Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 48 issue 2 (april 2014) : 198-200,   Doi: 10.5958/j.0976-0555.48.2.041

USE OF VARIOUS METHODS FOR DETECTION OF SERO-GROUP A ROTAVIRUS IN PORCINE INTESTINAL AND FECAL SPECIMENS

M
M. Rout*
G
G. Saikumar
1Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, India
Cite article:- Rout* M., Saikumar G. (2025). USE OF VARIOUS METHODS FOR DETECTION OF SERO-GROUP A ROTAVIRUS IN PORCINE INTESTINAL AND FECAL SPECIMENS. Indian Journal of Animal Research. 48(2): 198-200. doi: 10.5958/j.0976-0555.48.2.041.

ABSTRACT

The study was made to find out the etiological agent involved in piglet diarrhoea during 2007-2009 in and around Bareilly. Group A rotavirus (GARV) was found as the main agent using various methods applied to intestinal and fecal samples. Out of 199 necropsied intestinal samples, none was positive in agarose gel electrophoresis, 5 (2.51%) were positive in double antibody sandwich ELISA (DAS-ELISA), 5 (2.51%) in RT-PCR and 18 (9.04%) in real-time RT PCR. Out of 96 fecal samples, 8 (8.33%) were positive in agarose gel electrophoresis, 11 (11.45%) in DAS-ELISA, 16 (16.66%) in RT-PCR and 23 (23.95%) were found positive in real-time RT PCR. Thus, it was found that real-time RT PCR, RT-PCR, ELISA and agarose gel electrophoresis have a descending order of sensitivity for detection of rotavirus as has already been reported.

REFERENCES

  1. Barman, N.N., Sarma, D.K. and Pensaert, M. (1998). Detection of swine rortavirus and transmissible gastroenteritis virus in piglets with diarrhoea by sandwich ELISA. Indian. J. Anim. Sci., 68: 886-887.
  2. Blacklow, N.R. and Greenberg, H.B. (1991). Viral gastroenteritis. N Engl. J. Med., 325: 252-264.
  3. Buesa, J., Colomina, J., Raga, J.,Villanueva, A. and Prat, J. (1996). Evaluation of reverse transcription and polymerase chain reaction (RT/PCR) for the detection of rotaviruses: applications of the assay. Res. Virol., 147: 353-361.
  4. Hoshino, Y. and Kapikian, A.Z. (1994). Rotavirus antigens. Curr. Top. Microbiol. Immunol., 185: 179-227.
  5. Kalica, A.R., Wyatt, R.G. and Kapikian, A.Z. (1978). Detection of differences among human and animal rotaviruses, using analysis of viral RNA. JAVMA., 173: 531.
  6. Markowska-Daniel, I., Winiarczyk, S., Gradzki, Z. and Pejsak, Z. (1996). Evaluation of different methods (ELISA, IF, EM, PAGE) for the diagnosis of rotavirus infection in piglets. Comp. Immun. Microbiol. Infect. Dis., 19: 219-232.
  7. Mc Neal, M.M., Sestak, K., Choi, A.H., Basu, M., Cole, M.J., Aye, P.P. Bohm, R.P. and Ward, R.L. (2005). Development of a rotavirus shedding model in rhesus macaques, using a homologous wild-type rotavirus of a new P genotype. J. Virol., 79: 944-954.
  8. Moxley, R.A. and Duhamel, G.E. (1999). Comparative pathology of bacterial enteric diseases of swine. Adv. Exp. Med. Biol., 473: 83-101.
  9. Pang, X.L., Lee, B., Boroumand, N., Leblane, B., Preiksaitis, J.K. and Yu Ip, C.C. (2004). Increased detection of rotavirus using a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhoea. J. Med. Virol., 72: 496-501.
  10. Psikal, I., Dvoaak, R., Franz, J. and Snpanek, J. (1991). Rapid identification of bovine rotavirus by electrophoresis in agarose gel. Acta vet. Brno., 60: 253-281.
  11. Schwarz, B.A., Bange, R., Vahlenkamp, T.W., Johne, R. and Muller, H. (2002). Detection and quantitation of group A rotaviruses by competitive and real-time reverse transcription-polymerase chain reaction. J. Virol. Methods., 105: 277–285.
  12. Song, D.S., Kang, B.K., Oh, J.S., Ha, G.W., Yang, J.S., Moon, H.J., Jang, Y.S., and Park, B.K. (2006). Multiplex reverse transcription-PCR for rapid differential detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine group A rotavirus. J. Vet. Diagn. Invest., 18: 278–281.
  13. Zhao, W., Xia, M., Bridges-Malveo, T., Cantu, M., McNeal, M.M., Choi, A.H., Ward, R.L. and Sestak, K. (2005). Evaluation of rotavirus dsRNA load in specimens and body fluids from experimentally infected juvenile macaques by real-time PCR. Virol., 341: 248-256.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 48 issue 2 (april 2014) : 198-200,   Doi: 10.5958/j.0976-0555.48.2.041

    USE OF VARIOUS METHODS FOR DETECTION OF SERO-GROUP A ROTAVIRUS IN PORCINE INTESTINAL AND FECAL SPECIMENS

    M
    M. Rout*
    G
    G. Saikumar
    1Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, India
    Cite article:- Rout* M., Saikumar G. (2025). USE OF VARIOUS METHODS FOR DETECTION OF SERO-GROUP A ROTAVIRUS IN PORCINE INTESTINAL AND FECAL SPECIMENS. Indian Journal of Animal Research. 48(2): 198-200. doi: 10.5958/j.0976-0555.48.2.041.

