Efficacy of diatomacious earth in amelioration of aflatoxin induced toxicity in broiler chicken

DOI: 10.18805/ijar.10974    | Article Id: B-2902 | Page : 529-536
Citation :- Efficacy of diatomacious earth in amelioration of aflatoxininduced toxicity in broiler chicken .Indian Journal Of Animal Research.2016.(50):529-536

A.W. Lakkawar*, M.L. Sathyanarayana1, H.D. Narayanaswamy, Sugunarao, S. Yathiraj,  Isloor S.K., N.B. Shridhar2 and N. Krishnaveni

dralakkawar@yahoo.com
Address :

Department of Pathology, Veterinary College, Bengaluru- 560 024, Karnataka India.

Submitted Date : 5-11-2014
Accepted Date : 29-04-2015

Abstract

The ability of Diatomacious earth (DAE) in reducing the toxic effects of aflatoxin (AF) in broiler diet was evaluated. DAE was supplemented @ 400 and 800 mg kg-1 of feed along with 0.5 and 1 ppm of AF kg-1 of feed. Healthy unsexed day old broiler chicks (n=360) were assigned to 9 groups comprising of control and treatment groups. Feeding of AF resulted in significantly lower feed intake as well as body weight gain and increase in feed conversion ratio in comparison to the control groups. Supplementation of DAE in aflatoxin mixed diet significantly reduced the deleterious effects of AF on growth parameters. Feeding of AF alone caused significant decrease in serum levels of total proteins, albumin, triglycerides and cholesterol. However, significant increase was found in serum levels of AST, ALT, ALP, BUN and creatinine in AF fed broilers. Supplementation of DAE to the AF mixed feed ameliorated the adverse effect of AF on the serum biochemical values by causing increase in serum total proteins, albumin, triglycerides and cholesterol levels. In addition, a significant increase was recorded in AST, ALT, ALP, BUN and creatinine levels in DAE supplemented broilers. The study concluded that diatomaceous earth is an effective adsorbent to decrease the negative effects of aflatoxin in broiler chicken.

Keywords

Aflatoxin Amelioration Broilers Diatomaceous earth Growth performance Serum biochemistry.

