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Current IssueEditorial BoardOnline First ArticlesArchive

volume 49 issue 1 (february 2015) : 86-90,   Doi: 10.5958/0976-0555.2015.00018.7

Prevalence and relevance analysis of multidrug-resistant Staphylococcus aureus of meat, poultry and human origin

Y
Yang Fan *
L
Li SM
D
Deng BG
Z
Zhao YX
1Xin Xiang Medical University, School of Basic Medical Sciences, Xinxiangÿ453003, Peoples R China.
Cite article:- * Fan Yang, SM Li, BG Deng, YX Zhao (2025). Prevalence and relevance analysis of multidrug-resistant Staphylococcus aureus of meat, poultry and human origin. Indian Journal of Animal Research. 49(1): 86-90. doi: 10.5958/0976-0555.2015.00018.7.

ABSTRACT

The aim of this study was to investigate the prevalence, antibiotic susceptibility and genotypes of S. aureus of human, meat and poultry origin in Henan Province in China. A total of 327 beef, pork, chicken and human samples collected from local grocery stores and hospital were investigated. The prevalence of S. aureus was found to be 26.7% in beef, 37.5% in pork, 30.1% in chicken and 54.8% in human samples, respectively. Isolates were commonly found resistant to ampicillin (66.9%), tetracycline (61.4%), ciprofloxacin (67.5%) and gentamicin (77.1%), however, none of the isolates was resistant to rifampin and vancomycin, and only one strain of human origin was resistant to vancomycin. The multidrug resistance was common among 66.3% of the isolates. Multilocus sequence typing (MLST) analysis showed that two sequence types namely ST-239 and ST-5 were primarily prevalent among chicken and pork isolates while the most prevalent sequence types among beef and human samples were ST-9 and ST-398, respectively. ST-5 was identified among all isolates, suggesting that the source of S. aureus contamination among animal food and man has some relevance.

REFERENCES

  1. Aarestrup, F.M. (1995). Occurrence of glycopeptide resistance among Enterococcus faecium isolates from conventional and ecological poultry farms. Microb. Drug Resist. 1:255-7.
  2. Bates, J., Jordens, J.Z. and Griths, D.T.(1994). Farm animals as a putative reservoir for vancomycin-resistant enterococcal infection in man. J. Antimicrob. Chemother. 34:507-16.
  3. Borgen, K., Simonsen, G.S., Sundsfjord, A., Wasteson, Y., Olsvik, O. and Kruse, K.(2000). Continuing high prevalence of VanA-type vancomycin-resistant enterococci on Norwegian poultry farms three years after avoparcin was banned. J. Appl. Microbiol. 89:478-85.
  4. Cui S, Li J and Hu C.(2009). Isolation and characterization of methicillin-resistant Staphylococcus aureus from swine and workers in China. J. Antimicrob. Chemother. 64:680–683.
  5. De, B.E., Zwartkruis-Nahuis J.T. and Wit, B.(2009). Prevalence of methicillin-resistant Staphylococcus aureus in meat. Int. J. Food. Microbiol.134:52–6.
  6. Devriese, L.A.(1980). Pathogenic staphylococci in poultry. World. Poultry. Sci. J.36: 227-236.
  7. Enright, M.C. and Spratt, B.G. (1999). Multilocus sequence typing. Trends. Microbiol.7(12):482-7.
  8. Harrison, E.M., Paterson, G.K., Holden, M.T.G., et al(2013). Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC. EMBO. Mol .Med. 5:509–15.
  9. Hasman, H., Moodley, A. and Guardabassi, L.(2010).Spa type distribution in Staphylococcus aureus originating from pigs, cattle and poultry. Vet. Microbiol.141:326–331.
  10. Khanna,T., Friendship, R., Dewey, C. and Weese J.S. (2008). Methicillin resistant Staphylococcus aureus colonization in pigs and pig farmers. Vet. Microbiol.128:298–303.
  11. Kluytmans, J.A.(2010). Methicillin-resistant Staphylococcus aureus in foodproducts: cause for concern or case for complacency? Clin. Microbiol. Infect.16:11–15.
  12. Lee, J.H.(2003). Methicillin (Oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl. Environ. Microbiol.69: 6489–94.
  13. [MLST]. MLST: Staphylococcus aureus. Available at: http://saureus.mlst.net/. Accessed.
  14. Normanno, G., La Salandra, G. and Dambrosio, A.(2007). Occurrence, characterization and antimicrobial resistance of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products. Int. J. Food. Microbiol. 115:290–296.
  15. Pavia, M., Nobile, C.G.A., Salpietro, L. and Angelillo, I.F.(2000). Vancomycin resistance and antibiotic susceptibility of enterococci in raw meat. J. Food. Prot. 63: 912-5.
  16. Price, L.B., Stegger, M., Hasman, H., et al (2012). Staphylococcus aureus CC398: host adaptation and emergence of methicillin resistance in livestock. mBio. 3(1):pii:e00305–11.
  17. Smith,T.C. and Pearson, N.(2011). The emergence of Staphylococcus aureus ST398. Vector. Borne. Zoonotic. Dis. 11(4):327-39.
  18. Tenover, F.C. and McGowan, J.E. Jr. (1996). Reasons for the emergence of antibiotic resistance. Am. J. Med. Sci. 311(1):9-16.
  19. Tenover, F.C. and Hughes, J.M. (1996). The challenges of emerging infectious diseases development and spread of multiply-    resistant bacterial pathogens. J. Am. Med. Assoc. 275(4):300-4.
  20. Van Rijen, M.M., Van Keulen, P.H. and Kluytmans, J.A.(2008). Increase in a Dutch hospital of methicillin-resistant Staphylococcus aureus related to animal farming. Clin. Infect. Dis. 46:261–3.
  21. Van Rijen, M.M, Bosch, T., Verkade, E.J., et al(2014). Livestock-associated MRSA carriage in patients without direct contact with livestock. PloS. ONE. 9(6): e100294.
  22. Wang, X., Meng,J., Zhou,T., et al.(2012). Antimicrobial Susceptibility Testing and Genotypic Characterization of Staphylococcus aureus from Food and Food Animals. Foodborne. Pathog. Dis. 9(2):95-101.
  23. Wang, X., Li, G., Xia, X., Yang, B., Xi, M. and Meng, J.(2014). Antimicrobial susceptibility and molecular typing of methicillin-resistant staphylococcus aureus in retail foods in Shaanxi, China. Foodborne. Pathog. Dis. 11(4):281-6.
  24. Waters, A.E., Contente-Cuomo, T., Buchhagen, J. et al. (2011). Multidrug-Resistant Staphylococcus aureus in US Meat and Poultry. Clin. Infect. Dis. 52(10):1227-30.
  25. Weese, J.S. (2010). Methicillin-Resistant Staphylococcus aureus in Animals. ILAR. J. 51(3):233-44.
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    volume 49 issue 1 (february 2015) : 86-90,   Doi: 10.5958/0976-0555.2015.00018.7

