Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Polymorphism of Nramp1 Gene and Its Association with Diarrhea in Pigs

Lang Chen, Shuai Peng, Bao-Qiang Fu, Qian Du, Li Zhang, Li-Xia Liu
1College of Life Science and Engineering, Northwest Minzu University, Lanzhou-730030, China.
  • Submitted12-10-2019|

  • Accepted20-05-2020|

  • First Online 28-07-2020|

  • doi

Cite article:- Chen Lang, Peng Shuai, Fu Bao-Qiang, Du Qian, Zhang Li, Liu Li-Xia (2020). Polymorphism of Nramp1 Gene and Its Association with Diarrhea in Pigs. Indian Journal of Animal Research. 55(7): 786-790. doi: undefined.
Background: Natural resistance associated macrophage protein 1 (Nramp1) is a relatively conservative gene that plays a crucial role in swine immune response and disease resistance. 
Methods: We identified the polymorphisms and gene variations in the exon 2 of Nramp1 using polymerase chain reaction–restriction fragment length polymorphism and investigated the correlation between the polymorphisms and piglet diarrhea in four pig breeds (Bamei, Duroc, Landrace, and Large White pigs). 
Result: The results showed that two alleles (A and B) were identified in all the pig breeds, three genotypes (AA, BB, and AB) were detected in Bamei and Large White breeds, and two genotypes (AA and AB) were detected in Landrace and Duroc breeds. Allele A and genotype AB were dominant in Bamei, Large White, and Landrace breeds, whereas genotype AA was dominant in Duroc pigs. A moderate polymorphism was observed in Landrace and Large White pigs, and polymorphism was abundant in Bamei pigs and low in Duroc pigs. The Chi-square test for Hardy–Weinberg equilibrium disclosed that the exon 2 of Nramp1 in the four breeds of pigs did not deviate from the Hardy–Weinberg balance (P>0.05). The results of association analysis showed a significant correlation between breed and piglet diarrhea (P<0.05), and the diarrhea score of Bamei pigs was much lower than those of the other breeds. The study could supply theoretical references for further functional research on Nramp1 gene and for screening genes related to disease-resistance breeding.
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