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volume 46 issue 4 (december 2012) : 325 - 333

EVALUATION OF IMMUNOCONTRACEPTIVE PROPERTY OF SPERMATOZOA IN HOUSE RAT (Rattus rattus)

D
D.K. Kocher*
S
S. Garg
M
M. Chandra
J
J. Kapur
1Department of Zoology, Punjab Agricultural University, Ludhiana-141 004, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Kocher* D.K., Garg S., Chandra M., Kapur J. (2025). EVALUATION OF IMMUNOCONTRACEPTIVE PROPERTY OF SPERMATOZOA IN HOUSE RAT (Rattus rattus). Indian Journal of Animal Research. 46(4): 325 - 333. doi: .

ABSTRACT

Immunization of house rats with three antigenic preparations having 1, 5 and 10 million spermatozoa, resulted in maximum production of antisperm-antibodies (ASA) after 3rd intradermal injection. The highest value of %ASA was found to be 26.11±0.52 after immunization with 5 million spermatozoa at 28 days post immunization (DPI) as compared to other two sperm antigens. While ELISA identified maximally the production of ASA at 21 DPI in the serum of house rats immunized with 5 million sperms. Study of various sperm parameters revealed a significant reduction in sperm concentration (million/ml) from 161.84±18.79 (control) to 11.69±4.09, per cent sperm motility from 88.33±7.26 (control) to 22.33±11.57 and increase in per cent sperm abnormalities from 1.55±0.45 (control) to 68.15±4.19 after immunization with antigen having 5 million spermatozoa., indicated that this antigenic preparation can be used as immunocontraceptive agent for the management of house rat in future.

REFERENCES

  1. Alexander, N.J. (1984) Antibodies to human spermatoza impede penetration of cervical mucus or hamster eggs fertile steril 41 : 433-439.
  2. Buckle, A. P., Prescott, C.V and Ward, K. J. (1994). Resistance to the first and second generation anticoagulant rodenticides- A new perspective. Proc. 16th Vetebr. Pest Conf. pp 138-142.
  3. Busso, D., Cohen, D. J., Maldera, J. A., Dematteis, A., and Cuasnicu, P. S. (2007). A novel function for CRISPI in rodent fertilization: Involvement in sperm-zona pellucida interaction. Biol. Reprod. 107: 061788 (on line).
  4. Chen, Z., He, W., Liang, Z., Yan, P., He, H., Tang, Y., Zhang, J., Shen, Z., Ni. B., Wu. Y., and Li, J. (2009). Protein Prime-Peptide Boost as a new strategy induced an Epin dominant B-cell epitope specific immune response and suppressed fertility. Vaccine, 27(5): 733-740.
  5. Cooper, W., and Larsen, E. (2006). Immunocontraception of mammalian wildlife: Ecological and Immunogenetic issues. Reproduction, 132: 821-828.
  6. Crowther, J. R.(1995). Methods in molecular biology: ELISA, Theory and Practice, vol. 42 Humana Press (New Jersey, Product code 236, 415-0).
  7. Cuasnicu, P. S., Echeverria, F. G., Piazza, A. D., Cameo, M. S., and Blaquier, J. A. (1984). Antibodies against epididymal glycoproteins block fertilizing ability in rat. Reproduction, 72 : 467-471.
  8. Ellerman, D. A., Brantua, V. S., Martinez, S. P., Cohen, D. J., Conesa, D., and Cuasnicu, P. S. (1998). Potential contraceptive use of epididymal proteins: Immunization of male rats with epididymal protein DE inhibits sperm fusial ability. Biol. Reprod. 59: 1029-1036.
  9. Hinton, B. T., and Howards, S. S. (1981). Permeability characteristics of the epithelium in the rat caput epididymis. J. Reprod. Ferti. 63: 95-99.
  10. Irvine, D. S., and Aitken, R. J. (1994). Seminal fluid analysis and sperm function testing. Endocrinology and Metabolism Clinics of North America. 23(4), 725-748.
  11. Kaur, R., and Parshad, V. R. (1994). Effects of dietary selenium on differentiation, morphology and functions of spermatozoa of the house rat, (Rattus rattus) L. Mut. Res. 309: 29-35.
  12. Khan, S. A., Suryawanshi, A. R., Ranpura, S. A., Jadhav, S. V., and Khole, V. V. (2009). Identification of Novel Immunodominant Epididymal sperm proteins using combinatorial approach. Reproduction. 138(1) : 81-93.
  13. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, A. J. (1951). Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265-275.
  14. Miller, L. A. (1995). Immunocontraception and possible application in wildlife damage management. Proc.Great Plains Wildlife Damage control workshop pp 27-30. University of Nebraska, Lincoln.
  15. Naz, R. K., and Zhu, X. (1998). Recombinant Fertilisation Antigen-1 causes a contraceptive effect in actively immunized nice. Biol.Reprod. 59 : 1095-1100.
  16. Naz, R. K., Gupta, J. C., Vyas, H. K., and Talwar, G. P. (2005). Mini Review: Development in Reproductive Medicine. Human Reprod. 20: 3271-3283.
  17. Parshad, V. R. (1999). Rodent control in India. Int. Pest Mgmt. Rev. 4: 97-126.
  18. Risvanli, A., Timurkan, H., Aydin, M., and Balut, H. (2003). Use of cauda epididymis extract for immunocontraception in male rats. Indian Vet. J. 80: 1142-1144.
  19. Ryder, T., Mobberley, M., Hughes, L., and Hendry, W. A. (1990). Survey of the ultrastructural defects associated with absent or impaired human sperm motility. Fertil. Steril. 53 : 556-560.
  20. Salisbury, G. W., Denmark, N. L., and Lodge, J. R. (1978). Physiology of Reproduction and Artificial Insemination of Cattle. 2nd ed. W H Freeman and company, San Francisco.
  21. Suri, A. (2005). Contraceptive vaccines targeting sperm. Expert opinion on Biological Therapy 5 (3): 381-392.
  22. Zouari, R.,and Almeida, M. D. (1993). Effect of sperm-associated antibodies on human sperm ability to bind to zona pellucida and to penetrate zona free hamster oocytes. J. Reprod. Immunol. 24: 175-186.
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    volume 46 issue 4 (december 2012) : 325 - 333

