Microsatellite based diversity analysis of native pigs of North-Eastern India

DOI: 10.18805/ijar.7086    | Article Id: B-2994 | Page : 831-838
Citation :- Microsatellite based diversity analysis of native pigs of North-Eastern India .Indian Journal Of Animal Research.2016.(50):831-838

Nihar Ranjan Sahoo*1, Soumen Naskar, Santanu Banik and Prabhat Kumar Pankaj2

vet.nihar@gmail.com
Address :

ICAR-National Research Centre on Pig, Rani, Guwahati-781 131, Assam, India.

Submitted Date : 18-04-2015
Accepted Date : 18-08-2015

Abstract

The native pigs of North Eastern region have unique traits such as early sexual maturity and quality bristles compared with exotic/ crossbreds. An attempt was made to measure the genetic variation available within Niang Megha and Tenyi Vo pigs using 22 FAO-ISAG microsatellite markers. The analysis of microsatellite data revealed average number of observed alleles as 6.95±0.54 (Niang Megha) and 7.18±0.45 (Tenyi Vo) where as the effective average number of alleles were 3.55±0.33 and 3.81±0.41, respectively. The mean observed heterozygosities were found to be 0.61± 0.04 and 0.68± 0.05, whereas expected values were 0.67±0.03 and 0.69±0.03, for Niang Megha and Tenyi Vo pigs, respectively. Abundant genetic variations were displayed within the native pigs with the PIC values ranged between 0.41 (Sw2008) to 0.86. (S0218) for Niang Megha and between 0.45 (S0026) to 0.88 (S0005) in Tenyi Vo pigs. The mean F value was 0.15±0.05 in Niang Megha which is more than that found in Tevyi Vo.  There was no genetic bottleneck observed in the studied population. A total of 23 and 22 private alleles observed in Niang Megha and Tenyi Vo pigs. 
 

Keywords

Genetic variation Microsatellite Native pigs Niang Megha Population bottleneck Tenyi Vo.