    ABSTRACT

    The study was made to find out the etiological agent involved in piglet diarrhoea during 2007-2009 in and around Bareilly. Group A rotavirus (GARV) was found as the main agent using various methods applied to intestinal and fecal samples. Out of 199 necropsied intestinal samples, none was positive in agarose gel electrophoresis, 5 (2.51%) were positive in double antibody sandwich ELISA (DAS-ELISA), 5 (2.51%) in RT-PCR and 18 (9.04%) in real-time RT PCR. Out of 96 fecal samples, 8 (8.33%) were positive in agarose gel electrophoresis, 11 (11.45%) in DAS-ELISA, 16 (16.66%) in RT-PCR and 23 (23.95%) were found positive in real-time RT PCR. Thus, it was found that real-time RT PCR, RT-PCR, ELISA and agarose gel electrophoresis have a descending order of sensitivity for detection of rotavirus as has already been reported.

    REFERENCES

    1. Barman, N.N., Sarma, D.K. and Pensaert, M. (1998). Detection of swine rortavirus and transmissible gastroenteritis virus in piglets with diarrhoea by sandwich ELISA. Indian. J. Anim. Sci., 68: 886-887.
    2. Blacklow, N.R. and Greenberg, H.B. (1991). Viral gastroenteritis. N Engl. J. Med., 325: 252-264.
    3. Buesa, J., Colomina, J., Raga, J.,Villanueva, A. and Prat, J. (1996). Evaluation of reverse transcription and polymerase chain reaction (RT/PCR) for the detection of rotaviruses: applications of the assay. Res. Virol., 147: 353-361.
    4. Hoshino, Y. and Kapikian, A.Z. (1994). Rotavirus antigens. Curr. Top. Microbiol. Immunol., 185: 179-227.
    5. Kalica, A.R., Wyatt, R.G. and Kapikian, A.Z. (1978). Detection of differences among human and animal rotaviruses, using analysis of viral RNA. JAVMA., 173: 531.
    6. Markowska-Daniel, I., Winiarczyk, S., Gradzki, Z. and Pejsak, Z. (1996). Evaluation of different methods (ELISA, IF, EM, PAGE) for the diagnosis of rotavirus infection in piglets. Comp. Immun. Microbiol. Infect. Dis., 19: 219-232.
    7. Mc Neal, M.M., Sestak, K., Choi, A.H., Basu, M., Cole, M.J., Aye, P.P. Bohm, R.P. and Ward, R.L. (2005). Development of a rotavirus shedding model in rhesus macaques, using a homologous wild-type rotavirus of a new P genotype. J. Virol., 79: 944-954.
    8. Moxley, R.A. and Duhamel, G.E. (1999). Comparative pathology of bacterial enteric diseases of swine. Adv. Exp. Med. Biol., 473: 83-101.
    9. Pang, X.L., Lee, B., Boroumand, N., Leblane, B., Preiksaitis, J.K. and Yu Ip, C.C. (2004). Increased detection of rotavirus using a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhoea. J. Med. Virol., 72: 496-501.
    10. Psikal, I., Dvoaak, R., Franz, J. and Snpanek, J. (1991). Rapid identification of bovine rotavirus by electrophoresis in agarose gel. Acta vet. Brno., 60: 253-281.
    11. Schwarz, B.A., Bange, R., Vahlenkamp, T.W., Johne, R. and Muller, H. (2002). Detection and quantitation of group A rotaviruses by competitive and real-time reverse transcription-polymerase chain reaction. J. Virol. Methods., 105: 277–285.
    12. Song, D.S., Kang, B.K., Oh, J.S., Ha, G.W., Yang, J.S., Moon, H.J., Jang, Y.S., and Park, B.K. (2006). Multiplex reverse transcription-PCR for rapid differential detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine group A rotavirus. J. Vet. Diagn. Invest., 18: 278–281.
    13. Zhao, W., Xia, M., Bridges-Malveo, T., Cantu, M., McNeal, M.M., Choi, A.H., Ward, R.L. and Sestak, K. (2005). Evaluation of rotavirus dsRNA load in specimens and body fluids from experimentally infected juvenile macaques by real-time PCR. Virol., 341: 248-256.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)