Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 49 issue 3 (june 2015) : 317-319,   Doi: 10.5958/0976-0555.2015.00067.9

Growing follicle in buffalo ovary: A histomorphometrical study during different seasons of the year

J
Jasvinder Singh Sasan*
V
Varinder Uppal
N
Neelam Bansal
1Department of Veterinary Anatomy, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana– 141 004, India.
Cite article:- Sasan* Singh Jasvinder, Uppal Varinder, Bansal Neelam (2025). Growing follicle in buffalo ovary: A histomorphometrical study during different seasons of the year. Indian Journal of Animal Research. 49(3): 317-319. doi: 10.5958/0976-0555.2015.00067.9.

ABSTRACT

The present study was conducted on 100 ovaries of adult buffaloes collected from slaughter house during different seasons of year viz; winter, spring, summer, rainy and autumn. The whole ovary was processed for paraffin blocks preparation and sectioned with a rotary microtome at a thickness of 10micron. The growing follicles were characterized by an oocyte surrounded by 2 or more layers of granulosa cells enclosed with a basement membrane. Three types of growing follicles were observed. Type I was without any connective tissue layer. Type II was surrounded by connective tissue layer but without any antrum whereas Type III was having connective tissue layer and small pools of antrum. There was no significant difference in follicular diameter during different season. The average number of growing follicles was significantly higher.

REFERENCES

  1. Abercrombie M. (1946). Estimation of nuclear population from microstructure sections.Acta Rec 94: 236-47.
  2. Bansal N. (2002). ‘Gross and histomorphological studies on follicular population in ovaries of buffalo (Bubalus bubalis)’. Ph.D. dissertation.Punjab Agricultural University Ludhiana, India.
  3. Bhardwaj R L and Roy K S. (2004). Follicular development and micrometry in the ovary of prepubertal Indian buffalo (Bubalus bubalis). Indian Journal of Animal Sciences 74(12): 1208-12.
  4. Copenhaver W M, Kelly D E and Richard L W. (1978). Bailey’s Text Book of Histology. 17thEdn, pp.629-30. William and Wilkins Co., Baltimore.
  5. Danell B. (1987). ‘Oestrus behavior, ovarian morphology and cyclical variation in follicular system and endocrine pattern in water buffalo heifers’. Swedish University of Agricultural Science, Uppsala, Sweden.
  6. Goswami R K. (1985). ‘Histomorphological and histochemical observations on ovary in Murrah buffaloes’.M.V.Sc. Thesis, Haryana Agricultural University, Hissar, India.
  7. Harrison R J and Wier B J. (1977). Structure of mammalian ovary. In: Professor Lord Zuckerman and Barbara J Wier (Ed) Vol l The Ovary.pp 113-218. Academic Press, New York.
  8. Luna, L.G. (1968). Manual of histological staining methods of Armed Forces Institute of Pathology. 3rd Ed. McGraw Hill Book Company, New York, pp 34-157.
  9. Madan, M.L. (1988). Status of reproduction in female buffalo. In: Buffalo Production and Health : A Compendium of Latest Research Information Based on Indian Studies. ICAR publication, New Delhi, India.
  10. Miyamura M, Kuwayama M, Hamawaki A and Eguchil Y. (1996). Total number of follicles in the ovaries of Japanese black cattle. Theriogenology 45: 300.
  11. Mondadori R G, Luque M C A, Santin and Bao S N. (2007). Ultrastructural and morphometric characterization of buffalo (Bubalus bubalis) ovarian preantral follicles. Animal Reproduction Science 97: 323-33.
  12. Prasad G, Roy M K and Singh L P. (1979). Histological and certain histochemical observations on the ovary of adult Indian buffaloes (Bubalus bubalis). Indian Journal of Animal Sciences 49: 184-90.
  13. Shah S N H. (1988). Comparative studies of seasonal influence on breeding behavior and conception rate of dairy buffalo and zebu cattle. Proceedings of 11th Congress on Animal Reproduction and Artificial Insemination 3 : 538.
  14. Taneja, M., Toey, S.M., Pawshe, C.H., Singh, G., Alli, A. and Talwar, G.P. (1991). Proceedingsof Third World Buffalo Congress. May 13-17, Vol. 3, pp. 581-85. Varna Bulgaria.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 49 issue 3 (june 2015) : 317-319,   Doi: 10.5958/0976-0555.2015.00067.9