References

  1. Anonymous (2008) Annual Report (2007-08). ICAR-National Research Centre on Pigs. Rani, Guwahati. Pp: 1-42
  2. Arora, R. and Bhatia, S. (2009). Evaluation of genetic effects of demographic bottleneck in Muzzafarnagri Sheep from I    ndia using microsatellite markers. Asian-Aust. J. Anim. Sci., 22:1-6.
  3. Behl, R., Kaul R., Sheoran N., Behl J., Tantia M.S. and Vijh R.K. (2002). Genetic identity of two Indian pig types using microsatellite markers. Anim. Genet., 33: 158–159.
  4. Behl, R., Sheoran N., Behl J. and Vijh R.K. (2006). Genetic analysis of Ankamali pigs of India using microsatellite markers and their comparison with other domesticated Indian pig types. J. Anim. Breed. Genet., 123: 131-5.
  5. Boettcher, P.J., Tixier-Boichard M., Toro M., Simianer H., Eding H., Gandini G., Joost S., Garcin D., Colli L., Ajmone-    Marsan P.and GLOBALDIV Consortium. (2010). Objectives, criteria and methods for using molecular genetic data in priority setting for conservation of animal genetic resources. Anim. Genet.,41: 64–77.
  6. Botstein, D., White R.L., Skolnick M. and Davis R.W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet.,32: 314–331.
  7. Fang, M., Hu X., Jiang T., Braunsweig M., Hu L., Du Z., Feng J., Zhang Q., Wu C. and Li N. (2005). The phylogeny of Chinese indigenous pig breeds inferred from microsatellite markers. Anim. Genet., 36: 7–13.
  8. Fredholm, M., Wintero A.K., Christensen K., Kristensen B., Nielsen P.B., Davies W. and Archibald A. (1993). Characterization of 24 porcine (AC)n(TG)n microsatellites: Genotyping of unrelated animals from four breeds and linkage studies. Mammalian Genome, 4:187-192.
  9. Goudet, J. (1995). FSTAT (vers. 2.9.3): a computer program to calculate F-statistics. J. Hered., 86:485-486.
  10. Karunakaran, M., Mandal M., Rajarajan K., Karmakar H.D., Bhatt B.P., Das J., Bora B., Baruah K.K. and Rajkhowa C. (2009). Early puberty in local Naga boar of India: Assessment through epididymal spermiogram and in vivo pregnancy. Anim. Reprod. Sci., 111: 112-119.
  11. Kumaresan, A., Bujarbaruah K.M., Karunakaran M., Das A. and Bardoloi R.K. (2008). Assessment of early sexual maturity in nondescript local pigs of north east India: testicular development, spermiogram and in vivo pregnancy. Livestock Sci., 116: 342-347.
  12. Laval, G., Iannuccelli N. and Legault C., et al. (2000). Genetic diversity of eleven European pig breeds. Genet. Sel. Evol., 32:187–203. 
  13. Lemus Flores, C., Ulloa-Arvizu R., Ramos-Kuri M., Estrada F. J. and Alonso R. A. (2001). Genetic analysis of Mexican hairless pig populations. J. Anim. Sci.,79: 3021–3026.
  14. Li, K.Y., Li K.T., Cheng C.C., Chen C.H., Hung C.Y., Ju Y.T. (2015). A genetic analysis of Taoyuan pig and its phylogenetic relationship to Eurasian pig breeds. Asian-Aust. J. Anim. Sci., 28: 457-466. 
  15. Li, X., Li K., Fan B., Gong Y., Zhao S., Peng Z. and Liu B. (2000). The genetic diversity of seven pig breeds in China, estimated by means of microsatellites. Asian-Aust. J. Anim. Sci., 13: 1193–1195.
  16. Luikart, G., Allendorf F.W., Cornuet J.M. and Sherwin W.B. (1998a). Distortion of allele frequency distributions provides a test for recent population bottleneck. J. Hered., 89: 238-247.
  17. Luikart G., Sherwin W.B., Steele B.M. and Allendorf F.W. (1998b). Usefulness of molecular markers for detecting population bottlenecks via monitoring genetic change. Mol. Ecolo., 7: 963-974. 
  18. Martinez, A.M., Delgado J.V., Rodero A. and Vega-Pla J.L. (2000). Genetic structure of Iberian pig breeds using microsatellites. Anim. Genet., 31: 295–301.
  19. Mohana, N.H., Debnath S., Mahapatra R.K., Nayak L.K., Baruah S., Das A., Banik S. and Tamuli M. K. (2014). Tensile properties of hair ûbres obtained from different breeds of pigs. Biosyst. Engineer., 119: 35-43
  20. National Bureau of Animal Genetic Resources. (2015). http://www.nbagr.res.in/registeredbreed.html
  21. Sahoo, N.R. Das A, Naskar S., Banik S. and Tamuli M.K. (2012) Niang Megha: The nature’s gift for food and fibre. National Research Centre on Pig (ICAR), Rani, Guwahati. Pp: 1-30
  22. Sambrook J.and Russell D.W.(2001). Molecular Cloning, a Laboratory Manual. 3rd Edition, Cold Spring Harbor Laboratory Press, NY, USA.
  23. 19th livestock Census. (2012). Department of Animal Husbandry, Dairy & Fisheries, M/ O Agriculture. Govt of India.
  24. Spencer, C.C., Neigel J.E. and Leberg P.L. (2000). Experimental evaluation of the usefulness of microsatellite DNA for detecting bottlenecks. Mol. Ecolo., 9: 1517-1528.
  25. Van-Zeveran, A., Peelman L., Van de Weghe A. and Bouquet Y. (1995). A genetic study of four Belgian pig populations by means of seven microsatelite loci. J. Anim. Breed. Genet., 112: 191–204. 
  26. Wang, X., Cao H. H., Geng S. M. and Li H. B. (2004). Genetic Diversity of 10 Indigenous Pig Breeds in China by Using Microsatellite Markers. Asian-Aust. J. Anim. Sci., 17: 1219-1222. 
  27. Wang, J.Y., Guo J. F., Zhang Q., Hu H. M., Lin H. C., Wang C., Zhang Y. and Wu Y. (2011). Genetic diversity of Chinese Indigenous pig breeds in Shandong Province using microsatellite markers. Asian-Aust. J. Anim. Sci., 24: 28-36. 
  28. Yeh, F.C., Yang R. and Boyle T. (1999). POPGENE Version 1.31, Microsoft Window Based Freeware for Population Genetic Analysis. University of Alberta and Centre for International Forestry Research. 

Global Footprints