References

  1. Abdel-Wahhab, M.A., Nada, S.A and Amra, H. A. (1999).Effect of aluminosilicates and bentonite on aflatoxin-induced developmental toxicity in rat. J. Appl. Toxicol. 19:199–204.
  2. Alves, (2006) Action of diatomaceous earth against adults of the lesser meal worm Aliphitobius. Diaperinus (Panzer, 1797) (Coleoptera:Tenebrionidae). Arq. Inst. Bio., São Paulo.,73: 115-118. 
  3. Bailey C.A., Latimer G.W., Barr A.C., Wigle W.L., Haq A.U., Balthrop J.E. and Kubena L.F., (2006) Efficacy of montmorillonite clay (NovaSil PLUS) for protecting full-term broilers from aflatoxicosis. J. Appl. Poult. Res. 15: 198–206.
  4. Bennett D.C., Yee A.,Rhee Y.J., and Cheng K.M., (2011) Effect of diatomaceous earth on parasite load, egg production, and egg quality of free-range organic laying hens. Poult. Sci., 90: 1416–1426 
  5. Bintvihok, A., Thiengnin S., Doi K. and Kumagai S. (2002) Residues of aflatoxins in the liver, muscle and eggs of domestic fowls. J. Vet. Med. Sci., 64:1037–1039.
  6. Celik, K., Denli, M., Erturk, M., Ozturkcan, O. and Doran, F. (2001).Evaluation of dry yeast Saccharomyces Cerevisiae in the feed to reduce aflatoxin B1 residues and toxicity to Japonica Quails (Coturnixcoturnix Japonica). J. Appl. Anim. Res. 20: 245-250
  7. Dafalla R., Yagi A., Adam S.E., (1987) Experimental aflatoxicosis in hybro-type chicks ; sequential changes in growth and serum constituents and histopathological changes. Vet. Hum. Toxicol., 29: 222-226.
  8. Denbow D (2000). Gastrointestinal anatomy and physiology. In: Whittow GC, editor. Sturkie’s Avian Physiology 5th ed. Academic Press, San Diego
  9. Denli, M. and Okan F. (2006) Efficacy of different adsorbents in reducing the toxic effects of aflatoxin B1 in broiler diets. South African Journal of Animal Science, 36: 222-228
  10. Denli, M., Blandon J.C., Guynot M.E., Salado S.and Perez J.F. (2009) Effects of dietary AflaDetox on performance, serum biochemistry histopathological changes, and aflatoxin residues in broilers exposed to aflatoxin B1. Poult. Sci, 88: 1444-1451.
  11. Dhanapal, S.K, Rao S., Govindaraju P.K.P., Hukker R. and Mathesh K. (2014) Ameliorative Efficacy of Citrus Fruit Oil in Aflatoxicosis in Broilers: A Growth and Biochemical Study. Turk. J. Vet. Anim. Sci. 38: 207-211
  12. Duncan, D. B. (1955) Multiple Range and Multiple F tests. Biometrics, 1: 1-42.
  13. Gao S.W., Clanton C.J., Schmidt D.R.,. Janni K A., Jacobson L.D. and Weisberg S. (2002) Odor, total reduced sulfur and ammonia emissions from livestock and poultry buildings and manure storage units. ASAE, Applied Engineering in Agri.,
  14. Hamilton P.B. (1984) Determination safe levels of mycotoxins, J. Food Protect., 47: 575.
  15. Huwig A., Freimund S., Kappeli O., Dutler H. (2001) Mycotoxin detoxication of animal feed by different adsorbents, Toxicol. Lett., 122: 179-188.
  16. Jindal N., Mahipal S.K. and Mahajan N.K. (1994) Toxicity of aflatoxin B1 in broiler chickens and its reduction by activated charcoal. Res. Vet. Sci., 56: 37-40.
  17. Kamigasa, S. and Kato H. (2000) Recent conditions and prospects of diatomite resources, Energy Resources, 21: 166-172.
  18. Ledoux D.R., Rottinghaus G.E., Bermudez A.J. and Alonso-Debolt M. (1999) Efficacy of hydrated sodium calcium aluminosilicate to ameliorate the toxic effects of aflatoxin in broiler chicks. Poult. Sci.,78: 204-210
  19. Leeson S., Diaz G.and Summers J.D. (1995) Aflatoxin. In: Poultry Metabolic Disorders. Med 17:314-324
  20. Miller J.D. (1995) Fungi and mycotoxins in grain: implications for stored product research. J. Stored Prod. Res., 31:1-16.
  21. Modirsanei M, Mansoori B., Khosravi A. R., Kiaei M. M., Khazraeinia P., Farkhoy M., Masoumi Z. (2008) Effect of diatomaceous earth on the performance and blood variables of broiler chicks during experimental aflatoxicosi., J. Sci. Food and Agriculture, 88: 626-632
  22. Natour, R.M. and Yousef, S.M. (1998). Adsorption efficiency of diatomaceous earth for mycotoxin. Arab Gulf Journal of Scientific Research 16: 113–127.
  23. Parlat S.S., Yildiz A.O., Oguz H. (1999) Effect of clinoptilolite on performance of japanese quail (conturnix japonica) during experimental aflatoxicosis. Br. Poult. Sci., 40: 495-500.
  24. Ramos A.J. and Hernandez E. (1997) Prevention of aflatoxicosis in farm animals by means of hydrated sodium calcium aluminosilicate addition to feedstuff: A review. Anim. Feed Sci. Technol., 65: 197-206.
  25. Rosa C.A.R., Miazo R., Oli C., Salvano M., Chiacchiera S.M., Ferrero S., Saenz Carvalho E.C.Q. and Dalcero A. (2001) Evaluation of the dietary effect of bentonite from the south of Argentina in ameliorating the toxic effects of aflatoxin in broilers. Poult, Sci.,80: 139-144
  26. Scheideler S.E. (1993) Effects of various types of aluminosilicates and aflatoxin B1 on aflatoxintoxicity,chick performance and mineral status. Poult. Sci., 72: 282-288.
  27. Shankaran, R., Raj H. G. and Venkatasubramanian T. A. (1970) Effect of aflatoxinon carbohydrate metabolism in chick liver. Enzymlogia, 39: 371-378
  28. Shi, Y.H., Xu Z.R, Feng Z.R. and Wang C. Z. (2006) Efficacy of modified montomorillonitenanocomposite to reduce the toxicity of aflatoxin in broiler chickens. Anim. Feed Sci. Tech., 129: 138
  29. Shotwell O.L., Hesseltine C.W., Stubblefeild R.D. and Sorenson W.G. (1966) Production of aflatoxin on rice. Appl. Microbiol.,52: 425-428.
  30. Soleimani R, OP Faradonbeh and H Bagheri (2011). Mycotoxin detoxification of commercial broiler feed by a mycotoxin binder. Res. Opin. Anim . Vet. Sci., 1: 778-780.
  31. Swamy, H.V.L.N. and Devegowda G. (1998). Ability of Mycosorb to counteract aflatoxicosis in commercial broilers. Indian J. Poult. Sci., 33: 279-278
  32. Umar, S., A. Arshad, B. Ahmad and M. Arshad, 2012.Clinico biochemical and haematological changes in broilers induced by concurrent exposure to aflatoxin B1 and ochratoxin A. Journal of Public Health and Biological Sciences, 1: 79–85.
  33. Valchev, D., Kanakov, T.S. Hristov L., Binev R., Grozeva N., Nikolov Y. (2014). Effects of
  34. Verma, R.J. (2004) Aflatoxin cause DNA damage. Int. J Human Genetics, 4: 231-236.
  35. Vieira SL (2003). Nutritional implication of mould development in feed stuff and alternatives to reduce the mycotoxins problem in poultry feed. World Poult Sci J. 59: 111
  36. Wajima T., Haga M., Kuzawa K., Ishimoto H., Tamada O., Ito K., Nishiyama T., Downs R.T. and Rakovan J.F. (2006) Zeolite synthesis from paper sludge ash at low temperature (90 degrees C) with addition of diatomite. J. Hazard. Mat.,132: 244-252.
  37. Whitlow L.W. (2006) Evaluation of mycotoxin binders, 4th Mid-Atlantic Nutrition Conference (Zimmerman, N.G. ed.), Proceedings, 132-143

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