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Research Article

volume 53 issue 3 (march 2019) : 412-416,   Doi: 10.18805/ijar.B-1057

Effects of the floor type on the gene expression of Hspa1a and cytokines in Holstein dairy cows

A
Abdulkerim DÝLER
1Department of Plant and Animal Production, Erzurum Vocational School, Ataturk University, Erzurum, Turkey.
Cite article:- DÝLER Abdulkerim (2019). Effects of the floor type on the gene expression of Hspa1a and cytokines in Holstein dairy cows. Indian Journal of Animal Research. 53(3): 412-416. doi: 10.18805/ijar.B-1057.

ABSTRACT

This study was carried out to identify the HSPA1A, TNF, IL1B and IL6 mRNA gene expression levels of Holstein dairy cattle sheltered in different floor types. Nineteen Holstein cows were used in this study. The cattle taken into research were divided into two groups as concrete (CON; n= 10) or rubber mat (RUB; n=9). HSPA1A, TNF, IL1B and IL6 mRNA genes are isolated from milk somatic cells and the gene expression is identified by Real-Time PCR. Between the groups, the HSPA1A (P< 0.01) and IL1B (P< 0.05) gene expression levels were found to be statistically significant, while IL6 and TNF gene expressions were not significant. While the IL6 and TNF gene expression differences are insignificant between the groups, numerically higher level of gene expression was observed in the CON group. Overall results of the study suggested that the rubber mat floor type has a positive impact on both the animal welfare and the udder health. 

REFERENCES

  1. Aitken S. L., Karcher E. L., Rezamand P., Gandy J. C., VandeHaar M. J., Capuco A. V., et al (2009). Evaluation of antioxidant and proinflammatory gene expression in bovine mammary tissue during the periparturient period. Journal of Dairy Science, 92(2): 589-598.
  2. Ceyhun S. B., Senturk M., Ekinci D., Erdoðan O., Ciltas A., Kocaman, E. M. (2010). Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 152(2): 215-223.
  3. Earley B., McDonnell B., and O’Riordan E. G. (2015). Effect of floor type on the performance, physiological and behavioural responses of finishing beef steers.Acta Veterinaria Scandinavica, 57(1): 1.
  4. Eicher S. D., Lay D. C., Arthington J. D., and Schutz M. M. (2013). Effects of rubber flooring during the first 2 lactations on production, locomotion, hoof health, immune functions, and stress. Journal of Dairy Science, 96(6): 3639-3651.
  5. Eitam H., Brosh A., Orlov A., Izhaki I., and Shabtay A. (2009). Caloric stress alters fat characteristics and Hsp70 expression in milk somatic cells of lactating beef cows. Cell Stress and Chaperones, 14(2): 173-182.
  6. Fregonesi J. A., Tucker C. B., and Weary D. M. (2007). Overstocking reduces lying time in dairy cows. Journal of Dairy Science, 90(7): 3349-3354.
  7. Haley D. B., De Passille A. M., and Rushen J. (2001). Assessing cow comfort: Effects of two floor types and two tie stall designs on the behaviour of lactating dairy cows. Applied Animal Behaviour Science, 71(2): 105-117.
  8. Koçyigit R. (2014). Farklý zemin tiplerinin esmer sýðýrlarda süt verimi ve süt kompozisyonu ile bazý davranýþ özellikleri üzerine etkileri. PhD Thesis. Ataturk University Institute of Science and Technology Erzurum.
  9. Ma J. L., Zhu Y. H., Zhang, L., Zhuge Z. Y., Liu P. Q., Yan X. D., and Wang J. F. (2011). Serum concentration and mRNA expression in milk somatic cells of toll-like receptor 2, toll-like receptor 4, and cytokines in dairy cows following intramammary inoculation with Escherichia coli. Journal of Dairy Science, 94(12): 5903-5912.
  10. Metcalf J. A., Roberts S. J., and Sutton J. D. (1992). Variations in blood flow to and from the bovine mammary gland measured using transit time ultrasound and dye dilution. Research in Veterinary Science, 53(1): 59-63.
  11. Mitev J., Varlyakov I., Miteva T., Vasilev N., Gergovska J., Uzunova K., and Dimova V. (2012). Preferences of freestall housed dairy cows to different bedding materials. Journal of Faculty of Veterinary Medicine, 38(2): 135-140.
  12. Mogensen L., Krohn C. C., Sørensen J. T., Hindhede J., and Nielsen L. H. (1997). Association between resting behaviour and live weight gain in dairy heifers housed in pens with different space allowance and floor type. Applied Animal Behaviour Science, 55(1): 11-19.
  13. Panivivat R., Kegley E. B., Pennington J. A., Kellogg D. W., and Krumpelman S. L. (2004). Growth performance and health of dairy calves bedded with different types of materials. Journal of Dairy Science, 87(11): 3736-3745.
  14. Parmar, M. S., Madan, A. K., Rastogi, S.K., and Huozha, R. (2015). Expression of heat shock protein 70-1 gene in bovine peripheral blood mononuclear cells. Indian Journal of Animal Research, 49(3): 325-327.
  15. Sailo, L., Gupta, I. D., Verma, A., Das, R., Chaudhari, M. V., and Singh, S. (2016). Polymorphisms in Hsp90ab1 gene and their association with heat tolerance in Sahiwal and Karan Fries cows. Indian Journal of Animal Research, 50(6): 856-861.
  16. Sarikaya H., Prgomet C., Pfaffl M.W., and Bruckmaier R.M. (2004). Differentiation of leukocytes in bovine milk. Milchwissenschaft-    Milk Science International, 59(11-12): 586-589.
  17. Singla M., Sharma A. K., and Parmar O. S. (2007). Effect of bedding material of different depths on milk yield and udder health of cross bred cows during the winter months. Veterinary Practitioner, 8(1): 73-77.
  18. Uthaisangsook S., Day N. K., Bahna S. L., Good R. A., and Haraguchi S. (2002). Innate immunity and its role against infections. Annals of Allergy, Asthma and Immunology, 88(3): 253-265.
  19. Yang, L., Wang, Y., Ma, X., Wang, S., and Zhang, L. (2016). Changes in expression of Th1 and Th2 cytokines in bovine peripheral blood mononuclear cells during early pregnancy. Indian Journal of Animal Research, , 50(4): 466-470. 
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    Research Article

