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Research Article

volume 54 issue 8 (august 2020) : 936-941,   Doi: 10.18805/ijar.B-1132

Association analysis between microsatellite marker polymorphism and body size trait of Qinghai Bactrian camels

J
Jun. Yan. Bai
W
Wu. Ren. Tao. Di.
J
Jun. Wen. Zhou
W
Wen. Bin
Z
Zhang
H
Hong. Deng. Fan
H
Heng. Cao
X
Xue. Yan. Fu
1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471 003, China.
Cite article:- Bai Yan. Jun., Di. Tao. Ren. Wu., Zhou Wen. Jun., Bin Wen., Zhang, Fan Deng. Hong., Cao Heng., Fu Yan. Xue. (2019). Association analysis between microsatellite marker polymorphism and body size trait of Qinghai Bactrian camels. Indian Journal of Animal Research. 54(8): 936-941. doi: 10.18805/ijar.B-1132.

ABSTRACT

17 microsatellite markers were used in this study to analyze genetic diversity of Qinghai Bactrian camels, conduct association analysis between microsatellite markers and body size or other traits and seek for microsatellite markers related to body size measurement of Qinghai Bactrian camels, thus providing a reference for marker assisted selection of Qinghai Bactrian camels. Results indicated that 17 microsatellite markers totally detected 65 alleles, average number of alleles was 3.0524, average heterozygosity was 0.6205 and average polymorphic information content was 0.5813, showing high polymorphism among the Qinghai Bactrian camel population. Association analysis results indicated that 8 microsatellites—LCA82, CMS18, CMS36, CMS104, CVRL101, YWLL29, YWLL36 and YWLL44—exerted different influences on body weight and body size of Qinghai Bactrian camels, especially LCA82 marker had a significant influence on all body sizes of Qinghai Bactrian camels. Specifically speaking, body height of BB genetic type was significantly higher than that of AA genetic type, chest circumference of BB genetic type was higher than those of AA genetic type and BD genetic type, body length and body weight of BB genetic type were significantly higher than those of AC, BD and AA genetic types, and circumference of cannon bone of BD genetic type was significantly higher than that of AA genetic type. 8 markers—LCA82, CMS18, CMS36, CMS104, CVRL1010, YWLL29, YWLL36 and YWLL44—had significant influences on chest circumference and body weight of Qinghai Bactrian camels. Markers LCA82, CMS36 and YWLL44 had significant influences on their body height, and microsatellite markers LCA82, CMS36, CVRL101 and YWLL44 could exert influences on body length of Qinghai Bactrian camels. 

