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volume 49 issue 4 (august 2015) : 423-428,   Doi: 10.5958/0976-0555.2015.00136.3

Molecular genetic characterization of Berari breed of goat using microsatellite markers

K
Kranti Kharkar
S
S.V. Kuralkar
P
Prajakta Kuralkar
1Department of Animal Genetics and Breeding, Post Graduate Institute of Veterinary and Animal Sciences, Akola- 444 104 India.
Cite article:- Kharkar Kranti, Kuralkar S.V., Kuralkar Prajakta (2025). Molecular genetic characterization of Berari breed of goat using microsatellite markers. Indian Journal of Animal Research. 49(4): 423-428. doi: 10.5958/0976-0555.2015.00136.3.

ABSTRACT

Berari is 23rd recognized goat breed of India with accession number INDIA_GOAT_1100_BERARI_06023. A battery of 25 selected micro-satellite markers was used to study the genetic variability in Berari goat breed. The observed number of alleles ranged from 9 (ILSTS059, RM004) to 25 (ILSTS005, ILSTS029) with an average value of 15.560±0.947. The effective number of alleles ranged from 6.036 (RM088) to 16.736 (ILSTS029) with the overall mean value of 10.214±0.602. The average expected gene diversity within the population ranged from 0.834 (RM004) to 0.940 (ILSTS029) with an overall mean of 0.895±0.006. All of the studied loci showed the polymorphic information content value greater than 0.5 with an average value of 0.886±0.007, these high values of PIC indicated higher polymorphism in the breed. The values Shannon’s information index were found be in the range from 1.946 (LSTS059) to 2.981 (ILSTS029) with an average 2.456±0.058. Sixteen out of total 25 studied loci showed significant heterozygote deficiency (positive Fis value). The positive values of the Fis ranged from 0.008 (ILSTS034) to 0.599 (OMHC1) with an overall mean of 0.125. Bottleneck was examined assuming all three mutations models and was found to be absent. The high level of genetic variability, however, suggested the scope for further genetic improvement of Berari goats.

REFERENCES

  1. Aggarwal, R. A. K.; Dixit, S. P.; Verma, N. K.; Ahlawat, S. P. S.; Kumar, Y.; Kumar, S.; Chander, R. and Singh, K. ( 2007). Population genetics analysis of Mehsana goat based on microsatellite markers. Current Science 92 (8) : 1133–1137.
  2. Bassam, B. J.; Coetano-Anolles, G. and Gresshoff, P. M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry 196: 80-83.
  3. Botstein, D.; White, R. L.; Skolnick, M. and Davis, R. W. (1980). Construction of a genetic linkage map in man using restriction fregment length polymorphism. American J. Human Genetics 32 : 324-331.
  4. Cornuet, J. M. and Luikart, G. (1996). Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics 144: 2001–2014.
  5. Dixit, S. P.; Verma, N. K.; Ahlawat, S. P. S.; Aggarwal, R. A. K.; Kumar, S.; Chander, R. and Singh, K. P. (2008). Molecular genetic characterization of Kutchi breed of goat. Current Science 95 (7): 946-952.
  6. Dixit, S. P.; Aggarwal, R. A. K.; Verma, N. K.; Vyas, M. K.; Rana, J.; Sharma, A. and Chander, R. (2011). Genetic variability and bottleneck analyses of Kanniadu goat breed based on microsatellite markers. Indian J. Anim. Sci. 81 (1): 40-43.
  7. Dixit, S. P.; Aggarwal, R. A. K.; Dandi P. S.; Verma, N. K.; Vyas, M. K.; Rana, J.; Sharma, A.; Kharadi, V. B.; Sabapara, G. P. and Deshpande, S. B. (2013). Phenotypic characteristics, management, performance and genetic variability in Surati breed of goat. Indian J. Anim. Sci. 83 (4) : 423-427.
  8. Di Rienzo, A.; Peterson, A. C.; Garca, J. C.; Valdes, A. M. and Slatkin, M. (1994). Mutational proceses of simple sequence repeats in human populations. Proceedings of National Academy of Sciences, USA 91: 3168–70.
  9. Kumar, D.; Dixit ,S. P.; Verma, N. K.; Singh, D. K.; Pande, A.; Kumar, S.; Chander, R. and Singh, L. B. (2009). Genetic diversity analysis of the Gohilwari breed of Indian goat (Capra hircus) using microsatellite markers. American J. Anim. Vet. Sci. 4 (3) : 49-57.
  10. Kimura, M. and Crow, J. W. (1964). The number of alleles that can be maintained in a finite population. Genetics 49: 725–738.
  11. Levene, H. (1949). On a matching problem arising in genetics. The Annals of Mathematic Statistics 20: 91–94.
  12. Mishra, P.; Verma, N. K.; Aggarwal, R. A. K. and Dixit, S. P. (2010). Breed characteristics and genetic variability in Changthangi goats. Indian J. Anim. Sci. 80 (12) : 1203–1209.
  13. Mishra, P.; Ali, S. A. and Verma, N. K. (2012). Phenotypic, biometric and genetic characterization of Bundelkhandi goats. Indian J. Anim. Sci. 82 (11) : 1442–1445.
  14. Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proceedings of National Academy of Sciences USA 70: 3321– 3323.
  15. Ohta, T. and Kimura, M. (1973). A model of mutation appropriate to estimate the number of electrophoretically detectable alleles in a finite population. Genetic Research Cambridge 22: 201–204
  16. Sambrook, J. and Russell, D. W. (2001). Molecular Cloning : A Laboratory Manual. 3rd edn. Cold Spring Harbour Cold Spring Laboratory Press NY.
  17. Shannon, C. E. and Weaver, W. (1949). The Mathematical Theory of Communication. University of Illinois Press, IL.
  18. Sharma, R.; Kumar, D.; Pandey, A. K.; Dixit, S. P. and Ahlawat, S. P. S. (2008a) . Genetic variability and bottleneck analysis of Barbari goat population using microsatellite markers. Indian J. Anim. Sci. 78 (4) : 391-396.
  19. Sharma, R.; Kumar, D.; Pandey, A. K.; Dixit, S. P. and Ahlawat, S. P. S. (2008b). Genetic variability in Beetal goat breed assessed by microsatellite markers. Indian J. Anim. Sci. 78 (5):505:509.
  20. Takezaki, N. and Nei, M. (1996) Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genetics 144: 389-399.
  21. Verma, N. K.; Dixit, S. P.; Aggarwal, R. A. K.; Chander, R.; Kumar, S. and Ahlawat, S. P. S. (2007). Molecular genetic analysis of Sirohi breed of goat. Korean J. Genet. 29: 129-136.
  22. Vijh, R K.; Tantia, M. S.; Bhel, R. and Mishra, B. (2010). Genetic architecture of Black Bengal and Chegu goat. Indian J. Anim. Sci. 80 (12): 1134-1137.
  23. Yeh, F. C.; Boyle, T.; Rongcai, Y.; Ye, Z. and Xian, J. M. (1999). POPGENE version 3.1(http://www.ualberta.ca/-fyeh/fyeh.)
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    volume 49 issue 4 (august 2015) : 423-428,   Doi: 10.5958/0976-0555.2015.00136.3

