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volume 49 issue 2 (april 2015) : 196-198,   Doi: 10.5958/0976-0555.2015.00092.8

Molecular characterization of Cytochrome B gene in indigenous pig

J
Jnyanashree Saikia*
T
T. C. Roy
S
S. Naskar
B
B. Das
A
A.M. Ferdoci
1Department of Animal Genetics and Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati -781022, India.
Cite article:- Saikia* Jnyanashree, Roy C. T., Naskar S., Das B., Ferdoci A.M. (2025). Molecular characterization of Cytochrome B gene in indigenous pig. Indian Journal of Animal Research. 49(2): 196-198. doi: 10.5958/0976-0555.2015.00092.8.

ABSTRACT

In pigs, genetic variability at the Cytochrome B (Cyt B) gene of mitochondrial DNA (mt DNA) has been widely used as a tool to determine genetic relationships between different breeds and population. The present study was carried out with the objectives to find out SNPs in Cyt B gene and decipher evolutionary relationship among the indigenous pigs based on it. Blood samples of 104 indigenous pigs viz.Ghungroo, Meghalaya local and Nagaland local, were collected and mt DNA was isolated. PCR-SSCP was carried out revealing monomorphic pattern comprising four similar bands. Representative samples of 3 different breeds were subsequently sequenced. Sequence analyses revealed presence of 4 SNPs at position 47(C), 49(A), 52(T) and 56(A) which corresponds to 15036, 15038, 15041 and 15045 nucleotide positions of mitochondrial genome, respectively. This particular combination of SNPs of CytB gene denoted Asiatic origin of indigenous pigs-further pointing to A1 haplotype.

REFERENCES

  1. Brown W M, George M J and Wilson A C. (1979). Rapid evolution of mitochondrial DNA. Proceedings of the National Academy of Sciences of USA 76: 67-71.
  2. Clop A, Noguera J L, Fernandez A, Capote J, Ramon M M, Kelly L, Kijas J M H, Andersion L and Saznchez A. (2004). Estimating the frequency of Asian cytochrome B haplotypes instandard European and local Spanish pig breeds. Genet. Sel. Evol., 36: 97-104.
  3. Fang M and Andersson L. (2006). Mitochndrial diversity in European and Chinese pigs is consistent with population expansions that occurred prior to domestication. Proceedings of the Royal Society, 273:1803-1810.
  4. Giuffra E, Kijas J M H, Amarger V, Carlborg O, Jeon J T and Andersson L. (2000). The origin of the domestic pig: independence domestication and subsequent introgression. Genet., 154:1785-1791.
  5. Kijas J M and Andersson L. (2001). A phylogenetic study of the origin of domestic pig estimated from the near complete mtDNA genome. J. Mol. Evol., 52(3): 302-308.
  6. Kim K I, Lee J H, Li K, Zhang Y P, Lee SS, Gongora J and Moran C. (2002). Phylogenetic relationships of Asian and European pig breeds determined by mitochondrial DNA D-loop sequence polymorphism. Anim. Genet., 33:19-25.
  7. Sambrook J and Russel D W. (2001). Molecular Cloning: A laboratory manual, 3rd Edn., Cold Spring Harbour Laboratory Press, New York.
  8. Toro M A, Rodriganez J, Silio L and Rodriguez M C. (2000). Genealogical analysis of a closed herd of black hairless Iberian pigs. Cons. Biol., 14: 1843-1851.
  9. Watanabe T, Hayashi Y, Ogasawara N and Tomoita T. (1985). Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease patterns. Biochemical Genetics., 23: 105-113.
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Indian Journal of Animal Research
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    volume 49 issue 2 (april 2015) : 196-198,   Doi: 10.5958/0976-0555.2015.00092.8

    Molecular characterization of Cytochrome B gene in indigenous pig

    J
    Jnyanashree Saikia*
    T
    T. C. Roy
    S
    S. Naskar
    B
    B. Das
    A
    A.M. Ferdoci
    1Department of Animal Genetics and Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati -781022, India.
    Cite article:- Saikia* Jnyanashree, Roy C. T., Naskar S., Das B., Ferdoci A.M. (2025). Molecular characterization of Cytochrome B gene in indigenous pig. Indian Journal of Animal Research. 49(2): 196-198. doi: 10.5958/0976-0555.2015.00092.8.

    ABSTRACT

    In pigs, genetic variability at the Cytochrome B (Cyt B) gene of mitochondrial DNA (mt DNA) has been widely used as a tool to determine genetic relationships between different breeds and population. The present study was carried out with the objectives to find out SNPs in Cyt B gene and decipher evolutionary relationship among the indigenous pigs based on it. Blood samples of 104 indigenous pigs viz.Ghungroo, Meghalaya local and Nagaland local, were collected and mt DNA was isolated. PCR-SSCP was carried out revealing monomorphic pattern comprising four similar bands. Representative samples of 3 different breeds were subsequently sequenced. Sequence analyses revealed presence of 4 SNPs at position 47(C), 49(A), 52(T) and 56(A) which corresponds to 15036, 15038, 15041 and 15045 nucleotide positions of mitochondrial genome, respectively. This particular combination of SNPs of CytB gene denoted Asiatic origin of indigenous pigs-further pointing to A1 haplotype.

    REFERENCES

    1. Brown W M, George M J and Wilson A C. (1979). Rapid evolution of mitochondrial DNA. Proceedings of the National Academy of Sciences of USA 76: 67-71.
    2. Clop A, Noguera J L, Fernandez A, Capote J, Ramon M M, Kelly L, Kijas J M H, Andersion L and Saznchez A. (2004). Estimating the frequency of Asian cytochrome B haplotypes instandard European and local Spanish pig breeds. Genet. Sel. Evol., 36: 97-104.
    3. Fang M and Andersson L. (2006). Mitochndrial diversity in European and Chinese pigs is consistent with population expansions that occurred prior to domestication. Proceedings of the Royal Society, 273:1803-1810.
    4. Giuffra E, Kijas J M H, Amarger V, Carlborg O, Jeon J T and Andersson L. (2000). The origin of the domestic pig: independence domestication and subsequent introgression. Genet., 154:1785-1791.
    5. Kijas J M and Andersson L. (2001). A phylogenetic study of the origin of domestic pig estimated from the near complete mtDNA genome. J. Mol. Evol., 52(3): 302-308.
    6. Kim K I, Lee J H, Li K, Zhang Y P, Lee SS, Gongora J and Moran C. (2002). Phylogenetic relationships of Asian and European pig breeds determined by mitochondrial DNA D-loop sequence polymorphism. Anim. Genet., 33:19-25.
    7. Sambrook J and Russel D W. (2001). Molecular Cloning: A laboratory manual, 3rd Edn., Cold Spring Harbour Laboratory Press, New York.
    8. Toro M A, Rodriganez J, Silio L and Rodriguez M C. (2000). Genealogical analysis of a closed herd of black hairless Iberian pigs. Cons. Biol., 14: 1843-1851.
    9. Watanabe T, Hayashi Y, Ogasawara N and Tomoita T. (1985). Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease patterns. Biochemical Genetics., 23: 105-113.
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