REFERENCES

  1. Bai, J. Y., Jia, X. P., Yang, Y. B., Zhang, X. H., Pang, Y. Z., Wang, Y. Q. and Qi, Y. X. (2014). Polymorphism analysis of henan fat-    tailed sheep using microsatellite maekers. Journal of Animal & Plant Sciences, 24(3): 965-968.
  2. Bai, J.Y., Pang, Y.Z., Qi, Y.X., Zhang, X.H. and Yun, Y.X. (2017). Analysis of the polymorphism of expressed sequence tag-Simple sequence repeat in quail. Indian Journal of Animal Research, 51 (5): 851-855. DOI:10.18805/ijar.v0i0f.3803.
  3. Bai, J.Y., Pang, Y.Z., Wu, S.J., Yu, M.Q. and Zhang, X.H. (2016a). Analysis of genetic diversity of four quails by microsatellite markers. Indian Journal of Animal Research, 50(1): 1-7. DOI: 10.18805/ijar.8429.
  4. Bai, J.Y., Pang, Y.Z., Zhang, X.H., Yun, Y.X. and Qi, Y.X. (2016b). Microsatellite analysis of genetic diversity in quail populations from China. Brazilian Journal of Poultry Science, 18(3): 519-524. http://dx.doi.org/ 10.1590 /1806-9061-2015-0101.
  5. Bai, J.Y., Pang, Y.Z., Qi, Y.X., Zhang, X.H. and Yun, X.Y. (2016c). Development and application of Est-Ssr Markers in quails, Brazilian Journal of Poultry Science, 18(Special Issue):027-032. http://dx.doi.org/10.1590/1806-9061-2015-0124.
  6. Bai, J.Y., Yang, Y.B., Wang, Y.Q., Zhang, X.H. and Pang, Y.Z. (2015b). Polymorphism analysis of three Chinese indigenous sheep breeds by microsatellite markers. Indian Journal of Animal Research, 49 (5): 585-590. DOI: 10.18805/ijar.5566.
  7. Bai, L. and Feng, D.Z. (2015a). Study on blood physiological and biochemical characteristics of Bactrian camel Chines.Animal Husbandry & Veterinary Medicine, 47(1):103-106.
  8. Banerjee, P., Joshi, J., Upasna, S., Ganai, N. and Vijh, R.K. (2012). Genetic characterisation of two humped camel of India (Camelus bactrianus). Indian Journal of Animal Research, 82(10): 1205-1212.
  9. Barani,A., Rahumathulla, P.S. , Rajendran, R., Kumarasamy, P.,Ganapathi, P. and Radha, P. (2015). Molecular characterization of Pulikulam cattle using microsatellite markers. Indian Journal of Animal Research, 49 (1): 36-39. doi: 10.5958/0976-    0555.2015.00007.2.
  10. Evdotchenko, D., Han, Y., Bartenschlager, H., Preuss, S. and Geldermann, H. (2003). New polymorphic microsatellite loci for different camel species. Molecular Ecology Notes, 3:431-434.https:// doi.org/10.1046/j.1471-8286.2003.00477.x.
  11. Gao, H.W., Wang, J., He, J.X., Chen, L.Y., Jiri, M. and Meng, H. (2009). Analysis on the genetic diversity and evolution in the camelus bactrianus Using SSR Markers. Journal of Shanghai Jiaotong University (Agricultural Science.), 27(2):89-95. 
  12. He, X.H. (2011). China sovereign Bactrian camel population genetic diversity, evolution and heterogeneity of mtDNA. Beijing: a master’s degree thesis of Chinese Academy of Agricultural Sciences, 1-103ÿ
  13. He, X.H., Han, X.L. and Ma, Y.H. (2009). Progress in the study of genetic diversity of bactrian Camel. Journal of Domestic Animal Ecology, 30(4):9-13ÿ
