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volume 49 issue 3 (june 2015) : 320-324,   Doi: 10.5958/0976-0555.2015.00061.8

Genetic characterisation of Tiruchy Black sheep of Tamil Nadu using microsatellite markers

S
S.T. Kavitha
A
A. Subramanian
S
S.N. Sivaselvam*
R
R. Thiagarajan
S
S. Balasubramanian
1Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai-600 007, India.
Cite article:- Kavitha S.T., Subramanian A., Sivaselvam* S.N., Thiagarajan R., Balasubramanian S. (2025). Genetic characterisation of Tiruchy Black sheep of Tamil Nadu using microsatellite markers. Indian Journal of Animal Research. 49(3): 320-324. doi: 10.5958/0976-0555.2015.00061.8.

ABSTRACT

A total of 203 alleles were observed in the sampled population for the 25 marker loci under investigation. The number of alleles observed at each locus varied from 4 (OarHH41) to 13 (OarHH35) with a mean of 8.12±0.45 across all loci. The effective number of alleles ranged from 1.59 (OarHH41) to 7.51 (OarHH35). The mean number of effective alleles was 4.48±0.27 across all loci. The polymorphic information content values ranged from 0.7029 (OarHH41) to 0.9164 (OarHH35) with a mean of 0.8441±0.012, which showed high level of genetic variability. The x2 test revealed that among the 25 microsatellites studied only ten were in Hardy-Weinberg Equilibrium proportions. The observed heterozygosity ranged from 0.2609 (OarHH41) to 0.9600 (OarCP49) with a mean of 0.6995 and the expected heterozygosity ranged from 0.3734 (OarHH41) to 0.8756 (OarHH35) with a mean of 0.7575. The within population inbreeding estimates or heterozygotes deficiency within population (FIS) was observed in 13 loci and the positive FIS value ranged from 0.0796 (CSSM31) to 0.5895 (OarJMP8). Only twelve loci revealed negative FIS values (FIS