    Growing follicle in buffalo ovary: A histomorphometrical study during different seasons of the year

    J
    Jasvinder Singh Sasan*
    V
    Varinder Uppal
    N
    Neelam Bansal
    1Department of Veterinary Anatomy, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana– 141 004, India.
    Cite article:- Sasan* Singh Jasvinder, Uppal Varinder, Bansal Neelam (2025). Growing follicle in buffalo ovary: A histomorphometrical study during different seasons of the year. Indian Journal of Animal Research. 49(3): 317-319. doi: 10.5958/0976-0555.2015.00067.9.

    ABSTRACT

    The present study was conducted on 100 ovaries of adult buffaloes collected from slaughter house during different seasons of year viz; winter, spring, summer, rainy and autumn. The whole ovary was processed for paraffin blocks preparation and sectioned with a rotary microtome at a thickness of 10micron. The growing follicles were characterized by an oocyte surrounded by 2 or more layers of granulosa cells enclosed with a basement membrane. Three types of growing follicles were observed. Type I was without any connective tissue layer. Type II was surrounded by connective tissue layer but without any antrum whereas Type III was having connective tissue layer and small pools of antrum. There was no significant difference in follicular diameter during different season. The average number of growing follicles was significantly higher.

    REFERENCES

    1. Abercrombie M. (1946). Estimation of nuclear population from microstructure sections.Acta Rec 94: 236-47.
    2. Bansal N. (2002). ‘Gross and histomorphological studies on follicular population in ovaries of buffalo (Bubalus bubalis)’. Ph.D. dissertation.Punjab Agricultural University Ludhiana, India.
    3. Bhardwaj R L and Roy K S. (2004). Follicular development and micrometry in the ovary of prepubertal Indian buffalo (Bubalus bubalis). Indian Journal of Animal Sciences 74(12): 1208-12.
    4. Copenhaver W M, Kelly D E and Richard L W. (1978). Bailey’s Text Book of Histology. 17thEdn, pp.629-30. William and Wilkins Co., Baltimore.
    5. Danell B. (1987). ‘Oestrus behavior, ovarian morphology and cyclical variation in follicular system and endocrine pattern in water buffalo heifers’. Swedish University of Agricultural Science, Uppsala, Sweden.
    6. Goswami R K. (1985). ‘Histomorphological and histochemical observations on ovary in Murrah buffaloes’.M.V.Sc. Thesis, Haryana Agricultural University, Hissar, India.
    7. Harrison R J and Wier B J. (1977). Structure of mammalian ovary. In: Professor Lord Zuckerman and Barbara J Wier (Ed) Vol l The Ovary.pp 113-218. Academic Press, New York.
    8. Luna, L.G. (1968). Manual of histological staining methods of Armed Forces Institute of Pathology. 3rd Ed. McGraw Hill Book Company, New York, pp 34-157.
    9. Madan, M.L. (1988). Status of reproduction in female buffalo. In: Buffalo Production and Health : A Compendium of Latest Research Information Based on Indian Studies. ICAR publication, New Delhi, India.
    10. Miyamura M, Kuwayama M, Hamawaki A and Eguchil Y. (1996). Total number of follicles in the ovaries of Japanese black cattle. Theriogenology 45: 300.
    11. Mondadori R G, Luque M C A, Santin and Bao S N. (2007). Ultrastructural and morphometric characterization of buffalo (Bubalus bubalis) ovarian preantral follicles. Animal Reproduction Science 97: 323-33.
    12. Prasad G, Roy M K and Singh L P. (1979). Histological and certain histochemical observations on the ovary of adult Indian buffaloes (Bubalus bubalis). Indian Journal of Animal Sciences 49: 184-90.
    13. Shah S N H. (1988). Comparative studies of seasonal influence on breeding behavior and conception rate of dairy buffalo and zebu cattle. Proceedings of 11th Congress on Animal Reproduction and Artificial Insemination 3 : 538.
    14. Taneja, M., Toey, S.M., Pawshe, C.H., Singh, G., Alli, A. and Talwar, G.P. (1991). Proceedingsof Third World Buffalo Congress. May 13-17, Vol. 3, pp. 581-85. Varna Bulgaria.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)