    Prevalence and relevance analysis of multidrug-resistant Staphylococcus aureus of meat, poultry and human origin

    Y
    Yang Fan *
    L
    Li SM
    D
    Deng BG
    Z
    Zhao YX
    1Xin Xiang Medical University, School of Basic Medical Sciences, Xinxiangÿ453003, Peoples R China.
    Cite article:- * Fan Yang, SM Li, BG Deng, YX Zhao (2025). Prevalence and relevance analysis of multidrug-resistant Staphylococcus aureus of meat, poultry and human origin. Indian Journal of Animal Research. 49(1): 86-90. doi: 10.5958/0976-0555.2015.00018.7.

    ABSTRACT

    The aim of this study was to investigate the prevalence, antibiotic susceptibility and genotypes of S. aureus of human, meat and poultry origin in Henan Province in China. A total of 327 beef, pork, chicken and human samples collected from local grocery stores and hospital were investigated. The prevalence of S. aureus was found to be 26.7% in beef, 37.5% in pork, 30.1% in chicken and 54.8% in human samples, respectively. Isolates were commonly found resistant to ampicillin (66.9%), tetracycline (61.4%), ciprofloxacin (67.5%) and gentamicin (77.1%), however, none of the isolates was resistant to rifampin and vancomycin, and only one strain of human origin was resistant to vancomycin. The multidrug resistance was common among 66.3% of the isolates. Multilocus sequence typing (MLST) analysis showed that two sequence types namely ST-239 and ST-5 were primarily prevalent among chicken and pork isolates while the most prevalent sequence types among beef and human samples were ST-9 and ST-398, respectively. ST-5 was identified among all isolates, suggesting that the source of S. aureus contamination among animal food and man has some relevance.