    volume 53 issue 3 (march 2019) : 412-416,   Doi: 10.18805/ijar.B-1057

    Effects of the floor type on the gene expression of Hspa1a and cytokines in Holstein dairy cows

    A
    Abdulkerim DÝLER
    1Department of Plant and Animal Production, Erzurum Vocational School, Ataturk University, Erzurum, Turkey.
    Cite article:- DÝLER Abdulkerim (2019). Effects of the floor type on the gene expression of Hspa1a and cytokines in Holstein dairy cows. Indian Journal of Animal Research. 53(3): 412-416. doi: 10.18805/ijar.B-1057.

    ABSTRACT

    This study was carried out to identify the HSPA1A, TNF, IL1B and IL6 mRNA gene expression levels of Holstein dairy cattle sheltered in different floor types. Nineteen Holstein cows were used in this study. The cattle taken into research were divided into two groups as concrete (CON; n= 10) or rubber mat (RUB; n=9). HSPA1A, TNF, IL1B and IL6 mRNA genes are isolated from milk somatic cells and the gene expression is identified by Real-Time PCR. Between the groups, the HSPA1A (P< 0.01) and IL1B (P< 0.05) gene expression levels were found to be statistically significant, while IL6 and TNF gene expressions were not significant. While the IL6 and TNF gene expression differences are insignificant between the groups, numerically higher level of gene expression was observed in the CON group. Overall results of the study suggested that the rubber mat floor type has a positive impact on both the animal welfare and the udder health. 

