Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 12 (december 2020) : 1490-1496

Comparing the mRNA Expression Profile of Psoas Major and Longissimus Dorsi Muscles in Pig

Yuanyuan Zhao, Guoqing Cao, Pengfei Gao, Guifang Jia, Fei Yang, Jinzhu Meng
1Tongren University, Tongren-554 300, China.
Cite article:- Zhao Yuanyuan, Cao Guoqing, Gao Pengfei, Jia Guifang, Yang Fei, Meng Jinzhu (2020). Comparing the mRNA Expression Profile of Psoas Major and Longissimus Dorsi Muscles in Pig. Indian Journal of Animal Research. 54(12): 1490-1496. doi: 10.18805/ijar.B-1249.

ABSTRACT

To explore the differentially expressed mRNAs between oxidative and glycolytic muscles, Qianbei black pigs were slaughtered and longissimus dorsi muscle (LDM) and psoas major muscle (PMM) were selected and sequenced using Illumina Hiseq TM 4000. Bioinformatics analysis and differentially expressed genes were analyzed by GO and KEGG. qRT-PCR was used to validate the RNA-seq result. As a result, 69 differentially expressed genes were identified, with 46 down regulated genes and 23 up regulated genes in LDM versus PMM, which were categorized into 44 functional groups under three GO classifications. KEGG pathway analysis revealed 20 pathways were enriched. qRT-PCR shows the expression trends of ND6, MYH7, TBX1, FOS and SLC7A5 are consist with the RNA-seq result. We speculated these five genes may involve in differentiation of muscle cells, metabolism of carbohydrate and lipid, deposits of intramuscular fat and transformation of different types of muscle fibers.

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