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volume 49 issue 4 (august 2015) : 482-486,   Doi: 10.5958/0976-0555.2015.00095.3

Effect of zinc supplementation on haematology and serum biochemical constituents in Murrah buffalo calves

S
S. Parashu Ramulu
D
D. Nagalakshmi
M
M. Kishan Kumar
1Department of Animal Nutrition, College of Veterinary Science, Rajendranagar, Hyderabad-30, India.
Cite article:- Ramulu Parashu S., Nagalakshmi D., Kumar Kishan M. (2025). Effect of zinc supplementation on haematology and serum biochemical constituents in Murrah buffalo calves. Indian Journal of Animal Research. 49(4): 482-486. doi: 10.5958/0976-0555.2015.00095.3.

ABSTRACT

Eighteen buffalo calves (125±2.5 kg) were randomly allotted to 3 groups in a completely randomized design. The dietary treatments were viz., Basal diet (BD; 29.72 ppm Zn from feed ingredients), BD supplemented with 80 and 140 ppm Zn as ZnSO4.7H2O (22% Zn). The nutritional requirements were met by feeding sorghum stover based complete diets comprising of mainly sorghum stover, maize grain, soya bean meal and red gram chunni for a period of 120 days. Results showed that Zn supplementation at either 80 or 140 ppm had no influence on haematological constituents (haemoglobin, total erythrocyte and leucocytes count, packed cell volume, MCV, lymphocytes, monocytes and granulocytes) and biochemical constituents (total protein, albumin, cholesterol, glucose and albumin : globulin ratio) analyzed at 90 d of trial. Irrespective of dose, alkaline phosphatase activity and globulin concentration in serum increased (P