REFERENCES

  1. Arora, R. and Bhatia,S. (2004). Genetic structure of Muzzafarnagri sheep based on microsatellite analysis. Small Rum.Res., 54: 227-230.
  2. Arora, R. and Bhatia,S. (2006). Genetic diversity of Magra sheep from India using microsatellite analysis. Asian-Austr. J.Anim.Sci., 19: 938-942.
  3. Arora, R., Bhatia,S., Sehrawat,A., Maity,S.B. and Kundu,S.S. (2008). Genetic variability in Jalauni sheep of India inferred from microsatellite data. LRRD Article No. 4 (http://www.lrrd.org/lrrrd20/1/ aror20004.htm).
  4. Bhatia, S. and Arora,R. (2008). Genetic diversity in Kheri-A pastrolists developed Indian sheep using microsatellite markers. Indian J.Biotech., 7: 108-112.
  5. Botstein, D., White, R.L., Skolnick, M. and Davis, R.W. (1980). Construction of genetic linkage map in human using restriction fragment length polymorphism. Am.J.Human Genet., 32: 314-331.
  6. Bozzi, R., Degl’Innocenti, P., Rivera Diaz, P., Nardi, L., Crovetti, A., Sargentini, C., Giorgetti, A. (2009). Genetic characterization and breed assignment in five Italian sheep breeds using microsatellite markers. Small Rum.Res., 85: 50–57.
  7. Cornuet, J.M. and Luikart, G. (1996). Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics 144: 2001-2014.
  8. Dalvit, C., Sacca, E., Cassandro, M., Gervaso, M., Pastore, E. and Piasentier, E. (2008). Genetic characterization of Alpine sheep breeds. Acta Agric.Slovenica, supplement 2: 79 – 84.
  9. Girish, H., Sivaselvam, S.N., Karthickeyan, S.M.K. and Saravanan, R. (2007). Molecular characterization of Nilagiri sheep (Ovis aries) of South India based on microsatellites. Asian-Austr.J.Anim.Sci., 20: 633-637.
  10. Gutierrez-Gil, B., Metehan uzun, Juan-Jose Arranz, Fermin San Peimirivo, Swdat Yildiz, Metin Cenesiz and Yolanda Bayon. (2006). Genetic diversity in Turkish sheep. Acta.Agric. Scand., Section A, 56: 1-7.
  11. Kim, K.S, Yeo, J.S., Lee, J.W., Kim, J.W. and Choi, C.B. (2002). Genetic diversity of goats from Korea and China using microsatellite analysis. Asian-Austr.J.Anim.Sci., 4: 461-465.
  12. Kumarasamy, P., Prema, S., Ganapathi, P., Karthickeyan, S.M.K. and Kanakaraj, P. (2009). Molecular characterization of Coimbatore breed of sheep (Ovis aries) in South India. The ICFAI Univ.J.Genet.Evol., 2 (3).
  13. Mukesh, M., Sodhi, M. and Bhatia, S. (2006). Microsatellite-based diversity analysis and genetic relationships of three Indian sheep breeds. J.Anim.Breed.Genet., 123: 258-264.
  14. Peter, C., Bruford, M., Perez, T., Dalamitra, S., Hewitt, G., Erhardt, G. and the ECONOGENE Consortium. (2007). Genetic diversity and subdivision of 57 European and Middle-Eastern sheep breeds. Anim.Genet., 38: 37-44.
  15. Pramod, S., Kumarasamy, P., Rosalyn Mary Chandra, A., Sridevi, P. and Rahumathulla, P.S. (2009). Molecular characterization of Vembur sheep (Ovis aries) of south India based on microsatellites. Indian J.Sci.Tech., 2 (11).
  16. Prema, S., Sivaselvam, S.N. and Karthickeyan, S.M.K. (2008a). A note on genetic analysis in Madras Red sheep (Ovis aries) of India using microsatellite markers. LRRD. 20, Article #181 (http://www.lrrd.org/lrrd20/11/ prem20181.htm).
  17. Prema, S., Sivaselvam, S.N. and Karthickeyan, S.M.K. (2008b). Evaluation of genetic diversity in Mecheri sheep (Ovis aries) of Tamil Nadu using microsatellite markers. Indian J.Biotech., 7: 401-403.
  18. Radha, P., Sivaselvam, S.N., Kumarasamy, P. and Kumanan, K. (2011). Genetic diversity and bottleneck analysis of Kilakarsal sheep by microsatellite markers. Indian J.Biotech., 10:52-55.
  19. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbour Laboratory Press, Cold Spring Harbour, USA.
  20. Sodhi, M., Mukesh, M. and Bhatia, S. (2006). Characterising Nali and Chokla sheep differentiation with microsatellite markers. Small Rum.Res., 65(3): 185-92.
  21. Sodhi, M., Mukesh, M., Arora, R., Tantia, M.S. and Bhatia, S. (2003). Genetic structure of Garole – An unique Indian microsheep assessed using microsatellite markers. Indian J.Dairy Sci., 56: 67-173.
  22. Tapio, I., Tapio, M., Grislis, Z., Holm, L.E., Jeppsson, S., Kantanen, J., Miceikiene, I., Olsaker,I., Viinalass, H. and Eythorsdottir, E. (2005). Unfolding of population structure in Baltic sheep breeds using microsatellite analysis. Heredity, 94: 448-456.
  23. Wafula, P.O., Jianlin, H., Sangare, H., Sowe, J.M., Coly, R., Diallo, B. and Hanotte, O. (2005). Genetic characterisation of West African Djallonke sheep using microsatellite markers. The Role of Biotech., 5-7:177-178.
  24. Yeh, F.C., Boyle, T., Rongcai, Y., Ye, Z. and Xian, J.M. (1999). Popgene, Version 1.31. A Microsoft Windows based freeware for population genetic analysis. University of Alberta, Edmonton.
  25. Zhong, T., Han, J.L., Guo, J., Zhao, Q.J., Fu, B.L., He, X.H., Jeon, J.T., Guan, W.J. and Ma, Y.H. (2010). Genetic diversity of Chinese indigenous sheep breeds inferred from microsatellite markers. Small Rum.Res.,90: 88–94.
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    volume 49 issue 3 (june 2015) : 320-324,   Doi: 10.5958/0976-0555.2015.00061.8

