SEQUENCE CHARACTERIZATION OF LACTOFERRIN GENE PROMOTER REGION IN BOS INDICUS CATTLE
Citation :- SEQUENCE CHARACTERIZATION OF LACTOFERRIN GENE PROMOTER REGION IN BOS INDICUS CATTLE.Indian Journal Of Animal Research.2014.(48):6-10
Prevalence of several diseases especially mastitis in dairy animals causes reduction in milk production leading to heavy economic loss to the dairy farmers. Lactoferrin (Lf) is a minor whey non-heme iron binding protein produced by the mammary gland may serve a dual role, protecting both the mammary gland and the neonatal intestine from infection. The characterization of lactoferrin gene promoter region has been described in Bos taurus cattle, but there are no reports available in Bos indicus cattle which are widely distributed in Indian sub-continent. About 1kb sequence of lactoferrin gene was generated and the BLAST analysis revealed 99% sequence homology with the exotic cattle sequence. The phylogenetic analysis showed clustering of indicus and taurine cattle together in a single node indicating 5’ regulatory region of lactoferrin gene is highly conserved in these two sub-species. A total of 13 putative transcription factor binding sites were indentified. Although it seems unlikely that all the putative transcription factors identified in the promoter region exert their individual effects, some may actually influence the regulation of bovine lactoferrin gene expression in various tissues especially in mammary gland which need to be further assessed for their functional significance.
Lactoferrin gene promoter mastitis
- Aonymous (2010) Economic survey of India.
- Bahar, B., Halloran, F.O’, Callanan, M. J., McParland, S, Giblin, L and T. Sweeney. (2011). Bovine lactoferrin (LTF) gene promoter haplotypes have different basal transcriptional activities. Anim. Genet., 42 (3): 270-279.
- Brock, J.H. (2002). Lactoferrin- a review. Immun. Today, 23: 222-230.
- Daly, M., Ross, P., Giblin, L and Buckley, F. (2006). Polymorphism within the Lactoferrin gene promoter in various cattle breeds. Anim. Biotechnol., 17: 33-42.
- Hagiwara, S., Kawai, K., Anri, A. and Nagahata H. (2003). Lactoferrin concentrations in milk from normal and subclinical mastitic cows. J. Vet Med. Sci., 65: 319-323.
- Halloran, F.O”., Berry, D.P., Bahar, B., Howard, D.J., Sweeney, T and Giblin, L. (2010). Polymorphisms in the bovine lactoferrin promoter are associated with reproductive performance and science cell count. J.Dairy Sci. 93:1253-159.
- Kawai, K., Hagiwarw, S., Anri, A and Nagahata, H. (1999). Lactoferrin concentration in milk of bovine clinical Mastitis. Vet. Res. Commun., 23: 391-398.
- Kel, A.E., GoBling, E., Reuter, I, Cheremushkin, e, Kel-Margoulis OV and Wingender E (2003) MATCH TM : a tool for searching transcription factor binding sites in DNA sequence. Nucleic Acids Res. 31: 3576-3579.
- Li, Guo-Hua, Yuan Zhang Dong-Xiao Sun and Ning Li. (2004). Study on the Polymorphism of Bovine Lactoferrin Gene and its Relationship with Mastitis. Animal Biotechnology, 15(1): 67-76.
- Sambrook, J.., Fritsh, E.F., and Maniatis, T. (1989). Molecular cloning: A laboratory manual. Cold Spring harbor laboratory press, Cold Spring, N.Y.
- Schug, J and Overton, G C. (1997). Technical report CBIL-TR-1997-1001-v0.0, Computational Biology and Information Laboratory, School of Medicine, University of Pennsylvania.
- Seyfert, Hans-Martin, Andrea Turkoricz., Heidrun Interthal, Dirk Koczan and Gerd Hobom, (1994). Structure of the bovine lactoferrin-encoding gene and its promoter. Gene, 143: 265-269.