    EVALUATION OF IMMUNOCONTRACEPTIVE PROPERTY OF SPERMATOZOA IN HOUSE RAT (Rattus rattus)

    D
    D.K. Kocher*
    S
    S. Garg
    M
    M. Chandra
    J
    J. Kapur
    1Department of Zoology, Punjab Agricultural University, Ludhiana-141 004, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Kocher* D.K., Garg S., Chandra M., Kapur J. (2025). EVALUATION OF IMMUNOCONTRACEPTIVE PROPERTY OF SPERMATOZOA IN HOUSE RAT (Rattus rattus). Indian Journal of Animal Research. 46(4): 325 - 333. doi: .

    ABSTRACT

    Immunization of house rats with three antigenic preparations having 1, 5 and 10 million spermatozoa, resulted in maximum production of antisperm-antibodies (ASA) after 3rd intradermal injection. The highest value of %ASA was found to be 26.11±0.52 after immunization with 5 million spermatozoa at 28 days post immunization (DPI) as compared to other two sperm antigens. While ELISA identified maximally the production of ASA at 21 DPI in the serum of house rats immunized with 5 million sperms. Study of various sperm parameters revealed a significant reduction in sperm concentration (million/ml) from 161.84±18.79 (control) to 11.69±4.09, per cent sperm motility from 88.33±7.26 (control) to 22.33±11.57 and increase in per cent sperm abnormalities from 1.55±0.45 (control) to 68.15±4.19 after immunization with antigen having 5 million spermatozoa., indicated that this antigenic preparation can be used as immunocontraceptive agent for the management of house rat in future.