    Genetic characterisation of Tiruchy Black sheep of Tamil Nadu using microsatellite markers

    S
    S.T. Kavitha
    A
    A. Subramanian
    S
    S.N. Sivaselvam*
    R
    R. Thiagarajan
    S
    S. Balasubramanian
    1Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai-600 007, India.
    Cite article:- Kavitha S.T., Subramanian A., Sivaselvam* S.N., Thiagarajan R., Balasubramanian S. (2025). Genetic characterisation of Tiruchy Black sheep of Tamil Nadu using microsatellite markers. Indian Journal of Animal Research. 49(3): 320-324. doi: 10.5958/0976-0555.2015.00061.8.

    ABSTRACT

    A total of 203 alleles were observed in the sampled population for the 25 marker loci under investigation. The number of alleles observed at each locus varied from 4 (OarHH41) to 13 (OarHH35) with a mean of 8.12±0.45 across all loci. The effective number of alleles ranged from 1.59 (OarHH41) to 7.51 (OarHH35). The mean number of effective alleles was 4.48±0.27 across all loci. The polymorphic information content values ranged from 0.7029 (OarHH41) to 0.9164 (OarHH35) with a mean of 0.8441±0.012, which showed high level of genetic variability. The x2 test revealed that among the 25 microsatellites studied only ten were in Hardy-Weinberg Equilibrium proportions. The observed heterozygosity ranged from 0.2609 (OarHH41) to 0.9600 (OarCP49) with a mean of 0.6995 and the expected heterozygosity ranged from 0.3734 (OarHH41) to 0.8756 (OarHH35) with a mean of 0.7575. The within population inbreeding estimates or heterozygotes deficiency within population (FIS) was observed in 13 loci and the positive FIS value ranged from 0.0796 (CSSM31) to 0.5895 (OarJMP8). Only twelve loci revealed negative FIS values (FIS

