Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 48 issue 2 (april 2014) : 201-203,   Doi: 10.5958/j.0976-0555.48.2.042

EFFECT OF HETEROSPERMY ON THE QUALITY OF SPERMATOZOA DURING PRESERVATION OF BOAR SEMEN AT 180C

K
K. Lalrintluanga*
B
B.C. Deka
K
K.C. Nath
B
B.C. Sarmah
R
R. K. Biswas
1Department of Animal Reproduction, Gynaecology and Obstetrics College of Veterinary Science, Assam Agricultural University, Khanapara-781 022, India
Cite article:- Lalrintluanga* K., Deka B.C., Nath K.C., Sarmah B.C., Biswas K. R. (2025). EFFECT OF HETEROSPERMY ON THE QUALITY OF SPERMATOZOA DURING PRESERVATION OF BOAR SEMEN AT 180C. Indian Journal of Animal Research. 48(2): 201-203. doi: 10.5958/j.0976-0555.48.2.042.

ABSTRACT

A total of 24 ejaculates collected from two Large White Yorkshire (LWY) boars (Boar-A and Boar-B) were diluted at the ratio of 1:8 using Beltsville Thaw Solution extender. From each ejaculate, one third of diluted semen of Boar-A was mixed with one-third of diluted semen of Boar-B and considered as heterospermic semen. The remaining two-third of each ejaculate of Boar-A and Boar-B and the heterospermic semen were preserved for 72 hours at 180C in a BOD incubator. The mean percentage of progressively motile sperm, live sperm, intact acrosome and HOST-reacted sperm evaluated at 0,24,48 and 72 hours of preservation did not differ significantly between individual boar and heterospermic semen, however, progressive sperm motility and live sperm of heterospermic semen were slightly higher than that of one individual boar semen. It was concluded that although heterospermy had marginal effect on certain seminal attributes, the quality of LWY boar semen did not differ significantly between heterospermic semen and individual boar semen.

REFERENCES

  1. Beatty, R.A. (1957). Nigrosin-Eosin staining of rabbit spermatozoa and the fertility of semen. Proc. Rol. Soc. Edin. 67:1.
  2. Ehlers, M.H.; Kuskwaha, N.S. and Erb, R.E. (1958). J. Dairy Sci. 36:1021 In: The Biochemistry of semen and of the male Reproductive Tract. Thaddeus Mann, First Published 1964, Great Britain :Butler and Tanner Ltd, Frome. p. 66
  3. Jeyendran, R.S. ; Van der Ven, H.H. ; Perez-Pelaez, M. ; Crabo, B.G. and Zaneveld, L.J.D. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 70: 219-225.
  4. Ogbuewu, I. P. ; Umesiobi, D.O. ; Okoli, I. C. and Okoro, V.M. (2007). Validation of the polysemen admixture on viability and acrosomal morphology of boars spermatozoa. Online Health and Allied Sci. 6 (1):1-5.
  5. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods. 8th edn. Iowa State University. Ames. Iowa.
  6. Watson, P. F. (1975). Use of a Giemsa stain to detect change in acrosomes of frozen ram spermatozoa. Vet. Rec. 97(1):12.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 48 issue 2 (april 2014) : 201-203,   Doi: 10.5958/j.0976-0555.48.2.042

    EFFECT OF HETEROSPERMY ON THE QUALITY OF SPERMATOZOA DURING PRESERVATION OF BOAR SEMEN AT 180C

    K
    K. Lalrintluanga*
    B
    B.C. Deka
    K
    K.C. Nath
    B
    B.C. Sarmah
    R
    R. K. Biswas
    1Department of Animal Reproduction, Gynaecology and Obstetrics College of Veterinary Science, Assam Agricultural University, Khanapara-781 022, India
    Cite article:- Lalrintluanga* K., Deka B.C., Nath K.C., Sarmah B.C., Biswas K. R. (2025). EFFECT OF HETEROSPERMY ON THE QUALITY OF SPERMATOZOA DURING PRESERVATION OF BOAR SEMEN AT 180C. Indian Journal of Animal Research. 48(2): 201-203. doi: 10.5958/j.0976-0555.48.2.042.

    ABSTRACT

    A total of 24 ejaculates collected from two Large White Yorkshire (LWY) boars (Boar-A and Boar-B) were diluted at the ratio of 1:8 using Beltsville Thaw Solution extender. From each ejaculate, one third of diluted semen of Boar-A was mixed with one-third of diluted semen of Boar-B and considered as heterospermic semen. The remaining two-third of each ejaculate of Boar-A and Boar-B and the heterospermic semen were preserved for 72 hours at 180C in a BOD incubator. The mean percentage of progressively motile sperm, live sperm, intact acrosome and HOST-reacted sperm evaluated at 0,24,48 and 72 hours of preservation did not differ significantly between individual boar and heterospermic semen, however, progressive sperm motility and live sperm of heterospermic semen were slightly higher than that of one individual boar semen. It was concluded that although heterospermy had marginal effect on certain seminal attributes, the quality of LWY boar semen did not differ significantly between heterospermic semen and individual boar semen.

    REFERENCES

    1. Beatty, R.A. (1957). Nigrosin-Eosin staining of rabbit spermatozoa and the fertility of semen. Proc. Rol. Soc. Edin. 67:1.
    2. Ehlers, M.H.; Kuskwaha, N.S. and Erb, R.E. (1958). J. Dairy Sci. 36:1021 In: The Biochemistry of semen and of the male Reproductive Tract. Thaddeus Mann, First Published 1964, Great Britain :Butler and Tanner Ltd, Frome. p. 66
    3. Jeyendran, R.S. ; Van der Ven, H.H. ; Perez-Pelaez, M. ; Crabo, B.G. and Zaneveld, L.J.D. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 70: 219-225.
    4. Ogbuewu, I. P. ; Umesiobi, D.O. ; Okoli, I. C. and Okoro, V.M. (2007). Validation of the polysemen admixture on viability and acrosomal morphology of boars spermatozoa. Online Health and Allied Sci. 6 (1):1-5.
    5. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods. 8th edn. Iowa State University. Ames. Iowa.
    6. Watson, P. F. (1975). Use of a Giemsa stain to detect change in acrosomes of frozen ram spermatozoa. Vet. Rec. 97(1):12.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)