REFERENCES

  1. Alloway, B.J. (2008). Zinc in Soils and Crop Nutrition. 2nd edn. Brussels, Belgium, Paris and France, International Zinc Association (IZA), IFA.
  2. Arenza J.S. Hathi, S.D. Singh, B. and Verma, P.N. (1977). Status of some microminerals in neonatal buffalo calves and their mothers. Indian J. Dairy Sci. 30: 255.
  3. Cooper, G.R. and Mc Daniel, V. (1970). Assay methods. In: Mc Donald R.P. (Ed.) Standard Methods for Clinical Chemistry. John Wiley and Sons, New York. 159 – 170.
  4. Coulston, L. and Dandona, P. (1980). Insulin like effects of zinc on adipocytes. Diabetes. 29: 665–667.
  5. Gaafar, H.M.A. Bassiouni, M.I. Ali, M.F.E. Shitta, A.A. and Shamas, A.Sh.E. (2011). Effect of Zinc Methionine Supplementation on Productive Performance of Lactating Friesian Cows. J. Anim. Sci. Biotech. 2(2):94-101.
  6. Garg, A.K. Mudgal, V. and Dass, R.S. (2008). Effect of organic zinc supplementation on growth, nutrient utilization and mineral profile in lambs. Anim. Feed Sci. Technol. 144: 82-96.
  7. Gowda, N.K.S. Pal, D.T. Gupta, R. Prasad, C.S. and Sampath, K.T. (2009) Feed resources and feeding practices in different agro- eco zones of India. NIANP, Bangalore.
  8. Gustafson, E.J. (1976). Improved specificity of serum albumin determination and estimation of acute phase of reactants by use of the bromocresol green. Clin Chem.22:616-622.
  9. Hatfield, P.G. Robinson, B.L. Minikhiem, D.L. Kott, R.W. Roth, N.I. Daniels, J.T. and Swenson, C.K. (2002). Serum
  10. a-tocopherol and immune function in yearling ewes supplemented with zinc and vitamin E. J. Anim. Sci. 80:1329-1334.
  11. Jia, W.B. Jia, Z.H. Zhang, W. Wang, R.L. Zhang, S.W. and Zhu, X.P. (2008) Effects of dietary zinc on performance, nutrient digestibility and plasma zinc status in Cashmere goats. Small Rumin. Res. 80:68-72.
  12. Kaneko, J. Harvey, J. and Bruss, M. (1997). Clinical Biochemistry of Domestic Animals, 5 Ed, Academic Press,USA.
  13. Kearney, J.F. Bartles, J. Hamilton, A.M. Lehuen, A. Solvason, J. Vakil, M. (1992). Development and function of the early B cell repertoire. Int. Rev. Immunol. 8:247-57.
  14. Kind, P.R. and King, E.J. (1954). Estimation of plasma phosphatase by determination of hydrolyzed phenol with amino- antipyrine. J. Clin. Path. 7:322-326.
  15. Laar, H.V and Jongbloed, A.W. (2010). Effect of zinc and copper on health and fertility in cattle, internal report 201004, Wageningen UR Livestock Research
  16. Malcolm-Callis, K.J. Duff, G.C. Gunter, S.A. Kegley, E.B. and Vermeire, D.A. (2000). Effects of supplemental zinc concentration and source on performance, carcass characteristics, and serum values in finishing beef steers. J. Anim. Sci. 78:2801-2808.
  17. Mandal, G.P. and Dass, R.S. (2010). Haemato-biochemical profile of crossbred calves supplemented with inorganic and organic source of zinc. Indian J. Anim. Res. 44(3):197- 200.
  18. Mandal, G.P. Dass, R.S. Garg, A.K. Varshney, V.P. and Mondal, A.B. (2008). Effect of zinc supplementation from inorganic and organic sources on growth and blood biochemical profile in crossbred calves. J. Anim.Feed Sci. 17:147–156.
  19. May, J.M., and Contoreggi, C.S. (1982). The mechanism of insulin like effects of ionic zinc. J. Biol. Chem. 257:4362–4368
  20. Mc Dowell, L.R. (1992). Minerals in animal human nutrition. Academic Press, New York, p. 289.
  21. Miller, J.K. and Cragle, R.G. (1965). Gastrointestinal sites of absorption and endogenous secretion of zinc in dairy cattle. J. Dairy Sci. 48:370.
  22. Mohan, N.H. Niyogi, D. Waghaye, J.Y. and Singh, H.N. (2009). Age-related haematological changes in Murrah buffalo (Bubalus bubalis) calves. Indian J. Anim. Sci. 79(4):369–371.