  14. Hedayat-Evrigh, N., Miraei-Ashtiani, S.R., Shahrebabak, M.M.,Evrigh, R.K. and Pourasad, K. (2018). Molecular assessment of genetic diversity in dromedaries and bactrian camel using microsatellite markers. Journal of Agricultural Science and Technology, 20(6): 1137-1148.
  15. Jianlin, H., Mburu, D., Ochieng, J., Kaufmann, B., Rege, J.E. and Hanotte, O. (2000). Application of New World Camelidae microsatellite primers for amplification of polymorphic loci in Old World camelids.Animal Genetics, 31(6):404-406.
  16. Li, X.L. (2012). Study on Meat Characteristics of Alxa Bactrian Camel. Hohhot: a master’s degree thesis of Inner Mongolia Agricultural University.
  17. Ni, W.W., Jiang, A., Zhang, J., Ei, G.X. and Huang, Y, F. (2018). Microsatellite marker-based estimation of the genetic diversity of cattle in Chongqing. Indian Journal of Animal Research, 52(11): 1543-1547. DOI: 10.18805/ijar.B-887.
  18. Sushma, P., Sharique, A.A., Priyanka, B., Jyoti, J., Upasna, S. and Vijh, R.K. (2014). Genetic characterization of Malvi camel using Microsatellite markers. International Journal of Biomedical and Life Sciences, 5(1):286-296.
  19. Tian, Y.Z., Nuerbiya. W., Wang, L.J., Wu, W.W., Xu, X.M., Zhang, Y.H., Azhi. T. and Tian, K.C. (2012). Genetic diversity analysis of 6 Xinjiang local Bactrian camel. Animal Husbandry & Veterinary Medicine, 44(6):38-43. 
  20. Vijh, R.K., Tantia, M.S. and Mishra, B. (2007).Genetic diversity and differentiation of dromedarian camel of India. Animal Biotechnology, 18(2):81-90ÿ
  21. Wuren, T.D., Ba, L., Zhang, W.B., Bao, D., Zhaori, G.T., Bai, J.Y., Su, H., Liu, Q., Zhong, Z.R., Zhai, Y.G., et al (2015). Cluster analysis of body size traits of bactrian camels. Journal of Anhui Agricultural Sciences, 43(2):84-85.
  22. Wuren, T.D., Bai, J.Y., Ba, L., Zhou, J.W. and Zhung, W.B. (2018a). Microsatellite polymorphism and its correlation analysis with body size traits in Alashan Gobi bactrian camel. China Animal Husbandry & Veterinary Medicine, 45(7):1881-1890.
  23. Wuren, T.D., Bai, J.Y., Chu, L., Na, M. and Er, D.M.T. (2017a). Research on growth and development low of Alashan Bactrian camel [J]. Heilongjiang Animal Science and Veterinary Medicine, 11: 248-250. 
  24. Wuren, T.D., Bai, J.Y., Dao, L.M., Bao, D. and Zhang, Q. (2017b). Analysis on the fineness of alashan bactrian camel [J]. Hubei Agricultural Sciences, 56(15):2913-2915. 
  25. Wuren, T.D., Siqin, T.Y. and Bai, J.Y. (2018b). Correlation between microsatellite polymorphism with body size traits of Alashan Desert Bactrian camels. Animal Husbandry & Veterinary Medicine, 50(9):4-9.
  26. Zhang, M.H., Chen, G.L., Zang, C.J. Dong, J., Huang, X.X., Lu, D.L. (2016). Analysis of Physicochemical Indexes of Junggar Bactrian Camel Milk. China Animal Husbandry & Veterinary Medicine, 43(10):2628-2633. 
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Indian Journal of Animal Research
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    Research Article

