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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 5 (may 2018) : 758-760,   Doi: 10.18805/ijar.B-3010

Rapid detection of infection due to Mycoplasma mycoides subsp. capri in experimental goats by PCR by assay  
 

K
K. Manimaran
V
V.P. Singh
1Central University Laboratory, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai-600 051, India.
Cite article:- Manimaran K., Singh V.P. (2017). Rapid detection of infection due to Mycoplasma mycoides subsp. capri in experimental goats by PCR by assay . Indian Journal of Animal Research. 52(5): 758-760. doi: 10.18805/ijar.B-3010.

ABSTRACT

Mycoplasma causes a number of important disease viz., CBPP, CCPP, CRD, Arthritis, Contagious agalactiae and Mastitis in livestock and poultry. The conventional method of isolation and identification of the causative agents of these diseases are generally employed in conjunction with the serology which have the limitation of cross reactivity with other mycoplasma and poor growth of the organisms. To overcome these problems, the PCR assay which is rapid, sensitive and specific was used in the present study to detect the mycoplasma infection due to M.Mycoides subsp.capri directly from the tissue materials viz., lungs, lymphnodes, spleen and liver from the experimentally infected  goats. All the tissue materials were  subjected  to PCR assay with cluster specific primers (MC 323 and MC 358), which yielded approximately 1.5 kbp long product. Further,. It was characterized by using mycoides group specific primers (MM 450 and MM 451) and Mycoplasma capri specific  primers (P 4 and P 6) which gave an amplicons of 574 bp and 195 bp products, respectively. The findings indicate  that the PCR assay  is very  simple and useful method for detecting the mycoplasma infection directly from the tissue materials in a very short span.

REFERENCES

  1. Amores, J., Corrales, J.C., Martin, AG., Sanchez,A., Contreras, A. and DelaFec (2010). Comparison of culture and PCR to detect. Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri in ear swabs taken from goats. Vet. Microbiol. Jan, 6 ; 140 : 105-108.
  2. Bashiruddin, J.B., Nicholas, R.A.J., Santini, F.G., Reddy,. R.A., Woodward, M.J., and Taylor, T.K. (1994 a). Use of PCR to detect mycoplasma DNA in cattle with CBPP. Vet. Res. 134: 240-241.
  3. Elnasri, M. (1967). Mycoplasma from contagious caprine pleuropnumonia. Ann.N.Y. Acad Sci. 143: 298-304.
  4. Hasso, S.A. and Al-Omaran, A.H., (1994) . Antibody response patterns in goats experimentally infected with Mycoplasma agalactiae ,Small Ruminants Res., 14: 79-81.
  5. Kumar, M.M., Sing, V.P. Srivastava, N.C., Singh, V.P. Sharma, B. Jaisundar and Kumar, A. (2001). Rapid and specific detection of Mycoplasma mycoides cluster and differentiation of mycoides group from capricolum group by polyumerase chain reaction. Indian J.Comp. Microbiolo.Imm. Infect. Dis., 22: 118-121.
  6. Laura Hernandez, Jose Lopez, Marcel st-Jacques, Lourdes ontivercos, Jorge Acosta and Katherine Handal (2006). Canadian Vet. J.n 47: 366-369.
  7. Litamoil, J.K, Lijodi, F.K. and Nandokha, E. (1989). Contagious caprine pleuropneumonia: Some observations in a field vaccination trial using inactivated mycoplasma strain F-38. Trop. Anim. Health Prod, 21 : 146-150.
  8. Rurangirwa, P.R., Masiga, N.N., and Muthomi, E.K., (1981). Immunity to contagious caprine pleuropneumonia caused by F-38 strain of mycoplasma. Vet. Rec. : 109: 310.
  9. Shirlaw, J.F., (1949). Studies on Contagious pleuropneumonia of the goat in India. Indian J. Vet. Sci., 19 : 181-213.
  10. Steel (1989). Cited form shjrlaw (1949).
  11. Singh, V.P. Srivastava, N.C., Tripatni, B.N., and Rana, R. (1997). Isolation of pathogenic Mycoplasma mycoides subsp.capri from pneumonic kid,. Indian J. Vet. Res., 6: 43-45
  12. Taylor, T.K. Bashiruddin, J.B., and Gould, A.R., (1992 b). Relationship between members of the mycoplasma mycoides cluster as shown by DNA probe and sequence analysis. Int. J. Syst., Bacteriol. 42: 593-601.
  13. Uppal, P.K., and Kumar, A.A., (1981). Studies on mycoplasma with special reference to diagnosis and development of caprine mycoplasma vaccine. Animal scientific report Division of Veterinary Bacteriology and mycology, IVRI, Izatnagar, U.P. India. 
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 5 (may 2018) : 758-760,   Doi: 10.18805/ijar.B-3010

    Rapid detection of infection due to Mycoplasma mycoides subsp. capri in experimental goats by PCR by assay  
     

    K
    K. Manimaran
    V
    V.P. Singh
    1Central University Laboratory, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai-600 051, India.
    Cite article:- Manimaran K., Singh V.P. (2017). Rapid detection of infection due to Mycoplasma mycoides subsp. capri in experimental goats by PCR by assay . Indian Journal of Animal Research. 52(5): 758-760. doi: 10.18805/ijar.B-3010.