    REFERENCES

    1. Alexander, N.J. (1984) Antibodies to human spermatoza impede penetration of cervical mucus or hamster eggs fertile steril 41 : 433-439.
    2. Buckle, A. P., Prescott, C.V and Ward, K. J. (1994). Resistance to the first and second generation anticoagulant rodenticides- A new perspective. Proc. 16th Vetebr. Pest Conf. pp 138-142.
    3. Busso, D., Cohen, D. J., Maldera, J. A., Dematteis, A., and Cuasnicu, P. S. (2007). A novel function for CRISPI in rodent fertilization: Involvement in sperm-zona pellucida interaction. Biol. Reprod. 107: 061788 (on line).
    4. Chen, Z., He, W., Liang, Z., Yan, P., He, H., Tang, Y., Zhang, J., Shen, Z., Ni. B., Wu. Y., and Li, J. (2009). Protein Prime-Peptide Boost as a new strategy induced an Epin dominant B-cell epitope specific immune response and suppressed fertility. Vaccine, 27(5): 733-740.
    5. Cooper, W., and Larsen, E. (2006). Immunocontraception of mammalian wildlife: Ecological and Immunogenetic issues. Reproduction, 132: 821-828.
    6. Crowther, J. R.(1995). Methods in molecular biology: ELISA, Theory and Practice, vol. 42 Humana Press (New Jersey, Product code 236, 415-0).
    7. Cuasnicu, P. S., Echeverria, F. G., Piazza, A. D., Cameo, M. S., and Blaquier, J. A. (1984). Antibodies against epididymal glycoproteins block fertilizing ability in rat. Reproduction, 72 : 467-471.
    8. Ellerman, D. A., Brantua, V. S., Martinez, S. P., Cohen, D. J., Conesa, D., and Cuasnicu, P. S. (1998). Potential contraceptive use of epididymal proteins: Immunization of male rats with epididymal protein DE inhibits sperm fusial ability. Biol. Reprod. 59: 1029-1036.
    9. Hinton, B. T., and Howards, S. S. (1981). Permeability characteristics of the epithelium in the rat caput epididymis. J. Reprod. Ferti. 63: 95-99.
    10. Irvine, D. S., and Aitken, R. J. (1994). Seminal fluid analysis and sperm function testing. Endocrinology and Metabolism Clinics of North America. 23(4), 725-748.
    11. Kaur, R., and Parshad, V. R. (1994). Effects of dietary selenium on differentiation, morphology and functions of spermatozoa of the house rat, (Rattus rattus) L. Mut. Res. 309: 29-35.
    12. Khan, S. A., Suryawanshi, A. R., Ranpura, S. A., Jadhav, S. V., and Khole, V. V. (2009). Identification of Novel Immunodominant Epididymal sperm proteins using combinatorial approach. Reproduction. 138(1) : 81-93.
    13. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, A. J. (1951). Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265-275.
    14. Miller, L. A. (1995). Immunocontraception and possible application in wildlife damage management. Proc.Great Plains Wildlife Damage control workshop pp 27-30. University of Nebraska, Lincoln.
    15. Naz, R. K., and Zhu, X. (1998). Recombinant Fertilisation Antigen-1 causes a contraceptive effect in actively immunized nice. Biol.Reprod. 59 : 1095-1100.
    16. Naz, R. K., Gupta, J. C., Vyas, H. K., and Talwar, G. P. (2005). Mini Review: Development in Reproductive Medicine. Human Reprod. 20: 3271-3283.
    17. Parshad, V. R. (1999). Rodent control in India. Int. Pest Mgmt. Rev. 4: 97-126.
    18. Risvanli, A., Timurkan, H., Aydin, M., and Balut, H. (2003). Use of cauda epididymis extract for immunocontraception in male rats. Indian Vet. J. 80: 1142-1144.
    19. Ryder, T., Mobberley, M., Hughes, L., and Hendry, W. A. (1990). Survey of the ultrastructural defects associated with absent or impaired human sperm motility. Fertil. Steril. 53 : 556-560.
    20. Salisbury, G. W., Denmark, N. L., and Lodge, J. R. (1978). Physiology of Reproduction and Artificial Insemination of Cattle. 2nd ed. W H Freeman and company, San Francisco.
    21. Suri, A. (2005). Contraceptive vaccines targeting sperm. Expert opinion on Biological Therapy 5 (3): 381-392.
    22. Zouari, R.,and Almeida, M. D. (1993). Effect of sperm-associated antibodies on human sperm ability to bind to zona pellucida and to penetrate zona free hamster oocytes. J. Reprod. Immunol. 24: 175-186.
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