    volume 54 issue 8 (august 2020) : 936-941,   Doi: 10.18805/ijar.B-1132

    Association analysis between microsatellite marker polymorphism and body size trait of Qinghai Bactrian camels

    J
    Jun. Yan. Bai
    W
    Wu. Ren. Tao. Di.
    J
    Jun. Wen. Zhou
    W
    Wen. Bin
    Z
    Zhang
    H
    Hong. Deng. Fan
    H
    Heng. Cao
    X
    Xue. Yan. Fu
    1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471 003, China.
    Cite article:- Bai Yan. Jun., Di. Tao. Ren. Wu., Zhou Wen. Jun., Bin Wen., Zhang, Fan Deng. Hong., Cao Heng., Fu Yan. Xue. (2019). Association analysis between microsatellite marker polymorphism and body size trait of Qinghai Bactrian camels. Indian Journal of Animal Research. 54(8): 936-941. doi: 10.18805/ijar.B-1132.

    ABSTRACT

    17 microsatellite markers were used in this study to analyze genetic diversity of Qinghai Bactrian camels, conduct association analysis between microsatellite markers and body size or other traits and seek for microsatellite markers related to body size measurement of Qinghai Bactrian camels, thus providing a reference for marker assisted selection of Qinghai Bactrian camels. Results indicated that 17 microsatellite markers totally detected 65 alleles, average number of alleles was 3.0524, average heterozygosity was 0.6205 and average polymorphic information content was 0.5813, showing high polymorphism among the Qinghai Bactrian camel population. Association analysis results indicated that 8 microsatellites—LCA82, CMS18, CMS36, CMS104, CVRL101, YWLL29, YWLL36 and YWLL44—exerted different influences on body weight and body size of Qinghai Bactrian camels, especially LCA82 marker had a significant influence on all body sizes of Qinghai Bactrian camels. Specifically speaking, body height of BB genetic type was significantly higher than that of AA genetic type, chest circumference of BB genetic type was higher than those of AA genetic type and BD genetic type, body length and body weight of BB genetic type were significantly higher than those of AC, BD and AA genetic types, and circumference of cannon bone of BD genetic type was significantly higher than that of AA genetic type. 8 markers—LCA82, CMS18, CMS36, CMS104, CVRL1010, YWLL29, YWLL36 and YWLL44—had significant influences on chest circumference and body weight of Qinghai Bactrian camels. Markers LCA82, CMS36 and YWLL44 had significant influences on their body height, and microsatellite markers LCA82, CMS36, CVRL101 and YWLL44 could exert influences on body length of Qinghai Bactrian camels. 