  23. Nagalakshmi, D. Dhanalakshmi, K. and Himabindu, D. (2009b). Effect of dose and source of supplemental zincon immune response and oxidative enzymes in lambs. Vet. Res. Commun. 33(7):631-644.
  24. Nagalakshmi, D. Parashuramulu, S. Usha Rani, M. (2013b). Effect of graded levels of zinc supplementation on growth performance and oxidative defense mechanism in rats. IOSR J. Pharm.2 (6): 36-41.
  25. Nagalakshmi, D. Parashuramulu, S. Usha Rani, M. Kalakumar, B. Shivakumar, P. Reddy A Gopala. (2013a). Effect of zinc supplementation on immune response, haematological and serum biochemical constituents in rats. Inventi Rapid: Biomed. Anal. 1: 1-4.
  26. Nagalakshmi, D. Reddy, M.R. and Prasad, M.R. (2009a). Mineral status of dairy animals in high altitude and tribal zone of Andhra Pradesh. In: Proceedings of ANA world conference, 14th -17th Febrauary, 2009, New Delhi.
  27. NRC. (1995). Nutrient Requirements of Laboratory Animals. 4th revised edition, National Academy of Science, Washington, DC.
  28. NRC. (2001). Nutrient Requirements of Dairy Cattle. National Academy of Science, Washington, DC.
  29. Parashuramulu, S. Nagalakshmi, D. and Srinivas Rao, D. (2013). Dose response effect of dietary zinc on in vitro digestibility and gas production in buffaloes. Indian J. Anim. Nutr. 30(4):365-369
  30. Prohaska, J.R. (1991). Changes in Cu, Zn-Superoxide dismutase, Cytochrome-C oxidase, Glutathione peroxidise and Glutathione transferase activities in copper deficient mice and rats. J. Nutr. 121(3): 355-363.
  31. Reinhold, J.G. (1953). Standard methods of clinical chemistry C, (Ed.) Rynner, M, New York, Academic Press. p. 88.
  32. Rojas, L.X. Mc Dowell, L.R. Cousins, R.J. Martins, F.G. Nilkinson, N.S. Johnson, A.B. and Velasquez, J.B. (1995). Relative bioavailability of two organic and two inorganic zinc sources fed sheep. J. Anim. Sci. 73:1202-1207.
  33. Salama Ahmed, A.K, Cajat, G. Albanell, E. Snch, X. and Caslas, R, (2003). Effects of dietary supplements of zinc-methionine on milk production, udder health and zinc metabolism in dairy goats. J. Dairy Res. 70: 9-17.
  34. Shakweer, I.M.E, Mustafa, G.M. Ahmed, G.A. and Ismail, M.I.N. (2010). Effect of zinc or / and manganese methionine supplements on performance of lactating buffaloes. J. Anim. Poul. Prod. 1(11):589-602.
  35. Shinde, P.L. Garg, A.K. Das, R.S. Chaturvedi, V.K. and Kaushik, P. (2007). Effect of dietary supplementation of zinc on humoral immunity Effect of dietary supplementation of zinc on humoral immunity in guinea pigs. Indian J. Anim. Nutr. 24(1):55-58.
  36. Shisheva, A. Gefel, D. and Shechter, Y. (1992). Insulin like effects of zinc ion in vitro and in vivo: preferential effects on desensitized adipocytes and induction of normoglycemia in streptozocin-induced rats. Diabetes. 41:982-988
  37. Sung, I.K. and Dale, A.W. (1981). Relationship between the nutritional status of zinc and cholesterol concentration of serum lipoproteins in adult male rats. Clin.Nutr. 34:2376- 2381.
  38. Underwood, E.J. and Suttle, N.F. (1999). Zinc. In: The Mineral Nutrition of Livestock. 3rd Ed, New York, CABI Publishing, p: 489.
  39. Whitman, K.J. Engle, T.E. Burns, P.D. Dorton, K.L. Ahola, J.K. Enns, R.M. and Stanton, T.L. (2007). Effects of Copper and Zinc source on performance, carcass characteristics, and lipid metabolism in finishing steers. Prof. Anim. Sci. 23:36-41.
  40. Wybenga, D.R. and Pileggi, V.J. (1970). Estimation of cholesterol. Clin. Chem. 16:980.
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    volume 49 issue 4 (august 2015) : 482-486,   Doi: 10.5958/0976-0555.2015.00095.3