    Molecular genetic characterization of Berari breed of goat using microsatellite markers

    K
    Kranti Kharkar
    S
    S.V. Kuralkar
    P
    Prajakta Kuralkar
    1Department of Animal Genetics and Breeding, Post Graduate Institute of Veterinary and Animal Sciences, Akola- 444 104 India.
    Cite article:- Kharkar Kranti, Kuralkar S.V., Kuralkar Prajakta (2025). Molecular genetic characterization of Berari breed of goat using microsatellite markers. Indian Journal of Animal Research. 49(4): 423-428. doi: 10.5958/0976-0555.2015.00136.3.

    ABSTRACT

    Berari is 23rd recognized goat breed of India with accession number INDIA_GOAT_1100_BERARI_06023. A battery of 25 selected micro-satellite markers was used to study the genetic variability in Berari goat breed. The observed number of alleles ranged from 9 (ILSTS059, RM004) to 25 (ILSTS005, ILSTS029) with an average value of 15.560±0.947. The effective number of alleles ranged from 6.036 (RM088) to 16.736 (ILSTS029) with the overall mean value of 10.214±0.602. The average expected gene diversity within the population ranged from 0.834 (RM004) to 0.940 (ILSTS029) with an overall mean of 0.895±0.006. All of the studied loci showed the polymorphic information content value greater than 0.5 with an average value of 0.886±0.007, these high values of PIC indicated higher polymorphism in the breed. The values Shannon’s information index were found be in the range from 1.946 (LSTS059) to 2.981 (ILSTS029) with an average 2.456±0.058. Sixteen out of total 25 studied loci showed significant heterozygote deficiency (positive Fis value). The positive values of the Fis ranged from 0.008 (ILSTS034) to 0.599 (OMHC1) with an overall mean of 0.125. Bottleneck was examined assuming all three mutations models and was found to be absent. The high level of genetic variability, however, suggested the scope for further genetic improvement of Berari goats.