    REFERENCES

    1. Bai, J. Y., Jia, X. P., Yang, Y. B., Zhang, X. H., Pang, Y. Z., Wang, Y. Q. and Qi, Y. X. (2014). Polymorphism analysis of henan fat-    tailed sheep using microsatellite maekers. Journal of Animal & Plant Sciences, 24(3): 965-968.
    2. Bai, J.Y., Pang, Y.Z., Qi, Y.X., Zhang, X.H. and Yun, Y.X. (2017). Analysis of the polymorphism of expressed sequence tag-Simple sequence repeat in quail. Indian Journal of Animal Research, 51 (5): 851-855. DOI:10.18805/ijar.v0i0f.3803.
    3. Bai, J.Y., Pang, Y.Z., Wu, S.J., Yu, M.Q. and Zhang, X.H. (2016a). Analysis of genetic diversity of four quails by microsatellite markers. Indian Journal of Animal Research, 50(1): 1-7. DOI: 10.18805/ijar.8429.
    4. Bai, J.Y., Pang, Y.Z., Zhang, X.H., Yun, Y.X. and Qi, Y.X. (2016b). Microsatellite analysis of genetic diversity in quail populations from China. Brazilian Journal of Poultry Science, 18(3): 519-524. http://dx.doi.org/ 10.1590 /1806-9061-2015-0101.
    5. Bai, J.Y., Pang, Y.Z., Qi, Y.X., Zhang, X.H. and Yun, X.Y. (2016c). Development and application of Est-Ssr Markers in quails, Brazilian Journal of Poultry Science, 18(Special Issue):027-032. http://dx.doi.org/10.1590/1806-9061-2015-0124.
    6. Bai, J.Y., Yang, Y.B., Wang, Y.Q., Zhang, X.H. and Pang, Y.Z. (2015b). Polymorphism analysis of three Chinese indigenous sheep breeds by microsatellite markers. Indian Journal of Animal Research, 49 (5): 585-590. DOI: 10.18805/ijar.5566.
    7. Bai, L. and Feng, D.Z. (2015a). Study on blood physiological and biochemical characteristics of Bactrian camel Chines.Animal Husbandry & Veterinary Medicine, 47(1):103-106.
    8. Banerjee, P., Joshi, J., Upasna, S., Ganai, N. and Vijh, R.K. (2012). Genetic characterisation of two humped camel of India (Camelus bactrianus). Indian Journal of Animal Research, 82(10): 1205-1212.
    9. Barani,A., Rahumathulla, P.S. , Rajendran, R., Kumarasamy, P.,Ganapathi, P. and Radha, P. (2015). Molecular characterization of Pulikulam cattle using microsatellite markers. Indian Journal of Animal Research, 49 (1): 36-39. doi: 10.5958/0976-    0555.2015.00007.2.
    10. Evdotchenko, D., Han, Y., Bartenschlager, H., Preuss, S. and Geldermann, H. (2003). New polymorphic microsatellite loci for different camel species. Molecular Ecology Notes, 3:431-434.https:// doi.org/10.1046/j.1471-8286.2003.00477.x.
    11. Gao, H.W., Wang, J., He, J.X., Chen, L.Y., Jiri, M. and Meng, H. (2009). Analysis on the genetic diversity and evolution in the camelus bactrianus Using SSR Markers. Journal of Shanghai Jiaotong University (Agricultural Science.), 27(2):89-95. 
    12. He, X.H. (2011). China sovereign Bactrian camel population genetic diversity, evolution and heterogeneity of mtDNA. Beijing: a master’s degree thesis of Chinese Academy of Agricultural Sciences, 1-103ÿ
    13. He, X.H., Han, X.L. and Ma, Y.H. (2009). Progress in the study of genetic diversity of bactrian Camel. Journal of Domestic Animal Ecology, 30(4):9-13ÿ
    14. Hedayat-Evrigh, N., Miraei-Ashtiani, S.R., Shahrebabak, M.M.,Evrigh, R.K. and Pourasad, K. (2018). Molecular assessment of genetic diversity in dromedaries and bactrian camel using microsatellite markers. Journal of Agricultural Science and Technology, 20(6): 1137-1148.
    15. Jianlin, H., Mburu, D., Ochieng, J., Kaufmann, B., Rege, J.E. and Hanotte, O. (2000). Application of New World Camelidae microsatellite primers for amplification of polymorphic loci in Old World camelids.Animal Genetics, 31(6):404-406.
    16. Li, X.L. (2012). Study on Meat Characteristics of Alxa Bactrian Camel. Hohhot: a master’s degree thesis of Inner Mongolia Agricultural University.
    17. Ni, W.W., Jiang, A., Zhang, J., Ei, G.X. and Huang, Y, F. (2018). Microsatellite marker-based estimation of the genetic diversity of cattle in Chongqing. Indian Journal of Animal Research, 52(11): 1543-1547. DOI: 10.18805/ijar.B-887.
    18. Sushma, P., Sharique, A.A., Priyanka, B., Jyoti, J., Upasna, S. and Vijh, R.K. (2014). Genetic characterization of Malvi camel using Microsatellite markers. International Journal of Biomedical and Life Sciences, 5(1):286-296.
    19. Tian, Y.Z., Nuerbiya. W., Wang, L.J., Wu, W.W., Xu, X.M., Zhang, Y.H., Azhi. T. and Tian, K.C. (2012). Genetic diversity analysis of 6 Xinjiang local Bactrian camel. Animal Husbandry & Veterinary Medicine, 44(6):38-43. 
    20. Vijh, R.K., Tantia, M.S. and Mishra, B. (2007).Genetic diversity and differentiation of dromedarian camel of India. Animal Biotechnology, 18(2):81-90ÿ
    21. Wuren, T.D., Ba, L., Zhang, W.B., Bao, D., Zhaori, G.T., Bai, J.Y., Su, H., Liu, Q., Zhong, Z.R., Zhai, Y.G., et al (2015). Cluster analysis of body size traits of bactrian camels. Journal of Anhui Agricultural Sciences, 43(2):84-85.
    22. Wuren, T.D., Bai, J.Y., Ba, L., Zhou, J.W. and Zhung, W.B. (2018a). Microsatellite polymorphism and its correlation analysis with body size traits in Alashan Gobi bactrian camel. China Animal Husbandry & Veterinary Medicine, 45(7):1881-1890.
    23. Wuren, T.D., Bai, J.Y., Chu, L., Na, M. and Er, D.M.T. (2017a). Research on growth and development low of Alashan Bactrian camel [J]. Heilongjiang Animal Science and Veterinary Medicine, 11: 248-250. 
    24. Wuren, T.D., Bai, J.Y., Dao, L.M., Bao, D. and Zhang, Q. (2017b). Analysis on the fineness of alashan bactrian camel [J]. Hubei Agricultural Sciences, 56(15):2913-2915. 
    25. Wuren, T.D., Siqin, T.Y. and Bai, J.Y. (2018b). Correlation between microsatellite polymorphism with body size traits of Alashan Desert Bactrian camels. Animal Husbandry & Veterinary Medicine, 50(9):4-9.
    26. Zhang, M.H., Chen, G.L., Zang, C.J. Dong, J., Huang, X.X., Lu, D.L. (2016). Analysis of Physicochemical Indexes of Junggar Bactrian Camel Milk. China Animal Husbandry & Veterinary Medicine, 43(10):2628-2633. 
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