Molecular characterization of 3’ UTR of Nramp1 gene in Gaolao breed of cattle

DOI: 10.5958/0976-0555.2015.00006.0    | Article Id: B-2551 | Page : 31-35
Citation :- Molecular characterization of 3’ UTR of Nramp1 gene in Gaolao breed of cattle.Indian Journal Of Animal Research.2015.(49):31-35
Rakesh Ranjan*, C.D. Bhong, S.N.S. Parmar and C.G. Joshi rkrbiotech@gmail.com
Address : Animal Biotechnology Centre, NDVSU, Jabalpur-482 001, India.

Abstract

The Nramp1 (Natural resistances macrophage protein1) gene or recently renamed Slc11a1 (solute carrier family 11 member1) is a member of a large family of genes coding for metal ion-transporting proteins. Nramp1 gene plays a critical role in innate immunity favoring bacterial killing by macrophages in addition to its influence on adaptative immunity. The aim of the present investigation was to identify the genetic variations in the 3’UTR (Untranslated region) of Nramp1 gene in the Gaolao breed (Bos indicus) cattle, using the technique PCR-SSCP and by sequencing. PCR-SSCP (Single Strand conformational polymorphism) of 440 bp amplicon of Nramp1 gene revealed four common SSCP patterns in Gaolao breed. The chi-square test revealed that difference between observed frequencies of different patterns in Gaolao was non-significant at 5% level of significance. The sequence analysis of Nramp1 region did not revealed sequence variation.  The sequence analysis of Nramp1 region bare only two sequence variation in four SSCP patterns. This indicates that this region is either heterologous or SSCP could be independent of sequence variation.

Keywords

Cattle Nramp1 gene Sequencing SSCP 3’UTR.

References

  1. Bellamy R, Ruwende C, Corrah T, McAdam K P, Whittle H, Hill A V (1998) Variations in the NRAMP1 gene and susceptibility to tuberculosis in West Africa, N. Engl. J. Med, 338: 640 – 644.
  2. Borriello G, Capparelli R, Bianco M, Fenizia D, Alfano F, Capuano F, Ercolini D, Parisi A, Roperto S, Iannelli D, (2006) Genetic resistance to Brucella abortus in the water buffalo (Bubalus bubalis), Infect. Immun,74: 2115 – 2120.
  3. Burge E J, Gauthier D T, Ottinger C A, Veld P A V, (2004) Mycobacterium-Inducible Nramp in Striped Bass (Morone saxatilis). Inf. Immun, 72: 1626 – 1636.
  4. Dekker, J C M, (1998) Optimizing strategies for selection on major genes. 47th Annual National Breeders Round table procedding. May 7-8, Missouri, America.
  5. Gruenheid S, Pinner E, Desjardins M, Gros P, (1997) Natural resistance to infection with intracellular pathogens: the Nramp1 protein is recruited to the membrane of the phagosome, J. Exp. Med, 185: 717 – 730.
  6. Gruenheid S, Gros P, (2000) Genetic susceptibility to intracellular infections: Nramp1, macrophage function and divalent cations transport, Curr. Opin. Microbiol 3: 43 – 48.
  7. John S W, Weitzner G, Rozen R, Scriver C R0, (1991) A rapid procedure for extracting genomic DNA from leukocytes. Nucleic Acids Res 19: 408
  8. Kumar N, Mitra A, Ganguly I, Singh R, Deb S M, Srivastava S K , Sharma A, (2005) Lack of association of brucellosis resistance with (GT)(13) microsatellite allele at 3’UTR of NRAMP1 gene in Indian zebu (Bos indicus) and crossbred (Bos indicus · Bos taurus) cattle. Vet. Microbiol, 111: 139 – 143.
  9. Martinez R, Dunner S, Barrera G, Canon J, (2008) Novel variants within the coding regions of the Slc11A1 gene identified in Bos taurus and Bos indicus breeds, J. Anim. Breed. Genet, 125: 57 – 62.
  10. Paixao T A, Ferreira C, Borges A M, Oliveira D A A, Lage A P, Santos R L, (2006) Frequency of bovine Nramp1 (Slc11a1) allelesin Holstein and Zebu breeds, Vet. Immunol. Immunopath 109: 37 – 42.
  11. Paixao T A, Poester F P, Neta A V C, Borges A M, Lage A P, Santos R L, (2007) Nramp1 3’untranslated region Polymorphism are not associated with Natural Resistance to Brucella abortus in cattle. Infect. Immun. 75: 2493 – 2499.
  12. Rakesh Ranjan, Chandrakant D. Bhong, Khagol N. Chavan, S. N. S. Parmar, Chaitanya G. Joshi, (2011) Polymorphism in 3’UTR Region of Slc11a1 Gene in Indian Breeds of Cattle, VETERINARSKI ARHIV 81: 349-357.
  13. Sambrook J & Russell W, (2001) Molecular Cloning: A Laboratory Manual, Cold Spring Harbour Laboratory (Cold Spring Harbour, New York)
  14. Wyllie S, Seu J A, Goss P, (2002) The natural resistance-associated macrophage protein1 Slc11a1 (formerly Nramp1) and iron metabolism in macrophages, Microbes. Infect 4: 351 – 359.

Global Footprints