    REFERENCES

    1. Aarestrup, F.M. (1995). Occurrence of glycopeptide resistance among Enterococcus faecium isolates from conventional and ecological poultry farms. Microb. Drug Resist. 1:255-7.
    2. Bates, J., Jordens, J.Z. and Griths, D.T.(1994). Farm animals as a putative reservoir for vancomycin-resistant enterococcal infection in man. J. Antimicrob. Chemother. 34:507-16.
    3. Borgen, K., Simonsen, G.S., Sundsfjord, A., Wasteson, Y., Olsvik, O. and Kruse, K.(2000). Continuing high prevalence of VanA-type vancomycin-resistant enterococci on Norwegian poultry farms three years after avoparcin was banned. J. Appl. Microbiol. 89:478-85.
    4. Cui S, Li J and Hu C.(2009). Isolation and characterization of methicillin-resistant Staphylococcus aureus from swine and workers in China. J. Antimicrob. Chemother. 64:680–683.
    5. De, B.E., Zwartkruis-Nahuis J.T. and Wit, B.(2009). Prevalence of methicillin-resistant Staphylococcus aureus in meat. Int. J. Food. Microbiol.134:52–6.
    6. Devriese, L.A.(1980). Pathogenic staphylococci in poultry. World. Poultry. Sci. J.36: 227-236.
    7. Enright, M.C. and Spratt, B.G. (1999). Multilocus sequence typing. Trends. Microbiol.7(12):482-7.
    8. Harrison, E.M., Paterson, G.K., Holden, M.T.G., et al(2013). Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC. EMBO. Mol .Med. 5:509–15.
    9. Hasman, H., Moodley, A. and Guardabassi, L.(2010).Spa type distribution in Staphylococcus aureus originating from pigs, cattle and poultry. Vet. Microbiol.141:326–331.
    10. Khanna,T., Friendship, R., Dewey, C. and Weese J.S. (2008). Methicillin resistant Staphylococcus aureus colonization in pigs and pig farmers. Vet. Microbiol.128:298–303.
    11. Kluytmans, J.A.(2010). Methicillin-resistant Staphylococcus aureus in foodproducts: cause for concern or case for complacency? Clin. Microbiol. Infect.16:11–15.
    12. Lee, J.H.(2003). Methicillin (Oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl. Environ. Microbiol.69: 6489–94.
    13. [MLST]. MLST: Staphylococcus aureus. Available at: http://saureus.mlst.net/. Accessed.
    14. Normanno, G., La Salandra, G. and Dambrosio, A.(2007). Occurrence, characterization and antimicrobial resistance of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products. Int. J. Food. Microbiol. 115:290–296.
    15. Pavia, M., Nobile, C.G.A., Salpietro, L. and Angelillo, I.F.(2000). Vancomycin resistance and antibiotic susceptibility of enterococci in raw meat. J. Food. Prot. 63: 912-5.
    16. Price, L.B., Stegger, M., Hasman, H., et al (2012). Staphylococcus aureus CC398: host adaptation and emergence of methicillin resistance in livestock. mBio. 3(1):pii:e00305–11.
    17. Smith,T.C. and Pearson, N.(2011). The emergence of Staphylococcus aureus ST398. Vector. Borne. Zoonotic. Dis. 11(4):327-39.
    18. Tenover, F.C. and McGowan, J.E. Jr. (1996). Reasons for the emergence of antibiotic resistance. Am. J. Med. Sci. 311(1):9-16.
    19. Tenover, F.C. and Hughes, J.M. (1996). The challenges of emerging infectious diseases development and spread of multiply-    resistant bacterial pathogens. J. Am. Med. Assoc. 275(4):300-4.
    20. Van Rijen, M.M., Van Keulen, P.H. and Kluytmans, J.A.(2008). Increase in a Dutch hospital of methicillin-resistant Staphylococcus aureus related to animal farming. Clin. Infect. Dis. 46:261–3.
    21. Van Rijen, M.M, Bosch, T., Verkade, E.J., et al(2014). Livestock-associated MRSA carriage in patients without direct contact with livestock. PloS. ONE. 9(6): e100294.
    22. Wang, X., Meng,J., Zhou,T., et al.(2012). Antimicrobial Susceptibility Testing and Genotypic Characterization of Staphylococcus aureus from Food and Food Animals. Foodborne. Pathog. Dis. 9(2):95-101.
    23. Wang, X., Li, G., Xia, X., Yang, B., Xi, M. and Meng, J.(2014). Antimicrobial susceptibility and molecular typing of methicillin-resistant staphylococcus aureus in retail foods in Shaanxi, China. Foodborne. Pathog. Dis. 11(4):281-6.
    24. Waters, A.E., Contente-Cuomo, T., Buchhagen, J. et al. (2011). Multidrug-Resistant Staphylococcus aureus in US Meat and Poultry. Clin. Infect. Dis. 52(10):1227-30.
    25. Weese, J.S. (2010). Methicillin-Resistant Staphylococcus aureus in Animals. ILAR. J. 51(3):233-44.
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