Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 6 (june 2020) : 679-684

Molecular cloning, characterization and tissue specificity of the expression in the TAC1 genes from Henan Huai goat (Capra hircus)

Zhilong Tian, Yuqin Wang, Huibin Shi, Zhibo Wu, Xiaohui Zhang, Jianping Wang, Yuanxiao Li, Fang Yang, Yumei Liu, Mingxing Chu
1College of Animal Science and Technology,<br />Henan University of Science and Technology, Luoyang-471 003, China.
Cite article:- Tian Zhilong, Wang Yuqin, Shi Huibin, Wu Zhibo, Zhang Xiaohui, Wang Jianping, Li Yuanxiao, Yang Fang, Liu Yumei, Chu Mingxing (2019). Molecular cloning, characterization and tissue specificity of the expression in the TAC1 genes from Henan Huai goat (Capra hircus). Indian Journal of Animal Research. 54(6): 679-684. doi: 10.18805/ijar.B-1025.

ABSTRACT

To further to understand the structure and function of the TAC1 gene, we cloned the full-length cDNAs of the TAC1 genes from goat by rapid amplification of cDNA ends-PCR and the qRT-PCR was used to analyze the TAC1 mRNA expression patterns of goat various tissues. The full-length cDNA of goat TAC1 was 1176 bp, with a 339 bp open reading frame encoding 112 amino acids. The amino acid sequence analysis revealed that goat TAC1 gene encoded a water-drain protein and its relative molecular weight and isoelectric point was 13,012.86 Da and 6.29 respectively. Alignment and phylogenetic analyses revealed that their amino acid sequences were highly similar to those of other vertebrates. TAC1 expression of the goat of the brain, cerebellum, medulla oblongata, heart, liver, spleen, lung, kidney, uterus, ovaries. These results serve as a foundation for further study on the Capra hircus TAC1 gene.

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