    REFERENCES

    1. Aggarwal, R. A. K.; Dixit, S. P.; Verma, N. K.; Ahlawat, S. P. S.; Kumar, Y.; Kumar, S.; Chander, R. and Singh, K. ( 2007). Population genetics analysis of Mehsana goat based on microsatellite markers. Current Science 92 (8) : 1133–1137.
    2. Bassam, B. J.; Coetano-Anolles, G. and Gresshoff, P. M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry 196: 80-83.
    3. Botstein, D.; White, R. L.; Skolnick, M. and Davis, R. W. (1980). Construction of a genetic linkage map in man using restriction fregment length polymorphism. American J. Human Genetics 32 : 324-331.
    4. Cornuet, J. M. and Luikart, G. (1996). Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics 144: 2001–2014.
    5. Dixit, S. P.; Verma, N. K.; Ahlawat, S. P. S.; Aggarwal, R. A. K.; Kumar, S.; Chander, R. and Singh, K. P. (2008). Molecular genetic characterization of Kutchi breed of goat. Current Science 95 (7): 946-952.
    6. Dixit, S. P.; Aggarwal, R. A. K.; Verma, N. K.; Vyas, M. K.; Rana, J.; Sharma, A. and Chander, R. (2011). Genetic variability and bottleneck analyses of Kanniadu goat breed based on microsatellite markers. Indian J. Anim. Sci. 81 (1): 40-43.
    7. Dixit, S. P.; Aggarwal, R. A. K.; Dandi P. S.; Verma, N. K.; Vyas, M. K.; Rana, J.; Sharma, A.; Kharadi, V. B.; Sabapara, G. P. and Deshpande, S. B. (2013). Phenotypic characteristics, management, performance and genetic variability in Surati breed of goat. Indian J. Anim. Sci. 83 (4) : 423-427.
    8. Di Rienzo, A.; Peterson, A. C.; Garca, J. C.; Valdes, A. M. and Slatkin, M. (1994). Mutational proceses of simple sequence repeats in human populations. Proceedings of National Academy of Sciences, USA 91: 3168–70.
    9. Kumar, D.; Dixit ,S. P.; Verma, N. K.; Singh, D. K.; Pande, A.; Kumar, S.; Chander, R. and Singh, L. B. (2009). Genetic diversity analysis of the Gohilwari breed of Indian goat (Capra hircus) using microsatellite markers. American J. Anim. Vet. Sci. 4 (3) : 49-57.
    10. Kimura, M. and Crow, J. W. (1964). The number of alleles that can be maintained in a finite population. Genetics 49: 725–738.
    11. Levene, H. (1949). On a matching problem arising in genetics. The Annals of Mathematic Statistics 20: 91–94.
    12. Mishra, P.; Verma, N. K.; Aggarwal, R. A. K. and Dixit, S. P. (2010). Breed characteristics and genetic variability in Changthangi goats. Indian J. Anim. Sci. 80 (12) : 1203–1209.
    13. Mishra, P.; Ali, S. A. and Verma, N. K. (2012). Phenotypic, biometric and genetic characterization of Bundelkhandi goats. Indian J. Anim. Sci. 82 (11) : 1442–1445.
    14. Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proceedings of National Academy of Sciences USA 70: 3321– 3323.
    15. Ohta, T. and Kimura, M. (1973). A model of mutation appropriate to estimate the number of electrophoretically detectable alleles in a finite population. Genetic Research Cambridge 22: 201–204
    16. Sambrook, J. and Russell, D. W. (2001). Molecular Cloning : A Laboratory Manual. 3rd edn. Cold Spring Harbour Cold Spring Laboratory Press NY.
    17. Shannon, C. E. and Weaver, W. (1949). The Mathematical Theory of Communication. University of Illinois Press, IL.
    18. Sharma, R.; Kumar, D.; Pandey, A. K.; Dixit, S. P. and Ahlawat, S. P. S. (2008a) . Genetic variability and bottleneck analysis of Barbari goat population using microsatellite markers. Indian J. Anim. Sci. 78 (4) : 391-396.
    19. Sharma, R.; Kumar, D.; Pandey, A. K.; Dixit, S. P. and Ahlawat, S. P. S. (2008b). Genetic variability in Beetal goat breed assessed by microsatellite markers. Indian J. Anim. Sci. 78 (5):505:509.
    20. Takezaki, N. and Nei, M. (1996) Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genetics 144: 389-399.
    21. Verma, N. K.; Dixit, S. P.; Aggarwal, R. A. K.; Chander, R.; Kumar, S. and Ahlawat, S. P. S. (2007). Molecular genetic analysis of Sirohi breed of goat. Korean J. Genet. 29: 129-136.
    22. Vijh, R K.; Tantia, M. S.; Bhel, R. and Mishra, B. (2010). Genetic architecture of Black Bengal and Chegu goat. Indian J. Anim. Sci. 80 (12): 1134-1137.
    23. Yeh, F. C.; Boyle, T.; Rongcai, Y.; Ye, Z. and Xian, J. M. (1999). POPGENE version 3.1(http://www.ualberta.ca/-fyeh/fyeh.)
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