    Effect of zinc supplementation on haematology and serum biochemical constituents in Murrah buffalo calves

    S
    S. Parashu Ramulu
    D
    D. Nagalakshmi
    M
    M. Kishan Kumar
    1Department of Animal Nutrition, College of Veterinary Science, Rajendranagar, Hyderabad-30, India.
    Cite article:- Ramulu Parashu S., Nagalakshmi D., Kumar Kishan M. (2025). Effect of zinc supplementation on haematology and serum biochemical constituents in Murrah buffalo calves. Indian Journal of Animal Research. 49(4): 482-486. doi: 10.5958/0976-0555.2015.00095.3.

    ABSTRACT

    Eighteen buffalo calves (125±2.5 kg) were randomly allotted to 3 groups in a completely randomized design. The dietary treatments were viz., Basal diet (BD; 29.72 ppm Zn from feed ingredients), BD supplemented with 80 and 140 ppm Zn as ZnSO4.7H2O (22% Zn). The nutritional requirements were met by feeding sorghum stover based complete diets comprising of mainly sorghum stover, maize grain, soya bean meal and red gram chunni for a period of 120 days. Results showed that Zn supplementation at either 80 or 140 ppm had no influence on haematological constituents (haemoglobin, total erythrocyte and leucocytes count, packed cell volume, MCV, lymphocytes, monocytes and granulocytes) and biochemical constituents (total protein, albumin, cholesterol, glucose and albumin : globulin ratio) analyzed at 90 d of trial. Irrespective of dose, alkaline phosphatase activity and globulin concentration in serum increased (P

