Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Indian Journal of Animal Research, volume 54 issue 12 (december 2020) : 1470-1475

In vitro Culture of Early Secondary Preantral Follicles to Obtain MII Oocyte Developmental Competence from CD-1 Outbred Mice

Jongwon Kim, Seungki Lee, Jung Kyu Choi
1Department of Biotechnology, College of Life and Applied Sciences, Yeungnam University, Gyeongsan 38541, South Korea. 
Cite article:- Kim Jongwon, Lee Seungki, Choi Kyu Jung (2020). In vitro Culture of Early Secondary Preantral Follicles to Obtain MII Oocyte Developmental Competence from CD-1 Outbred Mice. Indian Journal of Animal Research. 54(12): 1470-1475. doi: 10.18805/IJAR.B-1247.
Background: The ovarian follicle is the fundamental functional tissue unit that consists of mammalian ovary. In humans, it has been known that females are born with a maximum number of follicles or oocytes that are not only non-renewable, but also undergoing degeneration with time with a sharply decreased oocyte quality after the age of 35. 
Methods: Here, we demonstrate that successful isolation of primary, early secondary and late secondary follicles from the ovaries of CD-1 outbred female mice and in vitro culture system to successfully induce the development of MII oocytes. 
Result: The 9 days of in vitro culture of early secondary follicles showed significant higher rates in growth and maturation displaying higher numbers of antral follicles and MII oocytes developed from early secondary follicles compared to those cultured for 11 days. However, there was no visible difference induced by the size of initial follicles in the rates of growth and maturation. MII oocytes derived from in vitro culture of early secondary follicles following in vitro fertilization developed into two-cell embryos. These observations demonstrate that developmentally competent MII oocytes can be obtained by in vitro culture of preantral follicles derived from the ovaries of CD-1 mice and reveal a crucial role for CD-1 mice as a novel model for research on human ovarian follicles. Furthermore, this study proposes an in vitro culture system using preantral follicle as a therapeutic strategy for fertility preservation of humans for assisted reproductive medicine. 
  1. Abir, R., Fisch, B., Nitke, S., Okon, E., Raz, A., Ben Rafael, Z. (2001). Morphological study of fully and partially isolated early human follicles. Fertility and Sterility. 75: 141-146.
  2. Andersen, C.Y., Kristensen, S.G., Greve, T., Schmidt, K.T. (2012). Cryopreservation of ovarian tissue for fertility preservation in young female oncological patients. Future Oncology. 8: 595-608.
  3. Barnett, K.R., Schilling, C., Greenfeld, C.R., Tomic, D., Flaws, J.A. (2006). Ovarian follicle development and transgenic mouse models. Human Reproduction Update. 12: 537-555.
  4. Broekmans, F.J., Soules, M.R., Fauser, B.C. (2009). Ovarian aging: mechanisms and clinical consequences. Endocrine Reviews. 30: 465-493.
  5. Choi, J.K. and He, X. (2013). In vitro maturation of cumulus-oocyte complexes for efficient isolation of oocytes from outbred deer mice. PLoS One. 8(2): e56158.
  6. Choi, J.K., Agarwal, P., He, X. (2013). In vitro culture of early secondary preantral follicles in hanging drop of ovarian cell-conditioned medium to obtain MII oocytes from outbred deer mice. Tissue Engineering Part A. 19: 2626-2637.
  7. Donnez, J. and Dolmans, M.M. (2010). Cryopreservation and transplantation of ovarian tissue. Journal of Clinical Gynecology and Obstetrics. 53: 787-796.
  8. Eppig, J.J. and Schroeder, A.C. (1989). Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation and fertilization in-vitro. Biology of Reproduction. 41: 268-276.
  9. Ernest, H.Y.N., Estella, Y.N.G., Yeung, W.S., Park, C.H. (2001). HMG is as good as recombinant human FSH in terms of oocyte and embryo quality: a prospective randomized trial. Human Reproduction. 16(2): 319-25.
  10. Evelyn, E.T., Zelinski, M.B. (2013). Ovarian Follicle Culture: Advances and Challenges for Human and Non-human Primates. Fertility and Sterility. 99(6): 1523-1533.
