Molecular characterization of Pulikulam cattle using microsatellite markers

DOI: 10.5958/0976-0555.2015.00007.2    | Article Id: B-2568 | Page : 36-39
Citation :- Molecular characterization of Pulikulam cattle using microsatellite markers.Indian Journal Of Animal Research.2015.(49):36-39
A. Barani*, P.S. Rahumathulla, R. Rajendran, P. Kumarasamy, P.Ganapathi and P. Radha barani.agb@gmail.com
Address : Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai- 600 007, India.

Abstract

The present study estimates genetic variability in Pulikulam cattle (n=50), a draught breed of India, by use of 18 microsatellite markers recommended by the Food and Agriculture Organization. Microsatellite genotypes were derived and allelic and genotypic frequencies, heterozygosities and gene diversity were estimated. A total of 142 alleles were distinguished by the 18 microsatellite markers used. All the microsatellites were highly polymorphic, with mean (± s.e.) allelic number of 7.89 ± 0.72, ranging 3-12 per locus. The observed heterozygosity in the population ranged between 0.1316 and 0.8958, with mean (± s.e.) of 0.5758 ± 0.0535, indicating considerable genetic variation in this population. Genetic bottleneck hypotheses were also explored. Our data suggest that the Pulikulam breed has not experienced a genetic bottleneck in the recent past.

Keywords

Genetic bottleneck Genetic diversity Microsatellite markers Pulikulam cattle.

References

  1. Almeida, S.E.M.; Machado, M.S.N. and Steigleder, C.S.(2000) Genetic diversity in a Brazilian bovine herd based on four microsatellite markers. Genetics and Molecular Biology, 23:347-350.
  2. Anonymous. (2004) 17th Quinquennial Livestock Census - 2004. Commissioner and Director of Veterinary Services, Government of Tamilnadu, Chennai - 6, India.
  3. Cornuet, J.M. and Luikart, G. (1996) Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics, 144:2001-2014.
  4. Food and Agricultural Organization of the United Nations. (2000) Global project for the maintenance of domestic animal genetic diversity (MoDAD).(http://www.fao.org/dad-is/).
  5. Ganapathi,P. (2007) Genetic Characterisation of Bargur Cattle. M.V.Sc. Thesis submitted to the Tamilnadu Veterinary and Animal Sciences University, Chennai, India.
  6. Hanotte,O. and Jianlin,H. (2005) Genetic characterization of livestock populations and its use in conservation decision-    making. The Role of Biotechnology, Italy, March, 5-7:131-136.
  7. Karthickeyan,S.M.K.; Saravanan,R. and Thangaraju,P. (2006) Characterization of Krishna Valley breed of cattle (Bos indicus) in South India using microsatellite markers. Livestock Research for Rural Development., 18:11. Article #151 Retrieved July 13, 2009, from http://www.lrrd.org/lrrd18/11/ kart18151.htm.
  8. Karthickeyan,S.M.K.; Sivaselvam, S.N.; Selvam, R.; Raja, T.V.; Rajendran, R. and Thangaraju,P. (2007) Umblachery breed of cattle in South India: a genetic assessment through microsatellite markers. Asian Journal of Animal Veterinary Advances, 2:218-222.
  9. Koreth,J.; Jhon, L. and James, M. (1996) Microsatellites and PCR genomics analysis. Journal of Pathology, 178:239-248.
  10. Kumar,S.; Kothekar, M.D.; Karthickeyan, S.M.K.; Saravanan, R. and Thangaraju, P. (2009) Assessment of genetic variability in Krishna Valley cattle using microsatellite markers. National Symposium on Livestock Biodiversity Conservation and Utilization: Lessons from Past and Future Perspectives, Karnal, February, 12-13:222.
  11. Kumar,S.N., Jayashankar, M.R., Nagaraja, C.S., Govindaiah, M.G., Saravanan, R. and Karthickeyan, S.M.K. (2006) Molecular characterization of Hallikar breed of cattle using microsatellite markers. Asian-Australian Journal of Animal Sciences, 19:622-626.
  12. Littlewood, R.W. (1936) Livestock of Southern India. Government of Madras, Madras. pp.51-69.
  13. Luikart, G.; Allendorf, F.W.; Cornuet, J.M. and Sherwin, W.B. (1998) Distortion of allele frequency distributions provides a test for recent population bottlenecks. Journal of Heredity, 89:238-247.
  14. Manjunatha Prabhu, B. H. (2004) Phenotypic and Molecular Genetic Characterization of Amrithmahal Cattle. M.V.Sc. Thesis, University of Agricultural Sciences, Bangalore, India.
  15. Martin-Burriel, I.; Garcia-Murao, I. and Zaragoza (1999) Genetic diversity analysis of six Spanish native cattle breeds using microsatellites. Animal Genetics, 3:177-182.
  16. Mukesh, M.; Sodhi, M.; Bhatia, S. and Mishra, B.P. (2004) Genetic diversity of Indian native cattle breeds as analysed with 20 microsatellite loci. Journal of Animal Breeding and Genetics, 121:416-424.
  17. Nei, M. (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89:583-590.
  18. Pattabhiraman, D. (1958) Breeds of Cattle in Tamilnadu. Director of Animal Husbandry, Madras.
  19. Rajendran, R.; Kanakaraj, P.; Kumarasamy, P. and Kathiresan, D (2008) Pulikulam cattle – Characteristics and its Unique Utilities. National Symposium on Redefining Role of Indigenous Animal Genetic Resources in Rural Development, Bangalore, February, 15-16 :155-156.
  20. Sambrook, J.; Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: a laboratory manual, Cold Spring Harbour Laboratory Press, Cold Spring Harbour, USA.
  21. Yeh, F.C.; Boyle, T.; Rongcai, Y.; Ye, Z. and Xian, J.M. (1999) Popgene, Version 1.31. A Microsoft Windows based Freeware for Population Genetic Analysis. University of Alberta, Edmonton.

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