Study on the in vitro fertilizing potential of mithun (Bos frontalis) semen

DOI: 10.18805/ijar.11323    | Article Id: B-2968 | Page : 909-914
Citation :- Study on the in vitro fertilizing potential of mithun (Bos frontalis) semen .Indian Journal Of Animal Research.2016.(50):909-914

P. Perumal*1, S.K. Srivastava2, K.K. Baruah3 and S.K. Ghosh2

perumalponraj@gmail.com
Address :

ICAR-National Research Centre on Mithun, Jharnapani, Nagaland – 797 106, India.

Submitted Date : 17-03-2015
Accepted Date : 10-06-2016

Abstract

Semen quality parameters (SQPs) such as motility, viability, total sperm abnormality, integrity of acrosome, plasma membrane and nucleus and vanguard distance travelled by sperm and biochemical profiles such as aspartate amino transaminase (AST), alanine amino transaminase (ALT) and lactate dehydrogenase (LDH) were studied in mithun at post thawed stage. Fifty ejaculates were collected through transrectal massage method from matured bulls and the ejaculates were divided into good and poor quality. The ejaculates were extended with Tris egg yolk citrate glycerol extender and stored in liquid nitrogen. PT seminal, biochemical profiles and zona binding per cent (BP) and binding index (BI) were studied with standard protocols. The results revealed good quality ejaculates has significantly (p<0.05) higher SQPs, BP & BI and has significantly (p<0.05) lower total sperm abnormality, AST, ALT and LDH than in the poor quality ejaculates. PT motility was positively (p<0.05) correlated with SQPs, vanguard distance, BI & BP and negatively correlated (p<0.05) with total sperm abnormality and biochemical profiles. In conclusion, the good quality sperm has higher functional sperm structures and higher intactness of sperm plasma and acrosomal membrane to motile faster in forward direction to reach the site of fertilization and successful fertilization.

Keywords

Mithun Post thaw semen Semen quality parameters.

References

  1. Barth, A.D. and Oko, R.J. (1989). Preparation of semen for morphological examination. In: Abnormal morphology of bovine spermatozoa, Iowa State University Press, Ames, IA. pp. 8-18.
  2. Bhoite, U.Y., Sutar, D.A. and Ulmek, B.R. (2005). Effect of season and period on semen characteristics of two and three breed Gir crosses. Indian J. Anim. Reprod. 26: 43-45.
  3. Dogan, I., Polat, U. and Nur, Z. (2009). Correlations between seminal plasma enzyme activities and semen parameters in seminal fluid of Arabian horses. Iran. J. Vet. Res. 10: 119-124.
  4. Fazeli, A.R., Steenweg, W., Bevers, M.M., de Loos, F.A.M., Van den Broek, J. and Colenbrander, B. (1993). Development of sperm zona pellucida binding assay for bull semen. Vet. Rec. 132: 14-16.
  5. Fiaz, M., Usmani, R.H., Abdullah, M. and Ahmad, T. (2010). Evaluation of semen quality of Holstein Friesian and Jersey bulls maintained under subtropical environment. Pak. Vet. J. 30: 75-78.
  6. Gebreselassie, G.A. (2009). Studies on physico-morphological characteristics and certain enzymes in freezable and non- freezable semen ejaculates of crossbred bulls. M.V.Sc. Thesis, Indian Veterinary Research Institute (Deemed University), Izatnagar, India.
  7. Javed, M.T., Khan, A. and Kauser, R. (2000). Effect of age and season on some semen parameters of Nili-Ravi buffalo bulls. Vet. Arch.70: 83-94.
  8. Jeyendran, R.S., Vander Ven, H. H., Parez-Pelaez, M., Crabo, B.G. and Zaneweld, L.J.D. (1984). Development of an assay to assess the functional integrity of the human membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 70: 219-228.
  9. Kumar, N., Verma, R.P., Singh, L.P., Varshney, V. P. and Dass, S.S. (2006). Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus × Bos taurus) bulls. Reprod. Nutri. Develop. 46: 663–675.
  10. Livestock Census, India (18th). (2007). Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture, Krishi Bhawan, New Delhi, India.
  11. López, M.L., Grez, P., Gribbel, I. and Bustos Obregón, E. (1989). Cytochemical and ultrastructural characteristics of the stallion epididymis (Equus cabalus). J. Submicroscopic Cyto. Patho. 21: 103-120.
  12. Loyi, T. (2008). Comparative studies on electrolytes and total seminal plasma protein between freezable and non- freezable crossbred bull semen. M.V.Sc. Thesis, Indian Veterinary Research Institute (Deemed University), Izatnagar, India.
  13. Matouseket, J., Riha, J., Sarsen, V., Veselky, H. and Londa, F. (1989). Penetration of cervical mucus and other body fluids by bull sperm in capillary tubes. Anim. Reprod. Sci. 18: 161- 166.
  14. Mishra, A.K. and Tyagi, S. (2006). Recent trends in the semen collection, evaluation and cryopreservation. In: National seminar on artificial insemination: Acceptability, impact, constraints and solution. pp. 95-105.
  15. Mohanty, D.N. (1999). Studies on reproductive soundness, freezability and fertility in CB bulls. Ph.D. Thesis, Indian Veterinary Research Institute (Deemed University), Izatnagar.
  16. Pace, M. M. and Graham, E. F. (1970). The release of glutamic oxaloacetic transaminase from bovine spermatozoa as a test method of assessing semen quality and fertility. Biol. Reprod. 3: 140-146.
  17. Perumal, P., Selvaraju, S., Selvakumar, S., Barik, A.K., Mohanty, D.N., Das, S., Das, R.K. and Mishra, P.C. (2011). Effect of pre-freeze addition of cysteine hydrochloride and reduced glutathione in semen of crossbred Jersey bulls on sperm parameters and conception rates. Reprod. Dom. Anim. 46: 636-641.
  18. Prasad, J.K. (1997). Studies on enzymatic changes and in vitro Fertility test in relation to cryopreservation of crossbred bull semen. M.V.Sc. Thesis, Indian Veterinary Research Institute (Deemed University), Izatnagar, India.
  19. Raval, R. J. and Dhami, A. J. (2010). Effect of additives on various spermatozoal attributes of fresh, Frozen-thawed and refrigerated semen. Indian J. Anim. Reprod. 31: 33-36.
  20. Sharma, M.L., Mohan, G. and Sahni, K.L. (1992). A study on acrosomal damage on cryopreservation of crossbred bull semen. Indian Vet. J. 69: 962-964.
  21. Singh, M., Ghosh, S. K., Prasad, J. K., Kumar, A., Ramteke, S.S. and Bhure, S.K. (2013). Heparin binding proteins of buffalo bulls seminal plasma and their relationship with semen freezability. Indian J. Anim. Sci. 83: 700–704.
  22. Tomar, N. S., Sharma, K. C. and Shukla, S. N. (1985). Semen production in relation to the age of HF x Hariana bulls. Indian Vet. J. 62: 499 – 501.
  23. Tyagi, S., Mathur, A. K. and Agarwal, S. C. (2000). Semen production performance of Frieswal bulls. Indian J. Anim. Sci. 70: 1032-1034.
  24. Watson, P.F. (1975). Use of Giemsa stain to detect change in acrosome of frozen ram spermatozoa. Vet. Rec. 97: 12-15.
     

Global Footprints