    REFERENCES

    1. Arora, R. and Bhatia,S. (2004). Genetic structure of Muzzafarnagri sheep based on microsatellite analysis. Small Rum.Res., 54: 227-230.
    2. Arora, R. and Bhatia,S. (2006). Genetic diversity of Magra sheep from India using microsatellite analysis. Asian-Austr. J.Anim.Sci., 19: 938-942.
    3. Arora, R., Bhatia,S., Sehrawat,A., Maity,S.B. and Kundu,S.S. (2008). Genetic variability in Jalauni sheep of India inferred from microsatellite data. LRRD Article No. 4 (http://www.lrrd.org/lrrrd20/1/ aror20004.htm).
    4. Bhatia, S. and Arora,R. (2008). Genetic diversity in Kheri-A pastrolists developed Indian sheep using microsatellite markers. Indian J.Biotech., 7: 108-112.
    5. Botstein, D., White, R.L., Skolnick, M. and Davis, R.W. (1980). Construction of genetic linkage map in human using restriction fragment length polymorphism. Am.J.Human Genet., 32: 314-331.
    6. Bozzi, R., Degl’Innocenti, P., Rivera Diaz, P., Nardi, L., Crovetti, A., Sargentini, C., Giorgetti, A. (2009). Genetic characterization and breed assignment in five Italian sheep breeds using microsatellite markers. Small Rum.Res., 85: 50–57.
    7. Cornuet, J.M. and Luikart, G. (1996). Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics 144: 2001-2014.
    8. Dalvit, C., Sacca, E., Cassandro, M., Gervaso, M., Pastore, E. and Piasentier, E. (2008). Genetic characterization of Alpine sheep breeds. Acta Agric.Slovenica, supplement 2: 79 – 84.
    9. Girish, H., Sivaselvam, S.N., Karthickeyan, S.M.K. and Saravanan, R. (2007). Molecular characterization of Nilagiri sheep (Ovis aries) of South India based on microsatellites. Asian-Austr.J.Anim.Sci., 20: 633-637.
    10. Gutierrez-Gil, B., Metehan uzun, Juan-Jose Arranz, Fermin San Peimirivo, Swdat Yildiz, Metin Cenesiz and Yolanda Bayon. (2006). Genetic diversity in Turkish sheep. Acta.Agric. Scand., Section A, 56: 1-7.
    11. Kim, K.S, Yeo, J.S., Lee, J.W., Kim, J.W. and Choi, C.B. (2002). Genetic diversity of goats from Korea and China using microsatellite analysis. Asian-Austr.J.Anim.Sci., 4: 461-465.
    12. Kumarasamy, P., Prema, S., Ganapathi, P., Karthickeyan, S.M.K. and Kanakaraj, P. (2009). Molecular characterization of Coimbatore breed of sheep (Ovis aries) in South India. The ICFAI Univ.J.Genet.Evol., 2 (3).
    13. Mukesh, M., Sodhi, M. and Bhatia, S. (2006). Microsatellite-based diversity analysis and genetic relationships of three Indian sheep breeds. J.Anim.Breed.Genet., 123: 258-264.
    14. Peter, C., Bruford, M., Perez, T., Dalamitra, S., Hewitt, G., Erhardt, G. and the ECONOGENE Consortium. (2007). Genetic diversity and subdivision of 57 European and Middle-Eastern sheep breeds. Anim.Genet., 38: 37-44.
    15. Pramod, S., Kumarasamy, P., Rosalyn Mary Chandra, A., Sridevi, P. and Rahumathulla, P.S. (2009). Molecular characterization of Vembur sheep (Ovis aries) of south India based on microsatellites. Indian J.Sci.Tech., 2 (11).
    16. Prema, S., Sivaselvam, S.N. and Karthickeyan, S.M.K. (2008a). A note on genetic analysis in Madras Red sheep (Ovis aries) of India using microsatellite markers. LRRD. 20, Article #181 (http://www.lrrd.org/lrrd20/11/ prem20181.htm).
    17. Prema, S., Sivaselvam, S.N. and Karthickeyan, S.M.K. (2008b). Evaluation of genetic diversity in Mecheri sheep (Ovis aries) of Tamil Nadu using microsatellite markers. Indian J.Biotech., 7: 401-403.
    18. Radha, P., Sivaselvam, S.N., Kumarasamy, P. and Kumanan, K. (2011). Genetic diversity and bottleneck analysis of Kilakarsal sheep by microsatellite markers. Indian J.Biotech., 10:52-55.
    19. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbour Laboratory Press, Cold Spring Harbour, USA.
    20. Sodhi, M., Mukesh, M. and Bhatia, S. (2006). Characterising Nali and Chokla sheep differentiation with microsatellite markers. Small Rum.Res., 65(3): 185-92.
    21. Sodhi, M., Mukesh, M., Arora, R., Tantia, M.S. and Bhatia, S. (2003). Genetic structure of Garole – An unique Indian microsheep assessed using microsatellite markers. Indian J.Dairy Sci., 56: 67-173.
    22. Tapio, I., Tapio, M., Grislis, Z., Holm, L.E., Jeppsson, S., Kantanen, J., Miceikiene, I., Olsaker,I., Viinalass, H. and Eythorsdottir, E. (2005). Unfolding of population structure in Baltic sheep breeds using microsatellite analysis. Heredity, 94: 448-456.
    23. Wafula, P.O., Jianlin, H., Sangare, H., Sowe, J.M., Coly, R., Diallo, B. and Hanotte, O. (2005). Genetic characterisation of West African Djallonke sheep using microsatellite markers. The Role of Biotech., 5-7:177-178.
    24. Yeh, F.C., Boyle, T., Rongcai, Y., Ye, Z. and Xian, J.M. (1999). Popgene, Version 1.31. A Microsoft Windows based freeware for population genetic analysis. University of Alberta, Edmonton.
    25. Zhong, T., Han, J.L., Guo, J., Zhao, Q.J., Fu, B.L., He, X.H., Jeon, J.T., Guan, W.J. and Ma, Y.H. (2010). Genetic diversity of Chinese indigenous sheep breeds inferred from microsatellite markers. Small Rum.Res.,90: 88–94.
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