    REFERENCES

    1. Aitken S. L., Karcher E. L., Rezamand P., Gandy J. C., VandeHaar M. J., Capuco A. V., et al (2009). Evaluation of antioxidant and proinflammatory gene expression in bovine mammary tissue during the periparturient period. Journal of Dairy Science, 92(2): 589-598.
    2. Ceyhun S. B., Senturk M., Ekinci D., Erdoðan O., Ciltas A., Kocaman, E. M. (2010). Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 152(2): 215-223.
    3. Earley B., McDonnell B., and O’Riordan E. G. (2015). Effect of floor type on the performance, physiological and behavioural responses of finishing beef steers.Acta Veterinaria Scandinavica, 57(1): 1.
    4. Eicher S. D., Lay D. C., Arthington J. D., and Schutz M. M. (2013). Effects of rubber flooring during the first 2 lactations on production, locomotion, hoof health, immune functions, and stress. Journal of Dairy Science, 96(6): 3639-3651.
    5. Eitam H., Brosh A., Orlov A., Izhaki I., and Shabtay A. (2009). Caloric stress alters fat characteristics and Hsp70 expression in milk somatic cells of lactating beef cows. Cell Stress and Chaperones, 14(2): 173-182.
    6. Fregonesi J. A., Tucker C. B., and Weary D. M. (2007). Overstocking reduces lying time in dairy cows. Journal of Dairy Science, 90(7): 3349-3354.
    7. Haley D. B., De Passille A. M., and Rushen J. (2001). Assessing cow comfort: Effects of two floor types and two tie stall designs on the behaviour of lactating dairy cows. Applied Animal Behaviour Science, 71(2): 105-117.
    8. Koçyigit R. (2014). Farklý zemin tiplerinin esmer sýðýrlarda süt verimi ve süt kompozisyonu ile bazý davranýþ özellikleri üzerine etkileri. PhD Thesis. Ataturk University Institute of Science and Technology Erzurum.
    9. Ma J. L., Zhu Y. H., Zhang, L., Zhuge Z. Y., Liu P. Q., Yan X. D., and Wang J. F. (2011). Serum concentration and mRNA expression in milk somatic cells of toll-like receptor 2, toll-like receptor 4, and cytokines in dairy cows following intramammary inoculation with Escherichia coli. Journal of Dairy Science, 94(12): 5903-5912.
    10. Metcalf J. A., Roberts S. J., and Sutton J. D. (1992). Variations in blood flow to and from the bovine mammary gland measured using transit time ultrasound and dye dilution. Research in Veterinary Science, 53(1): 59-63.
    11. Mitev J., Varlyakov I., Miteva T., Vasilev N., Gergovska J., Uzunova K., and Dimova V. (2012). Preferences of freestall housed dairy cows to different bedding materials. Journal of Faculty of Veterinary Medicine, 38(2): 135-140.
    12. Mogensen L., Krohn C. C., Sørensen J. T., Hindhede J., and Nielsen L. H. (1997). Association between resting behaviour and live weight gain in dairy heifers housed in pens with different space allowance and floor type. Applied Animal Behaviour Science, 55(1): 11-19.
    13. Panivivat R., Kegley E. B., Pennington J. A., Kellogg D. W., and Krumpelman S. L. (2004). Growth performance and health of dairy calves bedded with different types of materials. Journal of Dairy Science, 87(11): 3736-3745.
    14. Parmar, M. S., Madan, A. K., Rastogi, S.K., and Huozha, R. (2015). Expression of heat shock protein 70-1 gene in bovine peripheral blood mononuclear cells. Indian Journal of Animal Research, 49(3): 325-327.
    15. Sailo, L., Gupta, I. D., Verma, A., Das, R., Chaudhari, M. V., and Singh, S. (2016). Polymorphisms in Hsp90ab1 gene and their association with heat tolerance in Sahiwal and Karan Fries cows. Indian Journal of Animal Research, 50(6): 856-861.
    16. Sarikaya H., Prgomet C., Pfaffl M.W., and Bruckmaier R.M. (2004). Differentiation of leukocytes in bovine milk. Milchwissenschaft-    Milk Science International, 59(11-12): 586-589.
    17. Singla M., Sharma A. K., and Parmar O. S. (2007). Effect of bedding material of different depths on milk yield and udder health of cross bred cows during the winter months. Veterinary Practitioner, 8(1): 73-77.
    18. Uthaisangsook S., Day N. K., Bahna S. L., Good R. A., and Haraguchi S. (2002). Innate immunity and its role against infections. Annals of Allergy, Asthma and Immunology, 88(3): 253-265.
    19. Yang, L., Wang, Y., Ma, X., Wang, S., and Zhang, L. (2016). Changes in expression of Th1 and Th2 cytokines in bovine peripheral blood mononuclear cells during early pregnancy. Indian Journal of Animal Research, , 50(4): 466-470. 
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