    ABSTRACT

    Mycoplasma causes a number of important disease viz., CBPP, CCPP, CRD, Arthritis, Contagious agalactiae and Mastitis in livestock and poultry. The conventional method of isolation and identification of the causative agents of these diseases are generally employed in conjunction with the serology which have the limitation of cross reactivity with other mycoplasma and poor growth of the organisms. To overcome these problems, the PCR assay which is rapid, sensitive and specific was used in the present study to detect the mycoplasma infection due to M.Mycoides subsp.capri directly from the tissue materials viz., lungs, lymphnodes, spleen and liver from the experimentally infected  goats. All the tissue materials were  subjected  to PCR assay with cluster specific primers (MC 323 and MC 358), which yielded approximately 1.5 kbp long product. Further,. It was characterized by using mycoides group specific primers (MM 450 and MM 451) and Mycoplasma capri specific  primers (P 4 and P 6) which gave an amplicons of 574 bp and 195 bp products, respectively. The findings indicate  that the PCR assay  is very  simple and useful method for detecting the mycoplasma infection directly from the tissue materials in a very short span.

    REFERENCES

    1. Amores, J., Corrales, J.C., Martin, AG., Sanchez,A., Contreras, A. and DelaFec (2010). Comparison of culture and PCR to detect. Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri in ear swabs taken from goats. Vet. Microbiol. Jan, 6 ; 140 : 105-108.
    2. Bashiruddin, J.B., Nicholas, R.A.J., Santini, F.G., Reddy,. R.A., Woodward, M.J., and Taylor, T.K. (1994 a). Use of PCR to detect mycoplasma DNA in cattle with CBPP. Vet. Res. 134: 240-241.
    3. Elnasri, M. (1967). Mycoplasma from contagious caprine pleuropnumonia. Ann.N.Y. Acad Sci. 143: 298-304.
    4. Hasso, S.A. and Al-Omaran, A.H., (1994) . Antibody response patterns in goats experimentally infected with Mycoplasma agalactiae ,Small Ruminants Res., 14: 79-81.
    5. Kumar, M.M., Sing, V.P. Srivastava, N.C., Singh, V.P. Sharma, B. Jaisundar and Kumar, A. (2001). Rapid and specific detection of Mycoplasma mycoides cluster and differentiation of mycoides group from capricolum group by polyumerase chain reaction. Indian J.Comp. Microbiolo.Imm. Infect. Dis., 22: 118-121.
    6. Laura Hernandez, Jose Lopez, Marcel st-Jacques, Lourdes ontivercos, Jorge Acosta and Katherine Handal (2006). Canadian Vet. J.n 47: 366-369.
    7. Litamoil, J.K, Lijodi, F.K. and Nandokha, E. (1989). Contagious caprine pleuropneumonia: Some observations in a field vaccination trial using inactivated mycoplasma strain F-38. Trop. Anim. Health Prod, 21 : 146-150.
    8. Rurangirwa, P.R., Masiga, N.N., and Muthomi, E.K., (1981). Immunity to contagious caprine pleuropneumonia caused by F-38 strain of mycoplasma. Vet. Rec. : 109: 310.
    9. Shirlaw, J.F., (1949). Studies on Contagious pleuropneumonia of the goat in India. Indian J. Vet. Sci., 19 : 181-213.
    10. Steel (1989). Cited form shjrlaw (1949).
    11. Singh, V.P. Srivastava, N.C., Tripatni, B.N., and Rana, R. (1997). Isolation of pathogenic Mycoplasma mycoides subsp.capri from pneumonic kid,. Indian J. Vet. Res., 6: 43-45
    12. Taylor, T.K. Bashiruddin, J.B., and Gould, A.R., (1992 b). Relationship between members of the mycoplasma mycoides cluster as shown by DNA probe and sequence analysis. Int. J. Syst., Bacteriol. 42: 593-601.
    13. Uppal, P.K., and Kumar, A.A., (1981). Studies on mycoplasma with special reference to diagnosis and development of caprine mycoplasma vaccine. Animal scientific report Division of Veterinary Bacteriology and mycology, IVRI, Izatnagar, U.P. India. 
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