    REFERENCES

    1. Alloway, B.J. (2008). Zinc in Soils and Crop Nutrition. 2nd edn. Brussels, Belgium, Paris and France, International Zinc Association (IZA), IFA.
    2. Arenza J.S. Hathi, S.D. Singh, B. and Verma, P.N. (1977). Status of some microminerals in neonatal buffalo calves and their mothers. Indian J. Dairy Sci. 30: 255.
    3. Cooper, G.R. and Mc Daniel, V. (1970). Assay methods. In: Mc Donald R.P. (Ed.) Standard Methods for Clinical Chemistry. John Wiley and Sons, New York. 159 – 170.
    4. Coulston, L. and Dandona, P. (1980). Insulin like effects of zinc on adipocytes. Diabetes. 29: 665–667.
    5. Gaafar, H.M.A. Bassiouni, M.I. Ali, M.F.E. Shitta, A.A. and Shamas, A.Sh.E. (2011). Effect of Zinc Methionine Supplementation on Productive Performance of Lactating Friesian Cows. J. Anim. Sci. Biotech. 2(2):94-101.
    6. Garg, A.K. Mudgal, V. and Dass, R.S. (2008). Effect of organic zinc supplementation on growth, nutrient utilization and mineral profile in lambs. Anim. Feed Sci. Technol. 144: 82-96.
    7. Gowda, N.K.S. Pal, D.T. Gupta, R. Prasad, C.S. and Sampath, K.T. (2009) Feed resources and feeding practices in different agro- eco zones of India. NIANP, Bangalore.
    8. Gustafson, E.J. (1976). Improved specificity of serum albumin determination and estimation of acute phase of reactants by use of the bromocresol green. Clin Chem.22:616-622.
    9. Hatfield, P.G. Robinson, B.L. Minikhiem, D.L. Kott, R.W. Roth, N.I. Daniels, J.T. and Swenson, C.K. (2002). Serum
    10. a-tocopherol and immune function in yearling ewes supplemented with zinc and vitamin E. J. Anim. Sci. 80:1329-1334.
    11. Jia, W.B. Jia, Z.H. Zhang, W. Wang, R.L. Zhang, S.W. and Zhu, X.P. (2008) Effects of dietary zinc on performance, nutrient digestibility and plasma zinc status in Cashmere goats. Small Rumin. Res. 80:68-72.
    12. Kaneko, J. Harvey, J. and Bruss, M. (1997). Clinical Biochemistry of Domestic Animals, 5 Ed, Academic Press,USA.
    13. Kearney, J.F. Bartles, J. Hamilton, A.M. Lehuen, A. Solvason, J. Vakil, M. (1992). Development and function of the early B cell repertoire. Int. Rev. Immunol. 8:247-57.
    14. Kind, P.R. and King, E.J. (1954). Estimation of plasma phosphatase by determination of hydrolyzed phenol with amino- antipyrine. J. Clin. Path. 7:322-326.
    15. Laar, H.V and Jongbloed, A.W. (2010). Effect of zinc and copper on health and fertility in cattle, internal report 201004, Wageningen UR Livestock Research
    16. Malcolm-Callis, K.J. Duff, G.C. Gunter, S.A. Kegley, E.B. and Vermeire, D.A. (2000). Effects of supplemental zinc concentration and source on performance, carcass characteristics, and serum values in finishing beef steers. J. Anim. Sci. 78:2801-2808.
    17. Mandal, G.P. and Dass, R.S. (2010). Haemato-biochemical profile of crossbred calves supplemented with inorganic and organic source of zinc. Indian J. Anim. Res. 44(3):197- 200.
    18. Mandal, G.P. Dass, R.S. Garg, A.K. Varshney, V.P. and Mondal, A.B. (2008). Effect of zinc supplementation from inorganic and organic sources on growth and blood biochemical profile in crossbred calves. J. Anim.Feed Sci. 17:147–156.
    19. May, J.M., and Contoreggi, C.S. (1982). The mechanism of insulin like effects of ionic zinc. J. Biol. Chem. 257:4362–4368
    20. Mc Dowell, L.R. (1992). Minerals in animal human nutrition. Academic Press, New York, p. 289.
    21. Miller, J.K. and Cragle, R.G. (1965). Gastrointestinal sites of absorption and endogenous secretion of zinc in dairy cattle. J. Dairy Sci. 48:370.
    22. Mohan, N.H. Niyogi, D. Waghaye, J.Y. and Singh, H.N. (2009). Age-related haematological changes in Murrah buffalo (Bubalus bubalis) calves. Indian J. Anim. Sci. 79(4):369–371.
    23. Nagalakshmi, D. Dhanalakshmi, K. and Himabindu, D. (2009b). Effect of dose and source of supplemental zincon immune response and oxidative enzymes in lambs. Vet. Res. Commun. 33(7):631-644.
    24. Nagalakshmi, D. Parashuramulu, S. Usha Rani, M. (2013b). Effect of graded levels of zinc supplementation on growth performance and oxidative defense mechanism in rats. IOSR J. Pharm.2 (6): 36-41.
    25. Nagalakshmi, D. Parashuramulu, S. Usha Rani, M. Kalakumar, B. Shivakumar, P. Reddy A Gopala. (2013a). Effect of zinc supplementation on immune response, haematological and serum biochemical constituents in rats. Inventi Rapid: Biomed. Anal. 1: 1-4.
    26. Nagalakshmi, D. Reddy, M.R. and Prasad, M.R. (2009a). Mineral status of dairy animals in high altitude and tribal zone of Andhra Pradesh. In: Proceedings of ANA world conference, 14th -17th Febrauary, 2009, New Delhi.
    27. NRC. (1995). Nutrient Requirements of Laboratory Animals. 4th revised edition, National Academy of Science, Washington, DC.
    28. NRC. (2001). Nutrient Requirements of Dairy Cattle. National Academy of Science, Washington, DC.
    29. Parashuramulu, S. Nagalakshmi, D. and Srinivas Rao, D. (2013). Dose response effect of dietary zinc on in vitro digestibility and gas production in buffaloes. Indian J. Anim. Nutr. 30(4):365-369
    30. Prohaska, J.R. (1991). Changes in Cu, Zn-Superoxide dismutase, Cytochrome-C oxidase, Glutathione peroxidise and Glutathione transferase activities in copper deficient mice and rats. J. Nutr. 121(3): 355-363.
    31. Reinhold, J.G. (1953). Standard methods of clinical chemistry C, (Ed.) Rynner, M, New York, Academic Press. p. 88.
    32. Rojas, L.X. Mc Dowell, L.R. Cousins, R.J. Martins, F.G. Nilkinson, N.S. Johnson, A.B. and Velasquez, J.B. (1995). Relative bioavailability of two organic and two inorganic zinc sources fed sheep. J. Anim. Sci. 73:1202-1207.
    33. Salama Ahmed, A.K, Cajat, G. Albanell, E. Snch, X. and Caslas, R, (2003). Effects of dietary supplements of zinc-methionine on milk production, udder health and zinc metabolism in dairy goats. J. Dairy Res. 70: 9-17.
    34. Shakweer, I.M.E, Mustafa, G.M. Ahmed, G.A. and Ismail, M.I.N. (2010). Effect of zinc or / and manganese methionine supplements on performance of lactating buffaloes. J. Anim. Poul. Prod. 1(11):589-602.
    35. Shinde, P.L. Garg, A.K. Das, R.S. Chaturvedi, V.K. and Kaushik, P. (2007). Effect of dietary supplementation of zinc on humoral immunity Effect of dietary supplementation of zinc on humoral immunity in guinea pigs. Indian J. Anim. Nutr. 24(1):55-58.
    36. Shisheva, A. Gefel, D. and Shechter, Y. (1992). Insulin like effects of zinc ion in vitro and in vivo: preferential effects on desensitized adipocytes and induction of normoglycemia in streptozocin-induced rats. Diabetes. 41:982-988
    37. Sung, I.K. and Dale, A.W. (1981). Relationship between the nutritional status of zinc and cholesterol concentration of serum lipoproteins in adult male rats. Clin.Nutr. 34:2376- 2381.
    38. Underwood, E.J. and Suttle, N.F. (1999). Zinc. In: The Mineral Nutrition of Livestock. 3rd Ed, New York, CABI Publishing, p: 489.
    39. Whitman, K.J. Engle, T.E. Burns, P.D. Dorton, K.L. Ahola, J.K. Enns, R.M. and Stanton, T.L. (2007). Effects of Copper and Zinc source on performance, carcass characteristics, and lipid metabolism in finishing steers. Prof. Anim. Sci. 23:36-41.
    40. Wybenga, D.R. and Pileggi, V.J. (1970). Estimation of cholesterol. Clin. Chem. 16:980.
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