Immunological profiles of lactating and mastitis crossbred cattle

ABSTRACT

In order to assess the immune profiles of lactating and mastitis crossbred cows, blood samples were collected weekly for one month from early, mid and late lactating cows and once from clinical and subclinical mastitis cows. Immune markers nitric oxide, tumor necrosis factor a and immunoglobulin G were estimated in blood plasma. A concurrent significant (P<0.05) fall in IgG concentration was noted in clinical mastitis cows and was highest during mid lactation in lactating cows. The clinical and subclinical mastitis cows had significantly (P<0.05) high levels of TNF-a and were non-detectable in lactating cows. Plasma NO level exhibited a massive rise (P<0.05) in mastitis cows (clinical and subclinical), the levels were significantly (P<0.05) higher than in the normal lactating states.

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REFERENCES

Blum,J.W., Dosogne, H., Hoeben, D., Vangroenweghe, F., Hammon, H.M., Bruckmaier, R.M. and Burvenich, C. (2000). Tumor necrosis factor-alpha and nitrite/nitrate responses during acute mastitis induced by Escherichia coli infection and endotoxin in dairy cows. Domest. Anim. Endocrinol. 19: 223– 235.

Bray (2011). Producing Quality Milk in Florida, Proceedings 47th Florida Dairy Production Conference, Gainesville.

Butler, J. E., Maxwell, C. F., Pierce, C. S., Hylton, M. B., Asofsky, R. and Kiddy, C. A. (1972). Studies on the relative synthesis and distribution of IgG and IgG, in various tissues and body fluids of the cow. J. Immunol. 109:38-46.

Dawson, T. M., and Dawson V. L. (1995). Nitric oxide: actions and pathological roles. Neuroscientist. 1:7–18.

Hirvonen, J., Eklund, K., Teppo, A.M., Huszenicza, G., Kulcsar, M., Saloniemi, H. and Pyo¨ra¨la¨, S. (1999). Acute phase response in dairy cows with experimentally induced E. coli mastitis. Acta Vet Scand.40: 35– 46.

Hobbs AJ, Higgs A, Moncada S. (1999). Inhibition of nitric oxide synthase as a potential therapeutic target. Annu Rev Pharmacol Toxicol. 39:191–200.

Kahl, S., Elsasser, T.H., Blum, J. (1997). Nutritional regulation of plasma tumor necrosis factoralpha and plasma and urinary nitrite/nitrate responses to endotoxin in cattle. Proc. Soc. Exp. Biol. Med. 215: 370–376.

Kinsbergen, M., Bruckmaier, R.M. and Blum, J.W. 1994. Metabolic, endocrine and hematological responses to : 530–547.

Komine ,K., Kuroishi T., Komine Y., Watanabe K., Kobayashi J., Yamabuchi T., Kamata, S. and Kumagi K. (2004). Induction of nitric oxide production mediated by tumor necrosis factor alpha on staphylococcal enterotoxin C- stimulated bovine mammary gland cells. Clin. Diag. Lab. Immunol., 11:203-210

Kushibiki, S., Hodate, K., Shingu, H., Obara, Y., Touno, E., Shinoda, M. and Yokomizo, M. (2003). Metabolic and lactational responses during recombinant bovine tumor necrosis factor-a. J. Dairy Sci. 86: 819–827.

Mackenzie, D. D. S. and Lascelles, A. K. (1968). The transfer of 131 I-labeled immunoglobulins and serum albumin from blood into milk of lactating ewes. Aust. J. Exp. Biol. Med. Sci. 46: 285-294.

Shoker A. S., Yang H., Murabit M. A., Jamil H., Al-Ghoul Ahmed, Okasha K. (1997). Analysis of the in vitro effect of exogenous nitric oxide on human lymphocytes. Molecular and Cellular Biochemistry. 171:75-83.

Stabel, J. R., Goff, J. P. and Kimura, K. (2003). Effects of supplemental energy on metabolic and immune measurements in periparturient dairy cows with Johne’s disease. J. Dairy Sci. 86: 3527–3535.

Yallampalli, C. and Dong, Y. (2000). Estradiol-17â Inhibits Nitric Oxide Synthase (NOS)-II and stimulates NOS-III gene expression in the rat uterus. Biology of Reproduction. 63 : 1 34-41.

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Immunological profiles of lactating and mastitis crossbred cattle

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