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Current IssueEditorial BoardOnline First ArticlesArchive

volume 48 issue 2 (april 2014) : 129-133,   Doi: 10.5958/j.0976-0555.48.2.028

EFFECT OF TIME AND TEMPERATURE ON THE STORAGE STABILITY OF HEPATOBILIARY ENZYME ACTIVITIES IN CATTLE SERUM

P
P. D. Divya1
K
K.K. Jayavardhanan2
1College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur- 680 651 India
Cite article:- Divya1 D. P., Jayavardhanan2 K.K. (2025). EFFECT OF TIME AND TEMPERATURE ON THE STORAGE STABILITY OF HEPATOBILIARY ENZYME ACTIVITIES IN CATTLE SERUM. Indian Journal of Animal Research. 48(2): 129-133. doi: 10.5958/j.0976-0555.48.2.028.

ABSTRACT

The present research was conducted to choose an ideal storage condition for cattle sera samples meant for the assay of hepatobiliary enzymes viz., Alanine aminotransferases(ALT), Aspartate aminotransferases (AST), Alkaline phosphatase (ALP) and Gamma glutamyltransferases (GGT). The study was performed in adult healthy female crossbred cattle, during the period of April to October 2008.  Stabilities of enzymes in serum stored at room temperature (22 to 27 ºC), 4 ºC and -20 ºC were compared with the enzyme activities of fresh sera samples (day 0) on day 1, 2, 5, 8, 11 and 14.   Alanine aminotransferase was found to be sufficiently stable up to the study period of 14 days at 4 ºC, whereas it was unstable at room temperature and -20 ºC.  Aspartate aminotransferase was stable both at 4 ºC and -20 ºC up to14 days whereas at room temperature stable only up to 2 days. Alkaline phosphatase showed great variation upon storage as compared to other hepatobiliary enzymes and it is suggested that its estimation should be performed in fresh serum samples to get an accurate result. Cattle serum showed a lesser stability for GGT activity at -20 ºC as compared to 4 ºC where the observed stability period was 8 days and 11 days, respectively and at room temperature the enzyme was stable only for one day.  From these results it is therefore advisable to consider stability of each serum hepatobiliary enzymes for different animals separately before preserving sera samples to get more valid and reliable result.

REFERENCES

  1. Benjamin, M.M. (2001). Outline of Veterinary Clinical Pathology. Third edition. Kalyani Publishers, New Delhi, 351pp.
  2. Davy, C.W., Jackson, M.R. and Walker, J.W. (1984). Stabilities of some constituents of Marmoset (Callithrix jacchus) plasma under various conditions of storage. Clin. Chem., 30: 101-104.
  3. Heins, M., Heil, W. and Withold, W. (1995). Storage of serum or whole blood samples, effects of time and temperature on 22 serum analytes. Eur. J. Chem. Clin. Biochem., 33: 231-238.
  4. Kaneko J J, Harvey J W and Bruss M L. (2008). Clinical Biochemistry of Domestic Animals. 6 th edn. Academic Press, California.
  5. Kaplan, L.A. and Pesce, A.J. (1989). Clinical Chemistry, Theory, Analysis and Correlation. Second edition. The C.V. Mosby Company, Baltimore, 1149pp.
  6. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Medhods. 8th edition. Oxford and IBH Publishing Company, Calcutta, 564 pp.
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Indian Journal of Animal Research
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    volume 48 issue 2 (april 2014) : 129-133,   Doi: 10.5958/j.0976-0555.48.2.028

    EFFECT OF TIME AND TEMPERATURE ON THE STORAGE STABILITY OF HEPATOBILIARY ENZYME ACTIVITIES IN CATTLE SERUM

    P
    P. D. Divya1
    K
    K.K. Jayavardhanan2
    1College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur- 680 651 India
    Cite article:- Divya1 D. P., Jayavardhanan2 K.K. (2025). EFFECT OF TIME AND TEMPERATURE ON THE STORAGE STABILITY OF HEPATOBILIARY ENZYME ACTIVITIES IN CATTLE SERUM. Indian Journal of Animal Research. 48(2): 129-133. doi: 10.5958/j.0976-0555.48.2.028.

    ABSTRACT

    The present research was conducted to choose an ideal storage condition for cattle sera samples meant for the assay of hepatobiliary enzymes viz., Alanine aminotransferases(ALT), Aspartate aminotransferases (AST), Alkaline phosphatase (ALP) and Gamma glutamyltransferases (GGT). The study was performed in adult healthy female crossbred cattle, during the period of April to October 2008.  Stabilities of enzymes in serum stored at room temperature (22 to 27 ºC), 4 ºC and -20 ºC were compared with the enzyme activities of fresh sera samples (day 0) on day 1, 2, 5, 8, 11 and 14.   Alanine aminotransferase was found to be sufficiently stable up to the study period of 14 days at 4 ºC, whereas it was unstable at room temperature and -20 ºC.  Aspartate aminotransferase was stable both at 4 ºC and -20 ºC up to14 days whereas at room temperature stable only up to 2 days. Alkaline phosphatase showed great variation upon storage as compared to other hepatobiliary enzymes and it is suggested that its estimation should be performed in fresh serum samples to get an accurate result. Cattle serum showed a lesser stability for GGT activity at -20 ºC as compared to 4 ºC where the observed stability period was 8 days and 11 days, respectively and at room temperature the enzyme was stable only for one day.  From these results it is therefore advisable to consider stability of each serum hepatobiliary enzymes for different animals separately before preserving sera samples to get more valid and reliable result.

    REFERENCES

    1. Benjamin, M.M. (2001). Outline of Veterinary Clinical Pathology. Third edition. Kalyani Publishers, New Delhi, 351pp.
    2. Davy, C.W., Jackson, M.R. and Walker, J.W. (1984). Stabilities of some constituents of Marmoset (Callithrix jacchus) plasma under various conditions of storage. Clin. Chem., 30: 101-104.
    3. Heins, M., Heil, W. and Withold, W. (1995). Storage of serum or whole blood samples, effects of time and temperature on 22 serum analytes. Eur. J. Chem. Clin. Biochem., 33: 231-238.
    4. Kaneko J J, Harvey J W and Bruss M L. (2008). Clinical Biochemistry of Domestic Animals. 6 th edn. Academic Press, California.
    5. Kaplan, L.A. and Pesce, A.J. (1989). Clinical Chemistry, Theory, Analysis and Correlation. Second edition. The C.V. Mosby Company, Baltimore, 1149pp.
    6. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Medhods. 8th edition. Oxford and IBH Publishing Company, Calcutta, 564 pp.
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