Probiotic activity of Bifidobacteria from infant faeces
Citation :- Probiotic activity of Bifidobacteria from infant faeces .Indian Journal Of Animal Research.2016.(50):63-68
In the present study four isolates belonging to threebifidobacterial species with probiotic properties were isolated and identified from the faeces of breast fed infants and incorporated in the preparation of a nutraceutical whey based malt food. Identification of isolates to the genus Bifidobacteria was based on phenotypic characteristics like the unique pleomorphic morphology and carbohydrate fermentation profile. Molecular tools like 16S rRNA targeted genus and species specific mPCR were used for confirmation. The four isolates (of 3 species) identified wereB. longum(IB10 and IB12)B. breve (IB39)and B.bifidum (IB42). The nucleotide sequences of the identified species were submitted to the GenBank for acquisition of accession number. The identified species were screened for probiotic properties like bile tolerance, antimicrobial activity and tolerance to acidity. All the four isolates showed more than 80 per cent viability in the presence of 0.4, 0.8 and 1 per cent of bile salt. IsolateB.longum (IB10)showed maximum antimicrobial activity against Bacillus cereus (zi3.03 ± 0.019 mm), Staphylococcus aureus (zi3.00 ± 0.031mm), Escherichia coli(zi2.98 ± 0.043 mm) and Pseudomonas aeruginosa (zi3.03 ± 0.051 mm) in the cell free supernatant . All the four bifidobacterial isolatesshowed more than 80 per centviability atpH 3.B.longum (IB10)was a better probiotic when compared to other isolates as it had maximum antimicrobial activity and tolerance to bile and acidity.
Isolation of bifidobacteria by mPCR
- Ahmad Cheikyoussef, NataschaPogori, Haqin Chen, Fengwei Tian, Wei Chen, Jian Tang and Hao Zhang, (2009). Antimicrobial activity and partial characterization of bacteriocin like inhibitory substances (BLIS) produced by Bifidobacteriuminfantis BCRC 14602. Food Control, 20: 553-559.
- Anand, S.K., Srinivasan R.A. and Rao, L.K. (1985). Antimicrobial activity associated with Bifidobacterium bifidum-1. Cult. Dairy Prod.J., 11: 6 – 7.
- Arunachalam, K., Gill H.S. and Chandra, R.K. (2000). Enhancement of natural immune function by dietary consumption of Bifidobacteriumlactis (HNO19). Eur .J. of Clin. Nutr., 54: 263-7
- Dong, X., Cheng G. and Jian, W. (2000). Simultaneous identification of five Bifidobacterium species isolated from human beings using multiple PCR primers. Syst.Appl.Microbiol.,23: 386-390
- FAO/WHO Working group Report, 2002. Guidelines for the evaluation of probiotics in food. London, Ontario, Canada.
- Garriga, M., Pascual, M. Montford J.M. and Hugas, M. (1998). Selection of Lactobacilli for chicken probiotic adjuncts. J.of Appl. Microbiol. 84: 125-132
- Gerald Tannock, W., (1999). Identification of Lactobacilli and Bifidobacteria.Current IssuesMolec. Biol., 1: 53-64.
- Gilliland, S.E., Staley T.E. and Bush, L.J. (1984). Importance of bile tolerance of L.acidophilus used as dietary adjunct. J. of Dairy Sci., 67: 3045 - 3051
- Giovanna Felis, E. and Franco Dellaglio, (2007). Taxonomy of Lactobacilli and Bifidobacteria.Curr. Issues of Intest. Microbiol. , 8: 44–61.
- Indian Council of Medical Research –Department Biotechnology, (2011).Guidelines for Evaluation of Probiotics in Food, Published by Indian Council of Medical Research, New Delhi.
- Jaya Prasad, Gill, Harsharanjit Smart John and Gopal, Pramod K. (1999). Selection and characterization of Lactobacillus and Bifidobacterium strains for use as probiotics.Int.Dairy J., 8: 993 – 1002.
- Korshunov, V.M., Gudieva, Z.A. Efimov, B.A. Pikina, A.P. Smeianov, V.V. Reid, G. Korshunova, C.V. Tiutiunnik V.L. and Stepin, I.I. (1999). The vaginal Bifidobacterium flora in women of reproductive age, Zh, Mikrabiol Epidemiol. Immunobiol.,4: 74-78.
- Matsuki, T., K. Watanabe, R. Tanaka, (2003). Genus and species – specific PCR premiers for the detection and identification of Bifidobacteria.Curr.Issues Intest.Microbiol.,4: 61 – 69.
- Metchnikoff, E., (1907). Lactic acid as inhibiting intestinal putrefaction. In: 1. The prolongation of life: Optimistic studies. W. Heinemann, London: 161-183.
- Michael, A. Tunner, J. William Coleman, L. Christine Everett, J. Steven Robles, R. Michael Dilivorth, M. Mary Yang and C. Douglas Youvan, (2000). MultispectralBacterial Identification.Biotechnol.et alaia,6: 1–9
- Mullie, C., M.F. Odou, E. Singir, M.B. Romond and D. Izard, (2003). Multiplex PCR using 16S rRNA gene targeted primers for the identification of Bifidobacteria from human origin, FEMS Microbiol. Lett.,222: 129–136.
- Pikina.A.P., Smeianov, V.V. Efimov, B.A. Bainov, N.A. Brooks, I. Reeves G. and Korshunov, V.M. (1999). The primary screening of bifidobacteria and lactobacilli strains to develop effective probiotic preparations based on them. Zh. Mikrabiol.epidemiol immunobiol.,6 (34)
- Ruben,G. Kok, Wal, Anthony De, schut Frist and Weenik, Gerald (1996). Specific detection and analysis of a probiotic Bifidobacterium strain in infant faeces. Appl.Environ.Microbiol., 62: 3668-3672.
- Scardovi, V., (1986). The genus Bifidobacterium.In: Holf J. G, Editor, Bergey’s Manual of systematic Bacteriology Vol.2; Boltimore: Williams and Wilkins Co; p.1414.
- Suresh Subramonian, B., (2000). Studies on preparation of dietetic milk powder with added bifidogenic properties. Ph.D. Thesis submitted to Tamil Nadu
- Tamime, A. Y., Valerie M.E. Marshall and Richard Robinson, K. (1995). Microbiological and technological aspects of milks fermented by bifidobacteria. J. of Dairy Res., 62: 151–187.
- Vlkova.E., Neviral, J. Jencikova, B. Kopeeny, J. Godefrooij, J. Trojanava I. and Rada, V. (2005). Detection of infant faecal Bifidobacteria by enzymatic methods.J. of Microbiol. Methods,60: 365–373.
- Weisburg, W.G., S.M. Barns, Pelletier D.A. and Lane, D.J. (1991). 16S Ribosomal DNA amplification for Phylogenetic Study. J.Bacteriol., 173: 697.
- Zinedine A and Faid, M. (2007). Isolation and characterization of strains of Bifidobacteria with probiotic properties.World J. Dairy and Food Sci. 2: 28 – 34.