  11. Figueiredo, J.R., Cadenas, J., de Lima, L.F., Santos, R.R. (2019). Advances in vitro folliculogenesis in domestic ruminants. Animal Reproduction. 16(1): 52-65.
  12. Goswami, D. and Conway, G.S. (2005). Premature ovarian failure. Human Reproduction Update. 11: 391-410.
  13. Hassold, T. and Hunt, P. (2001). To err (meiotically) is human: the genesis of human aneuploidy. Nature Reviews Genetics. 2: 280-291.
  14. Herta, A.C., Lolicato, F., Smitz, E.J. (2018). In vitro follicle culture in the context of IVF. Reproduction. 156(1): 59-73.
  15. Hreinsson, J.G., Scott, J.E., Rasmussen, C., Swahn, M.L., Hsueh, A.J.W., Hovatta, O. (2002). Growth differentiation factor-9 promotes the growth, development and survival of human ovarian follicles in organ culture. The Journal of Clinical Endocrinology and Metabolism. 87: 316-21.
  16. Jeruss, J.S. and Woodruff, T.K. (2009). Preservation of fertility in patients with cancer. The New England Journal of Medicine. 360: 902-911.
  17. Lee, S.T., Choi, M.H., Gong, S.P., Han, J.Y., Lim, J.M. (2007). Establishment of a basic method for manipulating preantral follicles: effects of retrieval method on in vitro growth of preantral follicles and intrafollicular oocytes. Zygote. 15(2): 109-116.
  18. Liu, J., Van der Elst, J., Van den Broecke, R., Dhont, M. (2001). Live offspring by in vitro fertilization of oocytes from cryopreserved primordial mouse follicles after sequential in vivo transplantation and in vitro maturation. Biology of Reproduction. 64: 171-178.
  19. Malizia, B.A., Hacker, M.R., Penzias, A.S. (2009). Cumulative live-birth rates after in vitro fertilization. The New England Journal of Medicine. 360: 236-243. 
  20. Mott, R. (2007). A haplotype map for the laboratory mouse. Nature Genetics. 39: 1054-56.
  21. Nakagawa, K., Iwasaki, W., Sato, M., Ito, M., Kawachiya, S., Murashima, A., Kitagawa, M., Natori, M., Saito, H. (2005). Successful pregnancy, achieved by ovulation induction using a human menopausal gonadotropin low-dose step-up protocol in an infertile patient with Kallmann’s syndrome. The Journal of Obstetrics and Gynaecology Research. 31(2): 140-43.
  22. Niu, Y. and Liang, S. (2009). Genetic differentiation within the inbred C57BL/6J mouse strain. Journal of Zoology. 278: 42-7.
  23. Oktay, K., Nugent, D., Newton, H., Salha, O., Chatterjee, P., Gosden, R.G. (1997). Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue. Fertility and Sterility. 67: 481-486.
  24. Raj, M.P., Naidu, G.V., Srinivas, M., Raghunath, M. and Rao, K.A. (2018). Effect of preovulatory follicle on fertility in Graded murrah buffaloes (Bubalus bubalis). Journal of Animal Research. 52: 834-838.
  25. Roy, S.K. and Treacy, B.J. (1993). Isolation and long-term culture of human preantral follicles. Fertility and Sterility. 59: 783-790.
  26. Sasan, J.S., Uppal, V. and Bansal, N. (2015). Growing follicle in buffalo ovary: A histomorphometrical study during different seasons of the year. Journal of Animal Research. 49: 317-319.
  27. Smitz, J., Dolmans, M.M., Donnez, J., Fortune, J.E., Hovatta, O., Jewgenow, K. (2010). Current achievements and future research directions in ovarian tissue culture, in vitro follicle development and transplantation: implications for fertility preservation. Human Reproduction Update. 16: 395-414.
  28. Sreejalekshmi, P., Prasad, R.V., Selvaraju, S., Jamuna, K.V. and Sivagnanam, S. (2016). Ultrastructural features of atretic ovarian follicles in buffalo (Bubalus bubalis). Journal of Animal Research. 50: 674-678.
  29. Wade, C.M., Kulbokas, E.J., Kirby, A.W., Zody, M.C., Mullikin, J.C., Lander, E.S. (2002). The mosaic structure of variation in the laboratory mouse genome. Nature. 420: 574-78.
  30. Woodruff, T.K. (2008). Making eggs: is it now or later? Nature Medicine. 14: 1190-1191.
  31. Xu, M., Kreeger, P.K., Shea, L.D., Woodruff, T.K. (2006). Tissue engineered follicles produce live, fertile offspring. Tissue Engineering. 1: 2739-2746.

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