-243 122, (UP), India.</p>
Cite article:- Kerketta* Priscilla, Agarwal Kumar Rajesh, Sailo Blessa, Kumar Pavan P., Goyal Rashmi, Kataria L. Joy, Kumar Ashok (2016). Evaluation of ERIC and (GTG)5 fingerprinting to differentiatevarious serotypes of Salmonella from diverse sources . Indian Journal of Animal Research. 51(2): 316-321. doi: 10.18805/ijar.v0i0f.3807.

ABSTRACT

A total of 19 Salmonella strains isolated from diverse sources were subjected to DNA fingerprinting by repetitive sequence PCR using ERIC and (GTG)5 primers for the discrimination of Salmonella enterica isolates at the serotype level. Fingerprints were binary scored and dendrogram was constructed for ERIC and (GTG)5 fingerprint bands. ERIC PCR produced unique patterns consisting of 4 to 16 bands ranging between 0.15 kb to 1.5 kb. Two distinct polymorphic bands at 210 bp and 300 bp were found in all the serotypes. (GTG)5 PCR fingerprint consist of 3 to 15 bands ranging from 0.29 kb to 1.25 kb. All the serotypes tested revealed single polymorphic bands at 900 bp. ERIC and (GTG)5 dendrogram presented fingerprint for all the 19 isolates and serotypes were grouped into three major branches A, B and C which was further subdivided into small groups. The results obtained in the present study indicated ERIC-PCR and (GTG)5-PCR fingerprinting were not so efficient in discriminating Salmonella isolates.

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